ENSG00000027697

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367739 ENSG00000027697 HEK293_OSMI2_2hA HEK293_TMG_2hB IFNGR1 protein_coding protein_coding 30.52399 8.240886 42.76589 1.109515 0.7525755 2.374177 20.281944 5.543357 33.121855 0.4991171 1.6855972 2.5767868 0.6757208 0.7135667 0.77393333 0.06036667 9.942704e-01 1.023731e-17 FALSE TRUE
ENST00000696693 ENSG00000027697 HEK293_OSMI2_2hA HEK293_TMG_2hB IFNGR1 protein_coding protein_coding 30.52399 8.240886 42.76589 1.109515 0.7525755 2.374177 4.929276 2.382022 1.848353 1.4245337 1.8483534 -0.3642056 0.2064875 0.2525667 0.04356667 -0.20900000 3.430433e-01 1.023731e-17 FALSE TRUE
MSTRG.29025.18 ENSG00000027697 HEK293_OSMI2_2hA HEK293_TMG_2hB IFNGR1 protein_coding   30.52399 8.240886 42.76589 1.109515 0.7525755 2.374177 4.181967 0.000000 6.149476 0.0000000 0.6165431 9.2666638 0.0774250 0.0000000 0.14413333 0.14413333 1.023731e-17 1.023731e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000027697 E001 0.2214452 0.0416253466 9.344726e-02   6 137197483 137197484 2 - 0.000 0.225 11.292
ENSG00000027697 E002 0.8814403 0.0136968632 8.811635e-05 4.661523e-04 6 137197485 137197496 12 - 0.000 0.643 15.864
ENSG00000027697 E003 333.7071952 0.0001750950 2.650841e-21 1.963996e-19 6 137197497 137198133 637 - 2.394 2.590 0.656
ENSG00000027697 E004 424.2295247 0.0008437434 8.511848e-03 2.506675e-02 6 137198134 137198639 506 - 2.535 2.592 0.192
ENSG00000027697 E005 150.6213007 0.0003859515 8.511678e-02 1.675999e-01 6 137200881 137201008 128 - 2.085 2.144 0.198
ENSG00000027697 E006 1.2082949 0.2775735476 8.023304e-01 8.740755e-01 6 137202863 137202903 41 - 0.320 0.225 -0.678
ENSG00000027697 E007 103.5706841 0.0002629169 7.937614e-01 8.681741e-01 6 137203499 137203532 34 - 1.936 1.947 0.038
ENSG00000027697 E008 187.6127685 0.0002240976 6.424384e-02 1.337732e-01 6 137203533 137203642 110 - 2.207 2.148 -0.196
ENSG00000027697 E009 144.8308423 0.0002352508 1.739721e-02 4.581752e-02 6 137203643 137203685 43 - 2.102 2.015 -0.292
ENSG00000027697 E010 1.6522440 0.0090454152 4.950143e-01 6.318954e-01 6 137203686 137203730 45 - 0.352 0.482 0.704
ENSG00000027697 E011 259.0086817 0.0003145897 4.318233e-05 2.470410e-04 6 137204332 137204504 173 - 2.356 2.242 -0.382
ENSG00000027697 E012 161.9865115 0.0002949601 1.017128e-03 4.018098e-03 6 137206136 137206206 71 - 2.152 2.034 -0.393
ENSG00000027697 E013 145.3442571 0.0015880040 7.476047e-03 2.245161e-02 6 137206207 137206308 102 - 2.104 1.992 -0.375
ENSG00000027697 E014 0.7342825 0.0159502189 3.426364e-01 4.866436e-01 6 137206642 137206962 321 - 0.250 0.000 -13.766
ENSG00000027697 E015 132.4825977 0.0015814107 2.231934e-02 5.633871e-02 6 137206963 137207077 115 - 2.063 1.966 -0.326
ENSG00000027697 E016 0.8909948 0.0136968632 9.456401e-01 9.698916e-01 6 137209850 137209972 123 - 0.251 0.225 -0.203
ENSG00000027697 E017 0.1472490 0.0442577546 1.000000e+00   6 137209973 137209984 12 - 0.063 0.000 -11.210
ENSG00000027697 E018 0.0000000       6 137213751 137213832 82 -      
ENSG00000027697 E019 2.0627580 0.0364869999 2.352085e-01 3.686049e-01 6 137215267 137215377 111 - 0.481 0.225 -1.583
ENSG00000027697 E020 0.1472490 0.0442577546 1.000000e+00   6 137218028 137218060 33 - 0.063 0.000 -11.210
ENSG00000027697 E021 0.5943067 0.0227219742 7.533624e-01 8.393393e-01 6 137218451 137218671 221 - 0.167 0.225 0.533
ENSG00000027697 E022 5.9284296 0.0502906903 7.390648e-01 8.287919e-01 6 137218672 137218983 312 - 0.777 0.709 -0.277
ENSG00000027697 E023 3.1721766 0.0384107624 7.020820e-01 8.009739e-01 6 137218984 137219093 110 - 0.563 0.485 -0.370
ENSG00000027697 E024 1.8446316 0.0926166736 5.208876e-01 6.548322e-01 6 137219094 137219242 149 - 0.352 0.488 0.733
ENSG00000027697 E025 91.7798322 0.0071283218 2.858385e-03 9.848547e-03 6 137219243 137219449 207 - 1.922 1.725 -0.662