ENSG00000025796

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369002 ENSG00000025796 HEK293_OSMI2_2hA HEK293_TMG_2hB SEC63 protein_coding protein_coding 45.80001 23.11419 67.98847 0.4248431 2.682515 1.5561 3.268090 1.9214326 5.006136 0.09926774 0.0641667 1.376905 0.07702917 0.08333333 0.07376667 -0.009566667 6.603431e-01 6.439037e-10 FALSE TRUE
MSTRG.28736.2 ENSG00000025796 HEK293_OSMI2_2hA HEK293_TMG_2hB SEC63 protein_coding   45.80001 23.11419 67.98847 0.4248431 2.682515 1.5561 32.164543 19.4338687 44.104133 0.23212063 2.7355199 1.181926 0.73144167 0.84103333 0.64770000 -0.193333333 6.439037e-10 6.439037e-10 FALSE TRUE
MSTRG.28736.4 ENSG00000025796 HEK293_OSMI2_2hA HEK293_TMG_2hB SEC63 protein_coding   45.80001 23.11419 67.98847 0.4248431 2.682515 1.5561 7.689466 0.6691274 15.076375 0.39327497 1.1006556 4.473420 0.12087500 0.02893333 0.22370000 0.194766667 4.191836e-04 6.439037e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000025796 E001 222.9789343 0.0002317477 6.883247e-02 1.414359e-01 6 107867756 107870764 3009 - 2.290 2.369 0.262
ENSG00000025796 E002 1273.0986437 0.0037997859 1.152473e-13 3.246098e-12 6 107870765 107871847 1083 - 2.985 3.217 0.769
ENSG00000025796 E003 281.6985042 0.0001943084 1.747629e-04 8.558847e-04 6 107872808 107872884 77 - 2.376 2.493 0.389
ENSG00000025796 E004 203.0092312 0.0002011303 8.571421e-04 3.462915e-03 6 107872885 107872912 28 - 2.235 2.355 0.401
ENSG00000025796 E005 366.5065850 0.0002592661 1.041864e-05 6.898435e-05 6 107876564 107876662 99 - 2.489 2.611 0.405
ENSG00000025796 E006 0.7437457 0.0153787590 6.869366e-01 7.893663e-01 6 107876663 107877400 738 - 0.251 0.188 -0.538
ENSG00000025796 E007 1.2867575 0.0106573143 8.490194e-01 9.062926e-01 6 107880960 107881148 189 - 0.338 0.318 -0.120
ENSG00000025796 E008 416.4729429 0.0001530490 4.065928e-03 1.332684e-02 6 107881149 107881250 102 - 2.557 2.643 0.285
ENSG00000025796 E009 444.0552399 0.0001741157 2.475759e-02 6.137868e-02 6 107882988 107883146 159 - 2.589 2.662 0.242
ENSG00000025796 E010 2.3509358 0.0070479838 3.280663e-01 4.715103e-01 6 107883147 107883467 321 - 0.441 0.627 0.878
ENSG00000025796 E011 263.6302508 0.0010447773 6.756875e-01 7.808896e-01 6 107893482 107893579 98 - 2.373 2.415 0.142
ENSG00000025796 E012 236.8054932 0.0001758677 5.184764e-02 1.124498e-01 6 107893580 107893655 76 - 2.346 2.328 -0.061
ENSG00000025796 E013 2.1152093 0.0075214308 5.742299e-01 7.000776e-01 6 107893656 107893837 182 - 0.499 0.418 -0.412
ENSG00000025796 E014 220.3623441 0.0001911968 1.668754e-02 4.425073e-02 6 107893838 107893897 60 - 2.320 2.288 -0.108
ENSG00000025796 E015 256.1105515 0.0002280026 3.301744e-03 1.115401e-02 6 107897649 107897731 83 - 2.387 2.345 -0.140
ENSG00000025796 E016 280.2603045 0.0021689572 7.860429e-02 1.572609e-01 6 107901370 107901517 148 - 2.420 2.394 -0.085
ENSG00000025796 E017 245.8672057 0.0021221501 4.772295e-01 6.158323e-01 6 107902844 107902994 151 - 2.352 2.360 0.025
ENSG00000025796 E018 105.1289681 0.0017678315 4.843765e-01 6.223962e-01 6 107902995 107902998 4 - 1.986 1.987 0.002
ENSG00000025796 E019 207.7737684 0.0002222927 3.030034e-01 4.449254e-01 6 107904629 107904721 93 - 2.281 2.284 0.010
ENSG00000025796 E020 120.6252411 0.0002664127 6.365962e-02 1.327944e-01 6 107906448 107906464 17 - 2.060 2.025 -0.119
ENSG00000025796 E021 236.5491675 0.0003275719 8.598259e-02 1.689623e-01 6 107906465 107906580 116 - 2.343 2.329 -0.047
ENSG00000025796 E022 218.3086269 0.0002947758 1.136449e-02 3.203643e-02 6 107906683 107906777 95 - 2.315 2.277 -0.127
ENSG00000025796 E023 215.1323155 0.0002724870 1.659000e-05 1.048096e-04 6 107908927 107909007 81 - 2.323 2.234 -0.297
ENSG00000025796 E024 152.1346884 0.0029313079 1.007039e-05 6.686073e-05 6 107909008 107909035 28 - 2.189 2.031 -0.527
ENSG00000025796 E025 0.4355181 0.6099659249 6.439819e-01 7.564018e-01 6 107909036 107909159 124 - 0.200 0.000 -11.090
ENSG00000025796 E026 0.7385489 0.0426881939 1.520960e-01 2.645415e-01 6 107911168 107911345 178 - 0.297 0.000 -11.831
ENSG00000025796 E027 137.8656621 0.0002577239 3.296901e-07 3.024347e-06 6 107911346 107911396 51 - 2.145 1.997 -0.496
ENSG00000025796 E028 151.4808604 0.0002460544 3.453088e-06 2.551194e-05 6 107912716 107912774 59 - 2.180 2.057 -0.412
ENSG00000025796 E029 181.9795827 0.0052233878 2.127953e-03 7.621262e-03 6 107913366 107913427 62 - 2.255 2.145 -0.370
ENSG00000025796 E030 227.8533258 0.0002140897 3.999963e-11 7.541611e-10 6 107921797 107921909 113 - 2.363 2.213 -0.502
ENSG00000025796 E031 215.9601249 0.0009008016 6.768302e-15 2.290870e-13 6 107924818 107924932 115 - 2.355 2.138 -0.724
ENSG00000025796 E032 195.4614031 0.0011533424 2.378932e-17 1.104070e-15 6 107929415 107929514 100 - 2.321 2.059 -0.877
ENSG00000025796 E033 0.8772771 0.6813704543 7.471625e-01 8.347384e-01 6 107954584 107954766 183 - 0.250 0.306 0.398
ENSG00000025796 E034 3.7267650 0.0041869322 2.580753e-01 3.952753e-01 6 107955965 107956106 142 - 0.573 0.768 0.827
ENSG00000025796 E035 0.4460135 0.0346837996 3.581200e-01 5.024987e-01 6 107957222 107957340 119 - 0.201 0.000 -11.094
ENSG00000025796 E036 187.5445057 0.0038850627 2.219481e-11 4.370832e-10 6 107957886 107958208 323 - 2.304 2.041 -0.879