ENSG00000025293

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374012 ENSG00000025293 HEK293_OSMI2_2hA HEK293_TMG_2hB PHF20 protein_coding protein_coding 19.90482 17.03968 26.11571 1.509761 1.265027 0.6157251 7.874177 3.580488475 11.472358 0.214325119 0.1180695 1.6771669 0.37335833 0.2128000 0.44100000 0.22820000 1.537134e-07 1.537134e-07 FALSE TRUE
ENST00000617560 ENSG00000025293 HEK293_OSMI2_2hA HEK293_TMG_2hB PHF20 protein_coding processed_transcript 19.90482 17.03968 26.11571 1.509761 1.265027 0.6157251 7.171540 8.394385513 7.567474 0.978344739 0.6756717 -0.1494254 0.38158333 0.4911000 0.28886667 -0.20223333 5.193774e-06 1.537134e-07   FALSE
MSTRG.20640.2 ENSG00000025293 HEK293_OSMI2_2hA HEK293_TMG_2hB PHF20 protein_coding   19.90482 17.03968 26.11571 1.509761 1.265027 0.6157251 1.022802 0.006169066 1.511317 0.006169066 0.3720042 6.5559406 0.04044167 0.0003000 0.05916667 0.05886667 4.983688e-07 1.537134e-07 FALSE TRUE
MSTRG.20640.3 ENSG00000025293 HEK293_OSMI2_2hA HEK293_TMG_2hB PHF20 protein_coding   19.90482 17.03968 26.11571 1.509761 1.265027 0.6157251 2.380783 3.701820911 2.859930 0.689088923 0.6935268 -0.3711117 0.13088750 0.2167667 0.10833333 -0.10843333 8.900094e-02 1.537134e-07   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000025293 E001 0.1472490 0.0428913923 1.000000e+00   20 35771974 35771989 16 + 0.074 0.000 -9.453
ENSG00000025293 E002 0.1472490 0.0428913923 1.000000e+00   20 35771990 35771995 6 + 0.074 0.000 -11.572
ENSG00000025293 E003 0.2924217 0.0290785164 6.779299e-01   20 35771996 35771997 2 + 0.137 0.000 -12.706
ENSG00000025293 E004 1.7212434 0.0123491487 9.368327e-01 9.642904e-01 20 35771998 35772008 11 + 0.394 0.434 0.214
ENSG00000025293 E005 3.6388392 0.0045275625 6.584812e-01 7.676032e-01 20 35772009 35772011 3 + 0.598 0.700 0.436
ENSG00000025293 E006 3.9343814 0.0042077518 5.299394e-01 6.626603e-01 20 35772012 35772014 3 + 0.618 0.746 0.541
ENSG00000025293 E007 6.3388868 0.0027047723 4.871351e-02 1.068784e-01 20 35772015 35772021 7 + 0.721 1.007 1.105
ENSG00000025293 E008 13.7151172 0.0012787706 2.266370e-01 3.583742e-01 20 35772022 35772025 4 + 1.076 1.231 0.553
ENSG00000025293 E009 18.2566811 0.0009929311 6.026222e-01 7.235661e-01 20 35772026 35772032 7 + 1.214 1.299 0.298
ENSG00000025293 E010 75.9873323 0.0013179828 8.691927e-06 5.856455e-05 20 35772033 35772079 47 + 1.761 2.000 0.804
ENSG00000025293 E011 98.5135586 0.0070507812 1.825464e-26 2.258842e-24 20 35774488 35775058 571 + 1.688 2.293 2.031
ENSG00000025293 E012 2.3281312 0.0065982612 3.379840e-03 1.137827e-02 20 35782328 35782609 282 + 0.285 0.789 2.476
ENSG00000025293 E013 5.1691128 0.0031344863 1.539163e-03 5.763443e-03 20 35800094 35800173 80 + 0.577 1.030 1.808
ENSG00000025293 E014 86.7079095 0.0038737155 2.479590e-01 3.835171e-01 20 35801491 35801605 115 + 1.868 1.964 0.324
ENSG00000025293 E015 0.0000000       20 35801606 35801972 367 +      
ENSG00000025293 E016 7.8931375 0.0481801164 2.480742e-02 6.147873e-02 20 35812623 35812719 97 + 0.778 1.145 1.377
ENSG00000025293 E017 19.7081769 0.0305977621 3.397216e-09 4.563514e-08 20 35823258 35823477 220 + 0.934 1.645 2.507
ENSG00000025293 E018 0.0000000       20 35838355 35838442 88 +      
ENSG00000025293 E019 78.9964129 0.0043006247 9.408647e-06 6.292371e-05 20 35842573 35842744 172 + 1.907 1.692 -0.728
ENSG00000025293 E020 0.4439371 0.0215666601 4.015789e-01 5.452838e-01 20 35845385 35845508 124 + 0.192 0.000 -13.376
ENSG00000025293 E021 44.8017366 0.0043278566 1.819093e-04 8.861059e-04 20 35847350 35847434 85 + 1.669 1.445 -0.765
ENSG00000025293 E022 0.0000000       20 35849389 35849483 95 +      
ENSG00000025293 E023 0.1482932 0.0410220055 1.674128e-01   20 35855231 35855253 23 + 0.000 0.196 12.769
ENSG00000025293 E024 0.1482932 0.0410220055 1.674128e-01   20 35855254 35855291 38 + 0.000 0.196 12.769
ENSG00000025293 E025 0.2214452 0.0393715861 1.653612e-01   20 35855301 35855572 272 + 0.000 0.197 12.792
ENSG00000025293 E026 0.2214452 0.0393715861 1.653612e-01   20 35856356 35856530 175 + 0.000 0.197 12.792
ENSG00000025293 E027 43.6922308 0.0004797123 1.055539e-05 6.978495e-05 20 35858302 35858381 80 + 1.661 1.418 -0.831
ENSG00000025293 E028 120.2259369 0.0003056271 1.150581e-17 5.602009e-16 20 35863013 35863400 388 + 2.105 1.802 -1.017
ENSG00000025293 E029 60.1442447 0.0005796994 4.514670e-09 5.932927e-08 20 35869438 35869529 92 + 1.804 1.512 -0.995
ENSG00000025293 E030 40.3516716 0.0005363517 1.334877e-04 6.744233e-04 20 35869530 35869551 22 + 1.620 1.408 -0.727
ENSG00000025293 E031 77.0213643 0.0008075363 1.273881e-07 1.268105e-06 20 35870955 35871134 180 + 1.897 1.675 -0.749
ENSG00000025293 E032 67.7626869 0.0094537150 1.741103e-02 4.584579e-02 20 35871650 35871829 180 + 1.822 1.698 -0.419
ENSG00000025293 E033 0.0000000       20 35871830 35871893 64 +      
ENSG00000025293 E034 67.1315561 0.0114691217 2.778644e-01 4.174912e-01 20 35899370 35899648 279 + 1.799 1.758 -0.139
ENSG00000025293 E035 47.9166359 0.0046127453 1.442865e-02 3.919679e-02 20 35913249 35913347 99 + 1.678 1.555 -0.419
ENSG00000025293 E036 69.1051082 0.0003486437 6.018006e-04 2.539975e-03 20 35914033 35914197 165 + 1.833 1.706 -0.429
ENSG00000025293 E037 0.1451727 0.0428647692 1.000000e+00   20 35917180 35917387 208 + 0.074 0.000 -11.572
ENSG00000025293 E038 86.7531757 0.0003555074 1.184725e-02 3.316723e-02 20 35917484 35917662 179 + 1.916 1.850 -0.223
ENSG00000025293 E039 60.7826329 0.0072522823 2.596633e-02 6.387714e-02 20 35927780 35927879 100 + 1.773 1.665 -0.366
ENSG00000025293 E040 71.8767046 0.0004571117 2.733627e-02 6.668008e-02 20 35931249 35931444 196 + 1.832 1.769 -0.213
ENSG00000025293 E041 0.0000000       20 35938632 35938696 65 +      
ENSG00000025293 E042 108.3907259 0.0022490173 3.194315e-02 7.574642e-02 20 35938697 35939108 412 + 2.009 1.957 -0.173
ENSG00000025293 E043 64.3552450 0.0044157277 1.050641e-02 2.999364e-02 20 35940864 35941047 184 + 1.800 1.690 -0.374
ENSG00000025293 E044 613.7261302 0.0019451816 1.209623e-09 1.764530e-08 20 35947485 35950370 2886 + 2.692 2.854 0.539