ENSG00000025156

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368455 ENSG00000025156 HEK293_OSMI2_2hA HEK293_TMG_2hB HSF2 protein_coding protein_coding 19.69188 8.897713 28.80343 1.087312 0.1801162 1.693614 8.645441 4.031378 13.40081 0.1992862 0.3133435 1.730478 0.4407458 0.4698667 0.4652667 -0.0046000 1.000000e+00 3.839053e-15 FALSE TRUE
ENST00000452194 ENSG00000025156 HEK293_OSMI2_2hA HEK293_TMG_2hB HSF2 protein_coding protein_coding 19.69188 8.897713 28.80343 1.087312 0.1801162 1.693614 9.936998 1.459014 15.40262 0.2165256 0.3214239 3.391191 0.4261833 0.1674667 0.5347333 0.3672667 6.239845e-11 3.839053e-15 FALSE TRUE
ENST00000465214 ENSG00000025156 HEK293_OSMI2_2hA HEK293_TMG_2hB HSF2 protein_coding protein_coding 19.69188 8.897713 28.80343 1.087312 0.1801162 1.693614 1.109444 3.407320 0.00000 1.2427071 0.0000000 -8.416722 0.1330625 0.3626667 0.0000000 -0.3626667 3.839053e-15 3.839053e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000025156 E001 5.31628 0.1000893016 4.830168e-01 0.6211087151 6 122399551 122399651 101 + 0.764 0.634 -0.540
ENSG00000025156 E002 95.27044 0.0023597907 9.600005e-05 0.0005032770 6 122399652 122399830 179 + 1.939 1.793 -0.491
ENSG00000025156 E003 115.59347 0.0301997484 1.168834e-02 0.0327926019 6 122412373 122412481 109 + 2.027 1.849 -0.599
ENSG00000025156 E004 117.07689 0.0008523480 3.052481e-05 0.0001813028 6 122412637 122412764 128 + 2.023 1.899 -0.416
ENSG00000025156 E005 115.00396 0.0003236099 9.999300e-02 0.1904813852 6 122413525 122413649 125 + 1.992 1.981 -0.037
ENSG00000025156 E006 108.52836 0.0002771924 8.155832e-01 0.8832512570 6 122416221 122416296 76 + 1.954 1.999 0.150
ENSG00000025156 E007 102.98792 0.0003269043 4.660574e-01 0.6056271972 6 122419168 122419229 62 + 1.936 1.960 0.080
ENSG00000025156 E008 144.76803 0.0002590557 3.392519e-02 0.0796024930 6 122420135 122420222 88 + 2.093 2.072 -0.068
ENSG00000025156 E009 81.54262 0.0006354051 2.777153e-04 0.0012888862 6 122422150 122422155 6 + 1.868 1.740 -0.430
ENSG00000025156 E010 189.34111 0.0002696134 4.697714e-04 0.0020445398 6 122422156 122422298 143 + 2.216 2.162 -0.182
ENSG00000025156 E011 249.34347 0.0003241628 1.369746e-01 0.2438718597 6 122422718 122422957 240 + 2.319 2.334 0.049
ENSG00000025156 E012 156.68441 0.0002493918 9.080501e-01 0.9456324433 6 122423581 122423686 106 + 2.108 2.166 0.192
ENSG00000025156 E013 44.68130 0.0280138769 6.561269e-01 0.7658262832 6 122427903 122427956 54 + 1.561 1.653 0.315
ENSG00000025156 E014 145.09895 0.0002884886 9.439289e-01 0.9688446484 6 122431430 122431514 85 + 2.078 2.129 0.172
ENSG00000025156 E015 508.09180 0.0114902196 3.104736e-06 0.0000231486 6 122431925 122433119 1195 + 2.559 2.815 0.854