ENSG00000024422

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263277 ENSG00000024422 HEK293_OSMI2_2hA HEK293_TMG_2hB EHD2 protein_coding protein_coding 1.079105 2.06027 0.8979689 0.164082 0.06399623 -1.189104 0.93976086 1.4077908 0.85549845 0.2457109 0.03701876 -0.7120415 0.9249792 0.6728333 0.95773333 0.2849 0.03616753 0.03616753 FALSE TRUE
ENST00000540884 ENSG00000024422 HEK293_OSMI2_2hA HEK293_TMG_2hB EHD2 protein_coding processed_transcript 1.079105 2.06027 0.8979689 0.164082 0.06399623 -1.189104 0.08932589 0.3745008 0.04247041 0.1873129 0.04247041 -2.8734108 0.0448375 0.1704667 0.04226667 -0.1282 0.65479783 0.03616753 FALSE TRUE
ENST00000596225 ENSG00000024422 HEK293_OSMI2_2hA HEK293_TMG_2hB EHD2 protein_coding nonsense_mediated_decay 1.079105 2.06027 0.8979689 0.164082 0.06399623 -1.189104 0.03474729 0.2779784 0.00000000 0.2779784 0.00000000 -4.8478885 0.0195875 0.1567000 0.00000000 -0.1567 0.90691302 0.03616753 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000024422 E001 5.8208752 0.0032597985 0.340566609 0.48451388 19 47713422 47713538 117 + 0.869 0.742 -0.502
ENSG00000024422 E002 20.4122293 0.0095807387 0.185307977 0.30788291 19 47716558 47717016 459 + 1.346 1.233 -0.396
ENSG00000024422 E003 9.2412944 0.0019711479 0.954715423 0.97561856 19 47718509 47718606 98 + 0.948 0.952 0.015
ENSG00000024422 E004 14.2642775 0.0013659387 0.172555557 0.29151959 19 47725812 47726168 357 + 1.211 1.086 -0.448
ENSG00000024422 E005 5.2200234 0.0033169843 0.640332435 0.75359411 19 47726169 47726203 35 + 0.694 0.758 0.265
ENSG00000024422 E006 5.2284570 0.0033335690 0.836099409 0.89750988 19 47726204 47726224 21 + 0.773 0.742 -0.125
ENSG00000024422 E007 0.6298062 0.0198413837 0.819619254 0.88610255 19 47726225 47726319 95 + 0.174 0.216 0.386
ENSG00000024422 E008 0.1515154 0.0419815695 0.213169941   19 47729617 47729681 65 + 0.174 0.000 -10.554
ENSG00000024422 E009 0.0000000       19 47731331 47731508 178 +      
ENSG00000024422 E010 10.9929337 0.0028737357 0.087090591 0.17073089 19 47736369 47736533 165 + 1.155 0.982 -0.630
ENSG00000024422 E011 98.1851346 0.0006503968 0.003130872 0.01065064 19 47740881 47743134 2254 + 1.880 1.958 0.262