ENSG00000023516

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000025301 ENSG00000023516 HEK293_OSMI2_2hA HEK293_TMG_2hB AKAP11 protein_coding protein_coding 3.99142 0.9949671 6.181559 0.03925989 0.1960044 2.623155 1.478261 0.1557025 2.108791 0.08629448 0.1906023 3.6765738 0.3366583 0.1591667 0.3436667 0.1845000 0.2322623847 0.0001577414 FALSE TRUE
MSTRG.8665.1 ENSG00000023516 HEK293_OSMI2_2hA HEK293_TMG_2hB AKAP11 protein_coding   3.99142 0.9949671 6.181559 0.03925989 0.1960044 2.623155 1.407231 0.1022451 2.596716 0.06676303 0.2170330 4.5375095 0.2409667 0.1052667 0.4191667 0.3139000 0.0039158601 0.0001577414 FALSE TRUE
MSTRG.8665.2 ENSG00000023516 HEK293_OSMI2_2hA HEK293_TMG_2hB AKAP11 protein_coding   3.99142 0.9949671 6.181559 0.03925989 0.1960044 2.623155 1.105928 0.7370195 1.476052 0.09884819 0.2702883 0.9922669 0.4223708 0.7355333 0.2372000 -0.4983333 0.0001577414 0.0001577414 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000023516 E001 1.627354 0.0084811327 5.795832e-01 7.044569e-01 13 42272133 42272151 19 + 0.306 0.309 0.019
ENSG00000023516 E002 10.313412 0.0166919406 1.779877e-01 2.986091e-01 13 42272152 42272228 77 + 0.865 0.859 -0.022
ENSG00000023516 E003 8.779184 0.0020741218 1.608389e-01 2.762155e-01 13 42285986 42286035 50 + 0.807 0.791 -0.064
ENSG00000023516 E004 16.272102 0.0010872480 1.915588e-02 4.967364e-02 13 42286300 42286399 100 + 1.050 0.968 -0.302
ENSG00000023516 E005 18.339129 0.0009619481 4.382627e-04 1.921712e-03 13 42292385 42292501 117 + 1.113 0.858 -0.944
ENSG00000023516 E006 12.846201 0.0013996621 2.588973e-03 9.032157e-03 13 42295695 42295742 48 + 0.972 0.711 -1.016
ENSG00000023516 E007 10.589744 0.0017413883 8.019478e-05 4.287904e-04 13 42297048 42297182 135 + 0.911 0.309 -2.787
ENSG00000023516 E008 31.517398 0.0552887798 2.738722e-03 9.489058e-03 13 42298533 42298797 265 + 1.331 1.060 -0.964
ENSG00000023516 E009 388.378248 0.0418482481 7.116008e-08 7.433317e-07 13 42299363 42303863 4501 + 2.397 2.221 -0.586
ENSG00000023516 E010 37.076173 0.0385851477 1.876386e-01 3.107658e-01 13 42308454 42308609 156 + 1.365 1.441 0.261
ENSG00000023516 E011 11.346724 0.0016778364 7.593544e-02 1.530091e-01 13 42313047 42313130 84 + 0.906 0.858 -0.182
ENSG00000023516 E012 26.610568 0.0216894578 3.693712e-01 5.137985e-01 13 42313894 42313940 47 + 1.222 1.342 0.422
ENSG00000023516 E013 32.997889 0.0130382323 5.781434e-01 7.032988e-01 13 42317528 42317688 161 + 1.306 1.479 0.598
ENSG00000023516 E014 378.840114 0.0694662638 5.097472e-07 4.502522e-06 13 42319088 42323261 4174 + 2.258 2.837 1.930