ENSG00000023171

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000533341 ENSG00000023171 HEK293_OSMI2_2hA HEK293_TMG_2hB GRAMD1B protein_coding protein_coding 1.849309 2.388893 1.479756 0.2412706 0.1057506 -0.6872924 0.51388781 0.8605677 0.3340887 0.08063718 0.01990319 -1.33917611 0.2611667 0.3616333 0.2264333 -0.1352000 0.108674998 0.003641887 FALSE TRUE
ENST00000635736 ENSG00000023171 HEK293_OSMI2_2hA HEK293_TMG_2hB GRAMD1B protein_coding protein_coding 1.849309 2.388893 1.479756 0.2412706 0.1057506 -0.6872924 1.05582212 1.1227828 1.1008687 0.02506613 0.10132247 -0.02818306 0.5996583 0.4779667 0.7417333 0.2637667 0.003641887 0.003641887 FALSE TRUE
ENST00000640939 ENSG00000023171 HEK293_OSMI2_2hA HEK293_TMG_2hB GRAMD1B protein_coding protein_coding 1.849309 2.388893 1.479756 0.2412706 0.1057506 -0.6872924 0.07970292 0.2173039 0.0000000 0.09863372 0.00000000 -4.50655078 0.0407750 0.0928000 0.0000000 -0.0928000 0.013133764 0.003641887 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000023171 E001 0.1817044 0.038835057 5.822449e-01   11 123358428 123358799 372 + 0.000 0.116 9.029
ENSG00000023171 E002 4.2353570 0.004367467 9.177893e-01 9.520318e-01 11 123430269 123430349 81 + 0.683 0.722 0.159
ENSG00000023171 E003 35.2212628 0.001052093 9.762988e-02 1.868704e-01 11 123430350 123431166 817 + 1.470 1.596 0.431
ENSG00000023171 E004 1.5457760 0.017243524 4.104489e-03 1.343646e-02 11 123432018 123432126 109 + 0.000 0.537 14.100
ENSG00000023171 E005 12.0755237 0.001801766 1.226847e-05 7.985108e-05 11 123435434 123435719 286 + 0.768 1.257 1.810
ENSG00000023171 E006 0.0000000       11 123454398 123454657 260 +      
ENSG00000023171 E007 0.1482932 0.041751290 5.825725e-01   11 123454658 123454829 172 + 0.000 0.115 11.310
ENSG00000023171 E008 0.0000000       11 123458372 123460410 2039 +      
ENSG00000023171 E009 0.0000000       11 123480738 123480815 78 +      
ENSG00000023171 E010 4.8674339 0.003341965 3.893336e-03 1.284514e-02 11 123480816 123480893 78 + 0.937 0.573 -1.479
ENSG00000023171 E011 0.0000000       11 123491653 123491959 307 +      
ENSG00000023171 E012 0.3634088 0.250316928 3.256490e-01   11 123509918 123510037 120 + 0.000 0.208 11.794
ENSG00000023171 E013 0.0000000       11 123525636 123525821 186 +      
ENSG00000023171 E014 0.0000000       11 123525822 123526171 350 +      
ENSG00000023171 E015 0.0000000       11 123560262 123560276 15 +      
ENSG00000023171 E016 0.0000000       11 123560277 123560527 251 +      
ENSG00000023171 E017 0.0000000       11 123560528 123560652 125 +      
ENSG00000023171 E018 8.6311910 0.002580481 2.627651e-01 4.005687e-01 11 123577367 123577577 211 + 1.031 0.935 -0.353
ENSG00000023171 E019 0.0000000       11 123578410 123578430 21 +      
ENSG00000023171 E020 3.4841120 0.005050120 8.153843e-01 8.831015e-01 11 123584312 123584332 21 + 0.651 0.639 -0.051
ENSG00000023171 E021 0.1472490 0.044278127 4.061466e-01   11 123591087 123591541 455 + 0.130 0.000 -12.376
ENSG00000023171 E022 6.4014533 0.003055844 5.452265e-01 6.756158e-01 11 123594082 123594166 85 + 0.881 0.832 -0.188
ENSG00000023171 E023 7.5460740 0.002457293 8.996402e-02 1.750844e-01 11 123594735 123594838 104 + 1.016 0.851 -0.623
ENSG00000023171 E024 7.2911169 0.070739457 1.692818e-01 2.872509e-01 11 123595942 123596037 96 + 1.016 0.829 -0.707
ENSG00000023171 E025 6.6654848 0.002890995 8.919481e-01 9.349997e-01 11 123600468 123600548 81 + 0.861 0.903 0.163
ENSG00000023171 E026 9.2086828 0.094872183 5.529464e-01 6.821656e-01 11 123603426 123603541 116 + 1.016 0.988 -0.102
ENSG00000023171 E027 9.2738846 0.017189974 1.688911e-01 2.867352e-01 11 123605322 123605478 157 + 1.071 0.938 -0.490
ENSG00000023171 E028 12.7111500 0.002144126 1.948301e-02 5.037735e-02 11 123606609 123606798 190 + 1.230 1.044 -0.669
ENSG00000023171 E029 0.5149111 0.021798013 6.863217e-01 7.888708e-01 11 123607809 123608658 850 + 0.130 0.207 0.811
ENSG00000023171 E030 11.0243133 0.007312869 6.270092e-03 1.931998e-02 11 123608659 123608802 144 + 1.203 0.951 -0.915
ENSG00000023171 E031 10.5832552 0.003245958 1.616474e-01 2.772642e-01 11 123609795 123609913 119 + 1.119 1.006 -0.411
ENSG00000023171 E032 10.6326628 0.007599837 4.080447e-02 9.252065e-02 11 123610196 123610338 143 + 1.152 0.966 -0.679
ENSG00000023171 E033 8.7148384 0.002114681 2.593214e-01 3.967026e-01 11 123612761 123612864 104 + 1.031 0.936 -0.353
ENSG00000023171 E034 0.5181333 0.021245618 4.604752e-01 6.005153e-01 11 123613125 123613454 330 + 0.229 0.116 -1.188
ENSG00000023171 E035 11.7008898 0.001778410 5.476272e-01 6.776509e-01 11 123613455 123613658 204 + 1.119 1.089 -0.110
ENSG00000023171 E036 8.1577072 0.068708946 6.776100e-01 7.822837e-01 11 123614745 123614835 91 + 0.985 0.955 -0.114
ENSG00000023171 E037 0.0000000       11 123618345 123618356 12 +      
ENSG00000023171 E038 9.2243663 0.002463602 1.258090e-01 2.282986e-01 11 123618693 123618800 108 + 1.084 0.950 -0.490
ENSG00000023171 E039 11.1891511 0.082964728 4.456447e-01 5.872013e-01 11 123619107 123619224 118 + 1.140 1.026 -0.413
ENSG00000023171 E040 0.0000000       11 123619225 123619481 257 +      
ENSG00000023171 E041 9.6801641 0.014739565 6.679068e-01 7.750490e-01 11 123622506 123622638 133 + 0.970 1.046 0.278
ENSG00000023171 E042 123.2450405 0.010360895 8.466219e-01 9.046766e-01 11 123622639 123626593 3955 + 2.076 2.095 0.064
ENSG00000023171 E043 62.2047470 0.024627022 2.862689e-04 1.324010e-03 11 123626594 123627774 1181 + 1.582 1.907 1.099