ENSG00000018869

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000301310 ENSG00000018869 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF582 protein_coding protein_coding 2.684106 3.125739 3.471393 0.7391767 0.4602246 0.1508592 0.07047961 0.1505628 0.0000000 0.15056280 0.00000000 -4.0050658 0.03273750 0.08090000 0.0000000 -0.08090000 0.8193601854 0.0004683048 FALSE TRUE
ENST00000586929 ENSG00000018869 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF582 protein_coding protein_coding 2.684106 3.125739 3.471393 0.7391767 0.4602246 0.1508592 0.60986578 0.1215358 1.0203309 0.06967219 0.12108187 2.9695809 0.23290000 0.03146667 0.3119000 0.28043333 0.0004683048 0.0004683048 FALSE TRUE
ENST00000587778 ENSG00000018869 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF582 protein_coding non_stop_decay 2.684106 3.125739 3.471393 0.7391767 0.4602246 0.1508592 0.27664073 0.0000000 0.4490719 0.00000000 0.44907187 5.5206481 0.10317917 0.00000000 0.1572667 0.15726667 0.8815151600 0.0004683048 FALSE FALSE
ENST00000589143 ENSG00000018869 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF582 protein_coding protein_coding 2.684106 3.125739 3.471393 0.7391767 0.4602246 0.1508592 0.39246020 0.5052851 0.3401463 0.28678326 0.07573433 -0.5574132 0.15171250 0.13116667 0.0984000 -0.03276667 0.9665673584 0.0004683048 FALSE TRUE
ENST00000589895 ENSG00000018869 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF582 protein_coding protein_coding 2.684106 3.125739 3.471393 0.7391767 0.4602246 0.1508592 0.04629133 0.1270613 0.0000000 0.07018989 0.00000000 -3.7767489 0.01734583 0.05386667 0.0000000 -0.05386667 0.1780993524 0.0004683048 FALSE FALSE
ENST00000593145 ENSG00000018869 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF582 protein_coding non_stop_decay 2.684106 3.125739 3.471393 0.7391767 0.4602246 0.1508592 0.55540286 0.9387307 0.6797354 0.46974338 0.34469365 -0.4599556 0.18290000 0.25673333 0.1815667 -0.07516667 0.9419261464 0.0004683048 FALSE FALSE
MSTRG.17764.2 ENSG00000018869 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF582 protein_coding   2.684106 3.125739 3.471393 0.7391767 0.4602246 0.1508592 0.73296549 1.2825632 0.9821083 0.16440501 0.58432671 -0.3816652 0.27922500 0.44583333 0.2508667 -0.19496667 0.6652527218 0.0004683048 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000018869 E001 2.612990 0.012389577 0.10738534 0.20168566 19 56375846 56376001 156 - 0.459 0.672 0.975
ENSG00000018869 E002 6.234133 0.002866728 0.03159260 0.07505891 19 56376002 56376118 117 - 0.756 0.959 0.784
ENSG00000018869 E003 4.025237 0.004608279 0.11944186 0.21914795 19 56376119 56376121 3 - 0.616 0.783 0.691
ENSG00000018869 E004 0.000000       19 56382751 56382751 1 -      
ENSG00000018869 E005 34.532474 0.111611661 0.01358374 0.03726838 19 56382752 56384872 2121 - 1.725 1.292 -1.486
ENSG00000018869 E006 2.028427 0.007217928 0.11462366 0.21228180 19 56384873 56384881 9 - 0.616 0.304 -1.628
ENSG00000018869 E007 14.811408 0.003018183 0.89010958 0.93379068 19 56384882 56385184 303 - 1.246 1.171 -0.265
ENSG00000018869 E008 19.741399 0.007826398 0.03044292 0.07283166 19 56390001 56390096 96 - 1.268 1.376 0.377
ENSG00000018869 E009 23.287946 0.002322962 0.15910797 0.27392088 19 56390375 56390501 127 - 1.378 1.408 0.105
ENSG00000018869 E010 19.288729 0.001091283 0.09232948 0.17875875 19 56391744 56391832 89 - 1.283 1.341 0.203
ENSG00000018869 E011 9.710209 0.010028525 0.49265973 0.62978989 19 56393220 56393236 17 - 1.100 0.959 -0.516
ENSG00000018869 E012 17.500607 0.001203144 0.96625377 0.98281323 19 56393237 56393441 205 - 1.310 1.244 -0.232
ENSG00000018869 E013 9.642239 0.010807230 0.16933976 0.28732688 19 56393442 56393538 97 - 0.990 1.082 0.334