ENSG00000018610

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000644802 ENSG00000018610 HEK293_OSMI2_2hA HEK293_TMG_2hB STEEP1 protein_coding protein_coding 39.50796 36.95676 48.2799 6.581706 1.901648 0.3854927 32.901756 34.74807 35.36001 6.716439 1.0783159 0.02517883 0.8453583 0.9350667 0.7329333 -0.2021333 1.480343e-07 1.922086e-69 FALSE TRUE
MSTRG.34787.4 ENSG00000018610 HEK293_OSMI2_2hA HEK293_TMG_2hB STEEP1 protein_coding   39.50796 36.95676 48.2799 6.581706 1.901648 0.3854927 4.547667 0.00000 10.16367 0.000000 0.1905089 9.99062401 0.1013750 0.0000000 0.2111000 0.2111000 1.922086e-69 1.922086e-69 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000018610 E001 1001.100734 0.0005973456 3.015991e-15 1.072996e-13 X 119538149 119539317 1169 - 2.941 3.057 0.385
ENSG00000018610 E002 181.148287 0.0002600282 5.837598e-01 7.078039e-01 X 119539318 119539532 215 - 2.252 2.280 0.094
ENSG00000018610 E003 132.337254 0.0003541525 4.305879e-02 9.665692e-02 X 119539533 119539569 37 - 2.159 2.111 -0.161
ENSG00000018610 E004 459.612634 0.0016205875 1.911222e-01 3.151452e-01 X 119539570 119539789 220 - 2.649 2.683 0.114
ENSG00000018610 E005 414.212730 0.0011896075 9.157640e-01 9.507222e-01 X 119541328 119541420 93 - 2.621 2.623 0.008
ENSG00000018610 E006 405.925348 0.0001709929 2.118616e-04 1.014676e-03 X 119542505 119542594 90 - 2.643 2.593 -0.167
ENSG00000018610 E007 3.947257 0.0112348735 7.208123e-04 2.973321e-03 X 119542595 119542767 173 - 0.888 0.367 -2.343
ENSG00000018610 E008 408.179782 0.0001705710 4.808029e-05 2.720792e-04 X 119544353 119544491 139 - 2.649 2.594 -0.185
ENSG00000018610 E009 239.548025 0.0006628734 3.153058e-02 7.493976e-02 X 119545463 119545504 42 - 2.410 2.372 -0.129
ENSG00000018610 E010 0.147249 0.0460910169 5.128019e-01   X 119560178 119560267 90 - 0.121 0.000 -8.979
ENSG00000018610 E011 338.269902 0.0001745224 6.904944e-03 2.097827e-02 X 119560268 119560385 118 - 2.558 2.521 -0.123
ENSG00000018610 E012 2.097124 0.0181057866 5.808479e-01 7.055286e-01 X 119565125 119565231 107 - 0.552 0.473 -0.376
ENSG00000018610 E013 283.700851 0.0007193792 5.881921e-04 2.490417e-03 X 119565232 119565409 178 - 2.496 2.425 -0.235