ENSG00000018189

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000226328 ENSG00000018189 HEK293_OSMI2_2hA HEK293_TMG_2hB RUFY3 protein_coding protein_coding 7.576355 4.554447 11.47355 0.321669 0.523108 1.331057 1.1210557 0.45068419 1.7120600 0.009886078 0.07075293 1.9022854 0.13712083 0.10003333 0.14956667 0.04953333 0.17821270 0.00504535 FALSE TRUE
ENST00000417478 ENSG00000018189 HEK293_OSMI2_2hA HEK293_TMG_2hB RUFY3 protein_coding protein_coding 7.576355 4.554447 11.47355 0.321669 0.523108 1.331057 1.5050827 0.43864956 2.7423483 0.124062715 0.14921361 2.6170021 0.17337500 0.10086667 0.23920000 0.13833333 0.05512000 0.00504535 FALSE TRUE
ENST00000502653 ENSG00000018189 HEK293_OSMI2_2hA HEK293_TMG_2hB RUFY3 protein_coding protein_coding 7.576355 4.554447 11.47355 0.321669 0.523108 1.331057 1.9155942 1.80678075 2.6045877 0.081935944 0.57513836 0.5251991 0.26776667 0.39976667 0.23030000 -0.16946667 0.16908340 0.00504535 FALSE TRUE
ENST00000503025 ENSG00000018189 HEK293_OSMI2_2hA HEK293_TMG_2hB RUFY3 protein_coding retained_intron 7.576355 4.554447 11.47355 0.321669 0.523108 1.331057 0.3909551 0.07831155 0.6067506 0.078311547 0.08079617 2.8040132 0.05231667 0.01816667 0.05260000 0.03443333 0.29600277 0.00504535 FALSE FALSE
ENST00000512103 ENSG00000018189 HEK293_OSMI2_2hA HEK293_TMG_2hB RUFY3 protein_coding nonsense_mediated_decay 7.576355 4.554447 11.47355 0.321669 0.523108 1.331057 0.2934974 0.55212360 0.1610602 0.037078276 0.11207584 -1.7163834 0.05047083 0.12170000 0.01326667 -0.10843333 0.00504535 0.00504535   FALSE
ENST00000512331 ENSG00000018189 HEK293_OSMI2_2hA HEK293_TMG_2hB RUFY3 protein_coding processed_transcript 7.576355 4.554447 11.47355 0.321669 0.523108 1.331057 0.4464188 0.41749725 0.5996431 0.172200660 0.14266720 0.5120497 0.06230417 0.08810000 0.05266667 -0.03543333 0.66544308 0.00504535   FALSE
MSTRG.25085.10 ENSG00000018189 HEK293_OSMI2_2hA HEK293_TMG_2hB RUFY3 protein_coding   7.576355 4.554447 11.47355 0.321669 0.523108 1.331057 0.6047902 0.40382212 1.4448614 0.120657409 0.48661018 1.8137990 0.07721667 0.08633333 0.12286667 0.03653333 0.76611039 0.00504535 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000018189 E001 0.0000000       4 70703764 70703984 221 +      
ENSG00000018189 E002 0.0000000       4 70703985 70703986 2 +      
ENSG00000018189 E003 0.0000000       4 70703987 70704203 217 +      
ENSG00000018189 E004 0.4449813 0.0212180566 9.594845e-01 0.9785654212 4 70704204 70704348 145 + 0.152 0.178 0.269
ENSG00000018189 E005 0.0000000       4 70704349 70704423 75 +      
ENSG00000018189 E006 0.1515154 0.0428940861 1.000000e+00   4 70704424 70704475 52 + 0.083 0.000 -9.598
ENSG00000018189 E007 2.5830814 0.0058827786 6.186407e-02 0.1297561352 4 70704476 70704800 325 + 0.617 0.303 -1.636
ENSG00000018189 E008 3.0376276 0.0050230277 2.519538e-02 0.0622725189 4 70704801 70704809 9 + 0.679 0.303 -1.899
ENSG00000018189 E009 6.0689256 0.0027247321 1.558515e-03 0.0058245081 4 70704810 70704893 84 + 0.932 0.480 -1.899
ENSG00000018189 E010 20.3884888 0.0010264073 1.291153e-04 0.0006549874 4 70704894 70705294 401 + 1.385 1.084 -1.064
ENSG00000018189 E011 1.1845624 0.0106457262 2.915701e-02 0.0703039041 4 70721964 70722010 47 + 0.427 0.000 -12.597
ENSG00000018189 E012 7.6268204 0.0025383618 4.824296e-01 0.6205661858 4 70722011 70722451 441 + 0.931 0.879 -0.198
ENSG00000018189 E013 12.9597791 0.0519794688 1.272624e-01 0.2303203445 4 70722452 70722751 300 + 1.178 0.982 -0.713
ENSG00000018189 E014 0.0000000       4 70734346 70734433 88 +      
ENSG00000018189 E015 0.1472490 0.0441366252 1.000000e+00   4 70734434 70734593 160 + 0.083 0.000 -9.597
ENSG00000018189 E016 0.7427016 0.0156045273 1.101190e-01 0.2057085093 4 70741627 70741680 54 + 0.311 0.000 -11.919
ENSG00000018189 E017 43.9277746 0.0120773973 1.849901e-02 0.0482292935 4 70762519 70762692 174 + 1.671 1.518 -0.523
ENSG00000018189 E018 41.9129057 0.0005182737 3.302092e-02 0.0778451445 4 70763552 70763669 118 + 1.635 1.549 -0.292
ENSG00000018189 E019 39.4163417 0.0005606346 7.561254e-02 0.1524863531 4 70764475 70764576 102 + 1.604 1.536 -0.231
ENSG00000018189 E020 55.6639078 0.0004108666 1.432377e-02 0.0389644194 4 70768538 70768661 124 + 1.757 1.672 -0.287
ENSG00000018189 E021 42.6421940 0.0016187873 3.609476e-02 0.0837687680 4 70773511 70773572 62 + 1.643 1.555 -0.300
ENSG00000018189 E022 45.7647232 0.0005732163 4.964089e-02 0.1085272259 4 70775168 70775233 66 + 1.671 1.601 -0.238
ENSG00000018189 E023 47.7607254 0.0005231788 3.097353e-01 0.4520713886 4 70778369 70778438 70 + 1.669 1.653 -0.054
ENSG00000018189 E024 54.4965795 0.0006613989 8.425634e-01 0.9019002864 4 70783091 70783183 93 + 1.701 1.749 0.163
ENSG00000018189 E025 50.1354337 0.0021199119 2.258264e-01 0.3573785094 4 70784796 70784865 70 + 1.643 1.746 0.348
ENSG00000018189 E026 32.2635918 0.0011859466 2.560122e-02 0.0631232385 4 70784866 70784879 14 + 1.428 1.607 0.614
ENSG00000018189 E027 60.4767081 0.0049462898 1.797086e-01 0.3007930153 4 70788806 70788938 133 + 1.721 1.832 0.376
ENSG00000018189 E028 29.5752257 0.0008246384 4.996890e-01 0.6359687287 4 70788939 70788973 35 + 1.428 1.510 0.283
ENSG00000018189 E029 41.5656639 0.0072201338 2.825564e-01 0.4225664962 4 70789495 70789592 98 + 1.566 1.672 0.362
ENSG00000018189 E030 20.2464757 0.0046244871 3.111919e-05 0.0001844976 4 70789593 70790082 490 + 1.392 1.026 -1.300
ENSG00000018189 E031 75.4700838 0.0004155337 3.055471e-05 0.0001814462 4 70791225 70793764 2540 + 1.903 1.757 -0.493
ENSG00000018189 E032 5.3398519 0.0035834064 1.412305e-01 0.2497264243 4 70793765 70793784 20 + 0.837 0.657 -0.730
ENSG00000018189 E033 31.5673285 0.0007915250 8.358252e-01 0.8973448143 4 70793785 70793875 91 + 1.485 1.510 0.085
ENSG00000018189 E034 26.3948892 0.0013968928 7.340308e-01 0.8251101493 4 70793876 70793904 29 + 1.414 1.428 0.048
ENSG00000018189 E035 3.0280507 0.0052293461 3.728828e-03 0.0123797834 4 70794673 70794794 122 + 0.697 0.178 -2.976
ENSG00000018189 E036 38.1534513 0.0006111814 7.342817e-01 0.8252794875 4 70794795 70794894 100 + 1.554 1.612 0.199
ENSG00000018189 E037 2.9643739 0.0057239772 2.624199e-01 0.4002050282 4 70799405 70799447 43 + 0.639 0.480 -0.731
ENSG00000018189 E038 9.1217378 0.1079308223 4.803119e-01 0.6186004013 4 70799448 70799875 428 + 1.019 0.921 -0.366
ENSG00000018189 E039 3.4751214 0.0078411480 1.075554e-02 0.0305799693 4 70799876 70800140 265 + 0.732 0.303 -2.121
ENSG00000018189 E040 34.9727865 0.0006131389 3.130991e-01 0.4556276323 4 70800141 70800205 65 + 1.497 1.596 0.339
ENSG00000018189 E041 1.3599240 0.0096319824 1.596410e-01 0.2746339457 4 70802871 70802955 85 + 0.427 0.178 -1.728
ENSG00000018189 E042 26.6662995 0.0007461311 6.873123e-02 0.1412630851 4 70802956 70802982 27 + 1.362 1.523 0.556
ENSG00000018189 E043 18.5434104 0.0010302938 3.160268e-01 0.4587905053 4 70802983 70802983 1 + 1.228 1.347 0.415
ENSG00000018189 E044 2.6036167 0.0522230296 7.552656e-01 0.8406596658 4 70802984 70803059 76 + 0.519 0.604 0.389
ENSG00000018189 E045 1.4027854 0.0095556457 5.527134e-01 0.6819535624 4 70803060 70803176 117 + 0.392 0.303 -0.536
ENSG00000018189 E046 26.2780289 0.0048120218 9.274753e-01 0.9583640208 4 70804348 70804416 69 + 1.399 1.443 0.152
ENSG00000018189 E047 293.0787237 0.0109351558 1.314494e-04 0.0006652003 4 70806516 70808626 2111 + 2.364 2.571 0.692