ENSG00000016864

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000266014 ENSG00000016864 HEK293_OSMI2_2hA HEK293_TMG_2hB GLT8D1 protein_coding protein_coding 39.62055 28.61844 42.67752 3.063741 0.9751933 0.5763655 6.862801 4.981348 9.818576 0.4280650 0.3963761 0.97755279 0.17430417 0.1801667 0.23056667 0.05040000 4.956785e-01 1.304861e-22 FALSE TRUE
ENST00000394783 ENSG00000016864 HEK293_OSMI2_2hA HEK293_TMG_2hB GLT8D1 protein_coding protein_coding 39.62055 28.61844 42.67752 3.063741 0.9751933 0.5763655 13.408259 10.431823 12.334418 2.2621988 0.6354527 0.24148522 0.33696667 0.3583667 0.28863333 -0.06973333 6.464641e-01 1.304861e-22 FALSE TRUE
ENST00000481643 ENSG00000016864 HEK293_OSMI2_2hA HEK293_TMG_2hB GLT8D1 protein_coding nonsense_mediated_decay 39.62055 28.61844 42.67752 3.063741 0.9751933 0.5763655 4.213042 4.225781 3.947205 1.8521404 0.6102100 -0.09814617 0.11155417 0.1405667 0.09196667 -0.04860000 7.492136e-01 1.304861e-22 FALSE TRUE
MSTRG.23064.24 ENSG00000016864 HEK293_OSMI2_2hA HEK293_TMG_2hB GLT8D1 protein_coding   39.62055 28.61844 42.67752 3.063741 0.9751933 0.5763655 2.647700 4.207374 2.179963 0.1287431 0.3914740 -0.94543804 0.07924167 0.1496333 0.05073333 -0.09890000 1.167720e-05 1.304861e-22   FALSE
MSTRG.23064.5 ENSG00000016864 HEK293_OSMI2_2hA HEK293_TMG_2hB GLT8D1 protein_coding   39.62055 28.61844 42.67752 3.063741 0.9751933 0.5763655 3.699790 0.000000 5.615348 0.0000000 0.8517146 9.13579860 0.07842917 0.0000000 0.13210000 0.13210000 1.304861e-22 1.304861e-22 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000016864 E001 3.3738610 0.0422364708 1.525725e-01 2.651731e-01 3 52694486 52694488 3 - 0.506 0.749 1.062
ENSG00000016864 E002 4.4723930 0.0041162333 3.307648e-03 1.116977e-02 3 52694489 52694491 3 - 0.506 0.918 1.724
ENSG00000016864 E003 6.0579254 0.0026663453 3.058605e-03 1.043835e-02 3 52694492 52694502 11 - 0.634 1.009 1.477
ENSG00000016864 E004 160.0085702 0.0015207639 1.611814e-04 7.972049e-04 3 52694503 52694671 169 - 2.146 2.272 0.421
ENSG00000016864 E005 261.5044959 0.0014247879 5.250500e-05 2.942787e-04 3 52694672 52694801 130 - 2.366 2.479 0.376
ENSG00000016864 E006 280.3599743 0.0002519404 1.425895e-01 2.515663e-01 3 52694802 52694898 97 - 2.438 2.469 0.105
ENSG00000016864 E007 286.3928452 0.0002422785 6.872119e-01 7.896012e-01 3 52694899 52695006 108 - 2.457 2.466 0.028
ENSG00000016864 E008 178.0900792 0.0002565005 2.956492e-01 4.367989e-01 3 52695007 52695035 29 - 2.243 2.271 0.093
ENSG00000016864 E009 23.4388628 0.0010036650 6.358221e-01 7.500006e-01 3 52695036 52695189 154 - 1.407 1.374 -0.117
ENSG00000016864 E010 206.5937148 0.0071264315 8.392156e-02 1.656855e-01 3 52695190 52695239 50 - 2.276 2.361 0.285
ENSG00000016864 E011 165.6419425 0.0026372964 4.867582e-04 2.109505e-03 3 52695240 52695273 34 - 2.159 2.287 0.428
ENSG00000016864 E012 158.3759433 0.0006383267 1.714192e-04 8.411868e-04 3 52695274 52695302 29 - 2.151 2.263 0.374
ENSG00000016864 E013 21.1578158 0.0008818448 1.778171e-01 2.983890e-01 3 52695303 52695420 118 - 1.302 1.401 0.345
ENSG00000016864 E014 281.7555908 0.0010897807 3.676536e-01 5.120077e-01 3 52695421 52695587 167 - 2.443 2.466 0.075
ENSG00000016864 E015 26.2047153 0.0007489329 8.782861e-01 9.258319e-01 3 52695588 52695927 340 - 1.443 1.433 -0.036
ENSG00000016864 E016 217.3845583 0.0021673743 9.629154e-01 9.805548e-01 3 52695928 52696040 113 - 2.342 2.341 -0.001
ENSG00000016864 E017 3.6827432 0.0183322329 7.145267e-01 8.106947e-01 3 52696197 52696233 37 - 0.634 0.686 0.220
ENSG00000016864 E018 88.0417729 0.0003371020 6.593383e-01 7.681737e-01 3 52696234 52696242 9 - 1.957 1.940 -0.060
ENSG00000016864 E019 161.7126722 0.0007044494 8.907494e-01 9.342225e-01 3 52696243 52696318 76 - 2.211 2.213 0.006
ENSG00000016864 E020 2.8303499 0.0322342913 2.456431e-02 6.098089e-02 3 52696319 52696541 223 - 0.733 0.332 -1.941
ENSG00000016864 E021 217.2416773 0.0029937304 1.401105e-01 2.481739e-01 3 52696542 52696659 118 - 2.365 2.307 -0.194
ENSG00000016864 E022 6.0517637 0.0026866091 8.852339e-01 9.306134e-01 3 52697505 52697720 216 - 0.849 0.830 -0.075
ENSG00000016864 E023 175.4719593 0.0043837537 1.849520e-02 4.822068e-02 3 52697721 52697781 61 - 2.292 2.187 -0.350
ENSG00000016864 E024 181.1762936 0.0015753660 5.075109e-03 1.613628e-02 3 52697782 52697859 78 - 2.301 2.207 -0.315
ENSG00000016864 E025 97.1833360 0.0028999798 1.789644e-01 2.998546e-01 3 52697860 52697862 3 - 2.020 1.956 -0.214
ENSG00000016864 E026 180.8202831 0.0027070692 4.954134e-02 1.083508e-01 3 52697863 52697934 72 - 2.293 2.217 -0.252
ENSG00000016864 E027 29.0353114 0.0098685542 1.441879e-14 4.643620e-13 3 52698414 52698678 265 - 1.060 1.705 2.245
ENSG00000016864 E028 132.7269075 0.0004030974 3.563924e-01 5.007726e-01 3 52700262 52700284 23 - 2.141 2.111 -0.100
ENSG00000016864 E029 173.5301701 0.0003486538 8.344022e-02 1.649484e-01 3 52700285 52700360 76 - 2.264 2.214 -0.166
ENSG00000016864 E030 124.6578776 0.0085524032 5.017728e-01 6.379137e-01 3 52700445 52700496 52 - 2.119 2.071 -0.160
ENSG00000016864 E031 5.7387351 0.0029268958 2.863505e-02 6.927991e-02 3 52700497 52700697 201 - 0.941 0.649 -1.161
ENSG00000016864 E032 3.2859509 0.0048179404 4.349320e-01 5.771765e-01 3 52701209 52701343 135 - 0.687 0.567 -0.523
ENSG00000016864 E033 0.9535628 0.0329458792 6.887354e-01 7.907902e-01 3 52702064 52702144 81 - 0.262 0.333 0.476
ENSG00000016864 E034 0.7437457 0.0153787590 3.082053e-01 4.504700e-01 3 52703087 52703255 169 - 0.323 0.141 -1.522
ENSG00000016864 E035 12.9075795 0.0014145101 5.316946e-01 6.641083e-01 3 52703504 52703698 195 - 1.170 1.111 -0.212
ENSG00000016864 E036 11.8065562 0.0019577822 1.842854e-01 3.066120e-01 3 52703799 52703928 130 - 1.170 1.040 -0.467
ENSG00000016864 E037 77.3337443 0.0089012052 4.651813e-02 1.029764e-01 3 52704724 52704812 89 - 1.950 1.820 -0.438
ENSG00000016864 E038 100.1540736 0.0018460589 5.612097e-05 3.122727e-04 3 52704813 52704948 136 - 2.075 1.905 -0.569
ENSG00000016864 E039 30.0106342 0.0043569974 2.263255e-02 5.698808e-02 3 52704949 52704952 4 - 1.560 1.394 -0.570
ENSG00000016864 E040 162.7687994 0.0071666467 3.803333e-02 8.741238e-02 3 52705447 52705698 252 - 2.262 2.149 -0.377
ENSG00000016864 E041 31.5176277 0.0093747621 1.650382e-02 4.385132e-02 3 52705699 52705702 4 - 1.589 1.395 -0.665
ENSG00000016864 E042 49.8641297 0.0019315080 6.756739e-06 4.670841e-05 3 52705703 52706032 330 - 1.805 1.555 -0.849