ENSG00000016391

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000315251 ENSG00000016391 HEK293_OSMI2_2hA HEK293_TMG_2hB CHDH protein_coding protein_coding 9.44333 11.09445 8.882798 0.3205418 0.2791878 -0.3204285 3.3355027 3.95507171 3.6586259 0.39156873 0.09942581 -0.1121072 0.3561625 0.355900000 0.4133333 0.05743333 0.56814041 0.01837926 FALSE  
MSTRG.23111.1 ENSG00000016391 HEK293_OSMI2_2hA HEK293_TMG_2hB CHDH protein_coding   9.44333 11.09445 8.882798 0.3205418 0.2791878 -0.3204285 3.8613404 4.08688120 2.9691549 0.51465384 0.12941067 -0.4596229 0.4078708 0.366433333 0.3358333 -0.03060000 0.83987998 0.01837926 TRUE  
MSTRG.23111.11 ENSG00000016391 HEK293_OSMI2_2hA HEK293_TMG_2hB CHDH protein_coding   9.44333 11.09445 8.882798 0.3205418 0.2791878 -0.3204285 0.3646252 0.03920597 0.7325977 0.03920597 0.35982591 3.9156757 0.0385125 0.003766667 0.0802000 0.07643333 0.01837926 0.01837926 FALSE  
MSTRG.23111.9 ENSG00000016391 HEK293_OSMI2_2hA HEK293_TMG_2hB CHDH protein_coding   9.44333 11.09445 8.882798 0.3205418 0.2791878 -0.3204285 1.2667125 2.31738293 0.9396232 0.55838135 0.24359172 -1.2932816 0.1325958 0.210533333 0.1043000 -0.10623333 0.30808416 0.01837926 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000016391 E001 0.8751994 0.1750778409 9.445180e-01 9.691961e-01 3 53803002 53803046 45 - 0.251 0.261 0.077
ENSG00000016391 E002 0.1515154 0.0424449367 3.471039e-01   3 53804039 53804077 39 - 0.143 0.000 -11.301
ENSG00000016391 E003 0.6568746 0.0191177703 5.113345e-01 6.463870e-01 3 53811940 53812264 325 - 0.143 0.258 1.052
ENSG00000016391 E004 0.4782907 0.0216103714 1.565252e-01 2.704773e-01 3 53812265 53812299 35 - 0.000 0.258 11.700
ENSG00000016391 E005 0.9263925 0.1454538827 2.870294e-01 4.274911e-01 3 53812300 53812334 35 - 0.144 0.365 1.750
ENSG00000016391 E006 1.2229789 0.3189185028 2.697027e-01 4.082390e-01 3 53812335 53812358 24 - 0.144 0.444 2.182
ENSG00000016391 E007 24.8550891 0.0053183225 4.592413e-02 1.018972e-01 3 53812359 53812805 447 - 1.296 1.455 0.550
ENSG00000016391 E008 14.3262296 0.0013573146 1.749985e-01 2.946693e-01 3 53812806 53812942 137 - 1.092 1.215 0.442
ENSG00000016391 E009 11.1596226 0.0019286537 8.398635e-01 9.000074e-01 3 53812943 53813055 113 - 1.063 1.082 0.070
ENSG00000016391 E010 11.9344256 0.0221681560 5.934057e-01 7.158116e-01 3 53813056 53813213 158 - 1.063 1.132 0.248
ENSG00000016391 E011 181.5260243 0.0006245103 1.262704e-01 2.289625e-01 3 53813214 53814162 949 - 2.217 2.259 0.140
ENSG00000016391 E012 45.4050503 0.0005216858 5.899110e-01 7.129034e-01 3 53814163 53814238 76 - 1.632 1.659 0.093
ENSG00000016391 E013 211.8060755 0.0036021079 1.233394e-02 3.433542e-02 3 53814239 53815726 1488 - 2.365 2.276 -0.298
ENSG00000016391 E014 51.3643917 0.0004337876 1.065864e-01 2.005092e-01 3 53815727 53815992 266 - 1.753 1.672 -0.273
ENSG00000016391 E015 47.3877013 0.0056489630 1.387735e-01 2.464054e-01 3 53815993 53816331 339 - 1.724 1.633 -0.311
ENSG00000016391 E016 741.5152796 0.0004138487 1.068345e-09 1.575669e-08 3 53816332 53818195 1864 - 2.802 2.881 0.261
ENSG00000016391 E017 106.3579171 0.0003115984 2.938642e-02 7.074721e-02 3 53818938 53819040 103 - 1.963 2.039 0.254
ENSG00000016391 E018 91.1267842 0.0002915557 4.527389e-01 5.935598e-01 3 53819532 53819674 143 - 1.930 1.957 0.090
ENSG00000016391 E019 111.9209245 0.0003290446 1.605515e-01 2.758646e-01 3 53820474 53820608 135 - 2.063 2.014 -0.164
ENSG00000016391 E020 74.4276970 0.0003315547 1.470449e-01 2.576628e-01 3 53821647 53821722 76 - 1.899 1.838 -0.205
ENSG00000016391 E021 55.2735258 0.0006794219 8.458778e-01 9.041663e-01 3 53821723 53821776 54 - 1.737 1.726 -0.037
ENSG00000016391 E022 91.1020025 0.0008703865 3.129077e-01 4.554260e-01 3 53822491 53822642 152 - 1.970 1.929 -0.138
ENSG00000016391 E023 1.0716524 0.0126177951 7.021833e-01 8.010461e-01 3 53823303 53823305 3 - 0.251 0.319 0.470
ENSG00000016391 E024 87.6641885 0.0076314569 3.547326e-01 4.990570e-01 3 53823306 53823543 238 - 1.959 1.907 -0.176
ENSG00000016391 E025 40.8112282 0.0033699307 1.306588e-01 2.350749e-01 3 53823544 53823630 87 - 1.659 1.566 -0.318
ENSG00000016391 E026 115.5997625 0.0007155381 3.919596e-02 8.954331e-02 3 53823631 53824067 437 - 2.092 2.019 -0.243
ENSG00000016391 E027 47.4911607 0.0005189332 2.557219e-04 1.199063e-03 3 53840929 53840999 71 - 1.779 1.593 -0.631
ENSG00000016391 E028 0.1817044 0.0392066960 6.712948e-01   3 53844582 53844617 36 - 0.000 0.104 9.929
ENSG00000016391 E029 0.0000000       3 53844713 53844769 57 -      
ENSG00000016391 E030 10.5366865 0.0038244428 8.700971e-05 4.610317e-04 3 53845822 53846082 261 - 1.260 0.859 -1.469
ENSG00000016391 E031 46.1128445 0.0092247204 3.205846e-04 1.463112e-03 3 53846083 53846441 359 - 1.796 1.547 -0.846