ENSG00000015475

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000342111 ENSG00000015475 HEK293_OSMI2_2hA HEK293_TMG_2hB BID protein_coding protein_coding 62.88984 113.334 39.2266 5.100738 1.212223 -1.530436 10.834761 23.087043 3.634771 3.1146850 1.1091126 -2.6638080 0.15082083 0.20210000 0.09226667 -0.10983333 5.218264e-02 2.971407e-11 FALSE TRUE
ENST00000399765 ENSG00000015475 HEK293_OSMI2_2hA HEK293_TMG_2hB BID protein_coding protein_coding 62.88984 113.334 39.2266 5.100738 1.212223 -1.530436 6.257098 8.864503 6.595667 0.1489591 0.0871427 -0.4259621 0.11054167 0.07853333 0.16856667 0.09003333 9.682246e-11 2.971407e-11 FALSE FALSE
ENST00000399767 ENSG00000015475 HEK293_OSMI2_2hA HEK293_TMG_2hB BID protein_coding protein_coding 62.88984 113.334 39.2266 5.100738 1.212223 -1.530436 29.990453 59.603885 15.604846 2.0691105 0.9294634 -1.9327301 0.45404167 0.52643333 0.39763333 -0.12880000 1.057951e-04 2.971407e-11 FALSE TRUE
ENST00000551952 ENSG00000015475 HEK293_OSMI2_2hA HEK293_TMG_2hB BID protein_coding protein_coding 62.88984 113.334 39.2266 5.100738 1.212223 -1.530436 11.533971 19.154703 7.759791 0.6249643 0.3950560 -1.3025039 0.19146250 0.16963333 0.19756667 0.02793333 3.286545e-01 2.971407e-11 FALSE TRUE
ENST00000622694 ENSG00000015475 HEK293_OSMI2_2hA HEK293_TMG_2hB BID protein_coding protein_coding 62.88984 113.334 39.2266 5.100738 1.212223 -1.530436 3.224738 1.183031 4.524912 0.3459035 0.1400536 1.9264427 0.07414167 0.01060000 0.11563333 0.10503333 2.971407e-11 2.971407e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000015475 E001 0.4427912 0.1790965650 1.684514e-01 2.861579e-01 22 17734138 17734141 4 - 0.329 0.074 -2.619
ENSG00000015475 E002 8.0216048 0.0068206481 1.825966e-02 4.769785e-02 22 17734142 17734174 33 - 1.092 0.794 -1.119
ENSG00000015475 E003 188.5241340 0.0045671024 2.576809e-51 1.689165e-48 22 17734175 17735221 1047 - 2.590 1.967 -2.083
ENSG00000015475 E004 61.8683526 0.0105023574 8.486499e-17 3.686636e-15 22 17735222 17735363 142 - 2.082 1.516 -1.915
ENSG00000015475 E005 382.0556262 0.0010549128 8.074455e-01 8.776659e-01 22 17735364 17735498 135 - 2.513 2.493 -0.066
ENSG00000015475 E006 429.5515463 0.0012099496 5.581380e-04 2.377995e-03 22 17735499 17735551 53 - 2.489 2.563 0.248
ENSG00000015475 E007 424.7725109 0.0015451056 1.065188e-01 2.004258e-01 22 17735552 17735591 40 - 2.520 2.550 0.098
ENSG00000015475 E008 459.3708144 0.0027992216 4.762713e-01 6.149265e-01 22 17738017 17738057 41 - 2.571 2.579 0.028
ENSG00000015475 E009 777.6266952 0.0001224741 5.280076e-06 3.737323e-05 22 17738058 17738229 172 - 2.762 2.816 0.178
ENSG00000015475 E010 622.4330565 0.0001686120 1.439516e-04 7.206787e-04 22 17739349 17739447 99 - 2.667 2.717 0.168
ENSG00000015475 E011 450.5430561 0.0002048362 3.699442e-01 5.143504e-01 22 17739448 17739488 41 - 2.563 2.567 0.012
ENSG00000015475 E012 4.5381581 0.0042899792 3.620271e-01 5.064348e-01 22 17739489 17740081 593 - 0.786 0.639 -0.605
ENSG00000015475 E013 2.3248203 0.0089657256 3.213173e-01 4.645429e-01 22 17740082 17740176 95 - 0.329 0.510 0.977
ENSG00000015475 E014 3.5353132 0.0045679585 1.591694e-02 4.254495e-02 22 17740177 17741194 1018 - 0.195 0.658 2.643
ENSG00000015475 E015 566.5806042 0.0019462738 9.592944e-02 1.842803e-01 22 17743803 17744013 211 - 2.643 2.673 0.100
ENSG00000015475 E016 277.4093559 0.0036690870 1.736540e-01 2.929365e-01 22 17750105 17750174 70 - 2.326 2.365 0.128
ENSG00000015475 E017 3.1094528 0.0140277084 2.820708e-01 4.220270e-01 22 17773610 17774016 407 - 0.697 0.510 -0.831
ENSG00000015475 E018 345.4507080 0.0051580633 6.842760e-01 7.872735e-01 22 17774381 17774495 115 - 2.450 2.453 0.011
ENSG00000015475 E019 2.1650893 0.0151538454 3.151545e-01 4.579193e-01 22 17774640 17774665 26 - 0.584 0.397 -0.926
ENSG00000015475 E020 0.2965864 0.2107000085 7.813827e-01   22 17774693 17774770 78 - 0.000 0.135 8.622