ENSG00000013374

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000413040 ENSG00000013374 HEK293_OSMI2_2hA HEK293_TMG_2hB NUB1 protein_coding protein_coding 59.41386 43.41457 78.50248 2.514386 4.537022 0.8544105 19.588002 16.70558 22.76956 0.9160415 0.4475970 0.4465466 0.3493042 0.3849667 0.2915333 -0.09343333 1.426234e-03 3.796255e-15 FALSE TRUE
ENST00000468404 ENSG00000013374 HEK293_OSMI2_2hA HEK293_TMG_2hB NUB1 protein_coding nonsense_mediated_decay 59.41386 43.41457 78.50248 2.514386 4.537022 0.8544105 21.557074 20.33288 27.59360 1.4469423 0.6501658 0.4403324 0.3699292 0.4675667 0.3530333 -0.11453333 1.901058e-04 3.796255e-15 TRUE TRUE
ENST00000483358 ENSG00000013374 HEK293_OSMI2_2hA HEK293_TMG_2hB NUB1 protein_coding protein_coding 59.41386 43.41457 78.50248 2.514386 4.537022 0.8544105 8.229373 0.00000 12.78829 0.0000000 4.0835555 10.3217351 0.1017250 0.0000000 0.1579667 0.15796667 3.796255e-15 3.796255e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000013374 E001 0.0000000       7 151341697 151341707 11 +      
ENSG00000013374 E002 0.1472490 0.0432608720 6.585384e-01   7 151341708 151341711 4 + 0.103 0.000 -9.273
ENSG00000013374 E003 0.4762024 0.2267365617 8.172594e-01 8.844447e-01 7 151341712 151341717 6 + 0.187 0.145 -0.441
ENSG00000013374 E004 5.9678939 0.0501348650 5.098588e-02 1.109224e-01 7 151341718 151341771 54 + 0.957 0.656 -1.188
ENSG00000013374 E005 16.8590941 0.0010238722 4.591429e-03 1.479508e-02 7 151341772 151341803 32 + 1.336 1.121 -0.758
ENSG00000013374 E006 21.7555961 0.0008626014 1.286657e-02 3.560313e-02 7 151341804 151341811 8 + 1.423 1.264 -0.556
ENSG00000013374 E007 117.0865030 0.0028536190 1.667006e-02 4.421428e-02 7 151341812 151341846 35 + 2.102 2.026 -0.253
ENSG00000013374 E008 17.0377705 0.0015111980 3.197234e-01 4.628106e-01 7 151341847 151341965 119 + 1.272 1.215 -0.203
ENSG00000013374 E009 1.4154708 0.0182929236 2.200096e-01 3.504212e-01 7 151345345 151345347 3 + 0.459 0.253 -1.253
ENSG00000013374 E010 309.5119956 0.0006535593 7.500641e-05 4.042600e-04 7 151345348 151345466 119 + 2.518 2.453 -0.218
ENSG00000013374 E011 428.0604180 0.0010913606 5.883862e-03 1.830646e-02 7 151349073 151349240 168 + 2.647 2.610 -0.123
ENSG00000013374 E012 0.0000000       7 151351120 151351327 208 +      
ENSG00000013374 E013 0.2998086 0.0286875825 8.191869e-01   7 151351328 151351423 96 + 0.103 0.144 0.552
ENSG00000013374 E014 294.9164799 0.0043103863 6.881592e-01 7.903398e-01 7 151351424 151351482 59 + 2.466 2.473 0.021
ENSG00000013374 E015 1.1844606 0.0102925134 3.162105e-01 4.589680e-01 7 151352093 151352193 101 + 0.417 0.252 -1.032
ENSG00000013374 E016 0.1482932 0.0410432671 3.604363e-01   7 151352252 151352366 115 + 0.000 0.144 10.102
ENSG00000013374 E017 272.3290447 0.0031381564 8.983576e-01 9.391825e-01 7 151352812 151352882 71 + 2.426 2.444 0.059
ENSG00000013374 E018 565.5621801 0.0001706485 2.515642e-04 1.181733e-03 7 151355768 151355950 183 + 2.765 2.736 -0.096
ENSG00000013374 E019 215.6224841 0.0002115253 3.738751e-02 8.620505e-02 7 151356128 151356130 3 + 2.347 2.324 -0.077
ENSG00000013374 E020 422.5394283 0.0011744092 1.451234e-01 2.550106e-01 7 151356131 151356222 92 + 2.630 2.624 -0.020
ENSG00000013374 E021 408.7571166 0.0007166866 1.159214e-01 2.141259e-01 7 151360141 151360247 107 + 2.615 2.610 -0.017
ENSG00000013374 E022 512.0273059 0.0030021861 1.338106e-03 5.103586e-03 7 151360248 151360914 667 + 2.650 2.774 0.413
ENSG00000013374 E023 73.6716052 0.0003654947 4.888319e-09 6.391589e-08 7 151361059 151361249 191 + 1.732 2.002 0.909
ENSG00000013374 E024 258.4187858 0.0003833913 3.083903e-02 7.360457e-02 7 151366939 151367125 187 + 2.423 2.400 -0.077
ENSG00000013374 E025 196.0354768 0.0008668764 2.254068e-01 3.569022e-01 7 151367861 151367968 108 + 2.296 2.288 -0.027
ENSG00000013374 E026 1.3296319 0.1264900995 8.441649e-01 9.029773e-01 7 151368733 151368734 2 + 0.370 0.342 -0.168
ENSG00000013374 E027 178.2880936 0.0001915838 3.989280e-03 1.311236e-02 7 151368735 151368829 95 + 2.275 2.226 -0.165
ENSG00000013374 E028 141.6793081 0.0012441115 7.558823e-02 1.524517e-01 7 151368830 151368887 58 + 2.171 2.138 -0.108
ENSG00000013374 E029 5.3928178 0.0030884463 1.849169e-03 6.755750e-03 7 151368888 151369193 306 + 0.944 0.527 -1.718
ENSG00000013374 E030 187.1903564 0.0023490161 8.131619e-01 8.815312e-01 7 151374097 151374201 105 + 2.267 2.285 0.063
ENSG00000013374 E031 16.7166921 0.0013495236 8.905301e-01 9.340822e-01 7 151374202 151374243 42 + 1.226 1.264 0.132
ENSG00000013374 E032 16.6257855 0.0010761649 4.599280e-01 5.999999e-01 7 151374244 151374624 381 + 1.260 1.225 -0.123
ENSG00000013374 E033 186.1710195 0.0011054796 5.903473e-01 7.132147e-01 7 151375848 151375943 96 + 2.268 2.279 0.040
ENSG00000013374 E034 282.8928070 0.0007479030 2.586693e-01 3.959801e-01 7 151376634 151376811 178 + 2.452 2.453 0.002
ENSG00000013374 E035 913.5800434 0.0039651824 9.621168e-08 9.797890e-07 7 151377047 151378429 1383 + 2.868 3.051 0.609
ENSG00000013374 E036 10.7335863 0.0156749406 1.022749e-01 1.939836e-01 7 151378430 151378449 20 + 0.970 1.188 0.793
ENSG00000013374 E037 5.7017240 0.0033676305 1.003043e-02 2.883745e-02 7 151378541 151378700 160 + 0.944 0.619 -1.303
ENSG00000013374 E038 1.9770317 0.2597611515 6.640965e-01 7.719751e-01 7 151379492 151379530 39 + 0.532 0.406 -0.634