ENSG00000013275

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000157812 ENSG00000013275 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC4 protein_coding protein_coding 126.9115 192.009 92.83302 10.49117 2.925028 -1.048384 112.15347 181.76378571 75.817915 10.31511471 1.907814 -1.261343 0.8669542 0.9464 0.81710000 -0.12930000 9.093507e-27 9.093507e-27 FALSE TRUE
ENST00000596386 ENSG00000013275 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC4 protein_coding retained_intron 126.9115 192.009 92.83302 10.49117 2.925028 -1.048384 5.82923 0.06058684 9.274942 0.06058684 1.542553 7.039350 0.0576750 0.0003 0.09933333 0.09903333 7.483193e-11 9.093507e-27 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000013275 E001 2.423066 8.056303e-03 9.635698e-01 9.810419e-01 19 39971040 39971122 83 + 0.490 0.481 -0.046
ENSG00000013275 E002 10.563279 1.744007e-03 1.034312e-01 1.957125e-01 19 39971123 39971164 42 + 1.132 0.959 -0.632
ENSG00000013275 E003 11.422936 1.547076e-03 1.407004e-01 2.489750e-01 19 39971165 39971169 5 + 1.148 0.996 -0.553
ENSG00000013275 E004 48.286651 4.826344e-04 3.067696e-10 4.984269e-09 19 39971170 39971179 10 + 1.852 1.524 -1.113
ENSG00000013275 E005 257.481297 2.114673e-03 6.021775e-07 5.244126e-06 19 39971180 39971191 12 + 2.466 2.302 -0.548
ENSG00000013275 E006 726.726736 2.651736e-03 1.193067e-02 3.336625e-02 19 39971192 39971238 47 + 2.853 2.777 -0.253
ENSG00000013275 E007 1129.991282 1.198767e-03 1.327864e-03 5.068501e-03 19 39972146 39972244 99 + 3.038 2.971 -0.222
ENSG00000013275 E008 648.632764 1.934097e-04 2.691042e-06 2.035847e-05 19 39972369 39972371 3 + 2.807 2.730 -0.258
ENSG00000013275 E009 1153.636935 1.253406e-03 4.112076e-02 9.310983e-02 19 39972372 39972461 90 + 3.030 2.986 -0.146
ENSG00000013275 E010 1112.956951 6.880707e-04 1.645275e-01 2.810571e-01 19 39972462 39972555 94 + 3.001 2.974 -0.090
ENSG00000013275 E011 1376.894639 1.124382e-03 6.271979e-06 4.367287e-05 19 39974294 39974440 147 + 3.137 3.050 -0.290
ENSG00000013275 E012 1241.246394 5.509422e-04 5.162528e-13 1.325597e-11 19 39974524 39974633 110 + 3.110 2.998 -0.372
ENSG00000013275 E013 1024.857556 2.635338e-04 5.380193e-01 6.694832e-01 19 39974735 39974828 94 + 2.954 2.943 -0.037
ENSG00000013275 E014 14.995943 2.564335e-03 3.033204e-01 4.452263e-01 19 39979444 39979816 373 + 1.222 1.125 -0.347
ENSG00000013275 E015 1484.681952 9.742394e-05 5.256801e-02 1.137323e-01 19 39979817 39979984 168 + 3.093 3.113 0.066
ENSG00000013275 E016 1.081130 3.608343e-01 5.716609e-01 6.978970e-01 19 39979985 39980061 77 + 0.412 0.250 -1.023
ENSG00000013275 E017 1249.477020 1.091534e-04 1.943107e-07 1.866334e-06 19 39980070 39980146 77 + 2.986 3.047 0.206
ENSG00000013275 E018 31.641258 1.708512e-03 2.470770e-08 2.828790e-07 19 39980147 39980285 139 + 1.691 1.328 -1.248
ENSG00000013275 E019 1926.451790 4.015134e-04 5.753364e-18 2.901138e-16 19 39980286 39980454 169 + 3.136 3.243 0.357
ENSG00000013275 E020 17.862214 2.308026e-03 1.947184e-06 1.518371e-05 19 39980455 39980661 207 + 1.481 1.091 -1.371
ENSG00000013275 E021 1288.270204 4.570657e-04 2.382953e-11 4.670201e-10 19 39980662 39980717 56 + 2.969 3.067 0.326
ENSG00000013275 E022 780.891618 8.798692e-04 1.258825e-08 1.523644e-07 19 39981192 39981193 2 + 2.739 2.854 0.382
ENSG00000013275 E023 1685.811059 1.924742e-03 3.338198e-03 1.125748e-02 19 39981194 39981764 571 + 3.113 3.176 0.209