ENSG00000008853

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000251822 ENSG00000008853 HEK293_OSMI2_2hA HEK293_TMG_2hB RHOBTB2 protein_coding protein_coding 4.395777 7.579518 3.519855 0.6529844 0.08062566 -1.104399 1.3920558 1.389192 1.590481 0.32664479 0.09453241 0.193912 0.33606250 0.1786000 0.4517000 0.2731000 6.145075e-04 5.850962e-18 FALSE TRUE
ENST00000522948 ENSG00000008853 HEK293_OSMI2_2hA HEK293_TMG_2hB RHOBTB2 protein_coding protein_coding 4.395777 7.579518 3.519855 0.6529844 0.08062566 -1.104399 2.4239670 4.350603 1.880764 0.17728727 0.13444161 -1.205558 0.57147917 0.5788333 0.5345333 -0.0443000 7.706527e-01 5.850962e-18 FALSE TRUE
ENST00000690180 ENSG00000008853 HEK293_OSMI2_2hA HEK293_TMG_2hB RHOBTB2 protein_coding retained_intron 4.395777 7.579518 3.519855 0.6529844 0.08062566 -1.104399 0.5248674 1.697987 0.000000 0.07952667 0.00000000 -7.416153 0.07864167 0.2259667 0.0000000 -0.2259667 5.850962e-18 5.850962e-18 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000008853 E001 0.0000000       8 22987417 22987481 65 +      
ENSG00000008853 E002 0.0000000       8 22987482 22987563 82 +      
ENSG00000008853 E003 0.0000000       8 22992031 22992067 37 +      
ENSG00000008853 E004 0.0000000       8 22992068 22992096 29 +      
ENSG00000008853 E005 0.0000000       8 22992097 22992180 84 +      
ENSG00000008853 E006 0.0000000       8 22994561 22994639 79 +      
ENSG00000008853 E007 0.0000000       8 22995841 22995882 42 +      
ENSG00000008853 E008 0.0000000       8 22996773 22997089 317 +      
ENSG00000008853 E009 0.0000000       8 22998923 22999161 239 +      
ENSG00000008853 E010 7.4313798 0.0029394585 1.949189e-04 9.422988e-04 8 22999548 22999928 381 + 1.131 0.709 -1.602
ENSG00000008853 E011 19.1009173 0.0011417309 2.608825e-04 1.220271e-03 8 22999929 23000105 177 + 1.430 1.155 -0.967
ENSG00000008853 E012 0.0000000       8 23004044 23004143 100 +      
ENSG00000008853 E013 38.0974401 0.0005487935 3.860204e-03 1.275025e-02 8 23004425 23004626 202 + 1.649 1.494 -0.528
ENSG00000008853 E014 0.5149111 0.0204486075 1.000000e+00 1.000000e+00 8 23004627 23004844 218 + 0.165 0.164 -0.018
ENSG00000008853 E015 36.0838756 0.0006022309 3.151903e-02 7.491702e-02 8 23005372 23005475 104 + 1.601 1.484 -0.399
ENSG00000008853 E016 44.6561046 0.0005232753 1.913636e-02 4.963684e-02 8 23005960 23006145 186 + 1.692 1.578 -0.387
ENSG00000008853 E017 146.1050210 0.0002506044 3.520813e-03 1.178720e-02 8 23006728 23007746 1019 + 2.171 2.099 -0.243
ENSG00000008853 E018 0.9827075 0.3048393493 4.302977e-01 5.729328e-01 8 23007839 23007992 154 + 0.378 0.228 -1.002
ENSG00000008853 E019 49.0462930 0.0005262814 3.898946e-01 5.339374e-01 8 23007993 23008111 119 + 1.611 1.670 0.200
ENSG00000008853 E020 0.1515154 0.0429798449 2.355798e-01   8 23010368 23010537 170 + 0.165 0.000 -9.612
ENSG00000008853 E021 57.1288623 0.0004531091 6.902529e-01 7.919905e-01 8 23010538 23010688 151 + 1.723 1.716 -0.024
ENSG00000008853 E022 49.0591081 0.0009944902 6.585588e-01 7.676575e-01 8 23014690 23014778 89 + 1.658 1.646 -0.039
ENSG00000008853 E023 53.3847925 0.0005242673 2.202258e-02 5.572623e-02 8 23015638 23015743 106 + 1.756 1.655 -0.344
ENSG00000008853 E024 542.4923275 0.0008422931 1.513197e-11 3.059562e-10 8 23017252 23020509 3258 + 2.610 2.719 0.363