ENSG00000006327

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000326577 ENSG00000006327 HEK293_OSMI2_2hA HEK293_TMG_2hB TNFRSF12A protein_coding protein_coding 17.32272 26.17233 11.4703 2.283904 0.4688186 -1.189434 14.3287042 22.033827 9.3628450 1.62574923 0.50321127 -1.2338158 0.82329583 0.84446667 0.81570000 -0.0287666667 7.269681e-01 4.959493e-05 FALSE  
ENST00000341627 ENSG00000006327 HEK293_OSMI2_2hA HEK293_TMG_2hB TNFRSF12A protein_coding protein_coding 17.32272 26.17233 11.4703 2.283904 0.4688186 -1.189434 1.8667293 2.799237 1.1921203 0.94395904 0.26097771 -1.2245972 0.10227917 0.10470000 0.10386667 -0.0008333333 9.827827e-01 4.959493e-05 FALSE  
ENST00000571351 ENSG00000006327 HEK293_OSMI2_2hA HEK293_TMG_2hB TNFRSF12A protein_coding retained_intron 17.32272 26.17233 11.4703 2.283904 0.4688186 -1.189434 0.7649471 0.519408 0.7968453 0.07829928 0.06442212 0.6079121 0.05179167 0.01993333 0.07016667 0.0502333333 4.959493e-05 4.959493e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000006327 E001 0.0000000       16 3018445 3018536 92 +      
ENSG00000006327 E002 0.8147328 0.1377141331 6.696044e-01 0.7763834401 16 3020359 3020367 9 + 0.301 0.213 -0.655
ENSG00000006327 E003 2.5442048 0.0912498698 5.512419e-01 0.6807410889 16 3020368 3020374 7 + 0.601 0.490 -0.519
ENSG00000006327 E004 4.9370951 0.0713135211 5.108044e-01 0.6459668715 16 3020375 3020378 4 + 0.812 0.699 -0.455
ENSG00000006327 E005 22.0943327 0.0222828930 8.063844e-01 0.8769286764 16 3020379 3020389 11 + 1.321 1.299 -0.079
ENSG00000006327 E006 70.5600691 0.0056853778 9.958232e-01 1.0000000000 16 3020390 3020491 102 + 1.788 1.792 0.014
ENSG00000006327 E007 10.0554323 0.0200590477 9.610981e-04 0.0038253911 16 3020492 3020759 268 + 1.242 0.854 -1.420
ENSG00000006327 E008 11.0538201 0.0052588955 5.514744e-05 0.0003074246 16 3020760 3021159 400 + 1.289 0.880 -1.486
ENSG00000006327 E009 2.1045831 0.1392369604 5.082661e-01 0.6437237635 16 3021160 3021188 29 + 0.543 0.401 -0.717
ENSG00000006327 E010 2.7593814 0.0058320998 5.192443e-01 0.6533645872 16 3021189 3021214 26 + 0.601 0.495 -0.493
ENSG00000006327 E011 95.2453413 0.0003032416 9.601335e-01 0.9789089159 16 3021215 3021319 105 + 1.920 1.923 0.007
ENSG00000006327 E012 152.5593879 0.0003630463 8.383645e-01 0.8989604751 16 3021555 3021689 135 + 2.118 2.124 0.022
ENSG00000006327 E013 342.9859393 0.0011567000 3.288279e-02 0.0775678251 16 3021771 3022383 613 + 2.446 2.487 0.137