ENSG00000005486

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000006777 ENSG00000005486 HEK293_OSMI2_2hA HEK293_TMG_2hB RHBDD2 protein_coding protein_coding 65.53197 109.1294 45.34513 2.002244 0.6831373 -1.266835 51.150281 74.709382 40.418453 6.157949 0.04590732 -0.8861115 0.8133625 0.68296667 0.8917667 0.20880000 8.023233e-05 6.192206e-20 FALSE TRUE
ENST00000428119 ENSG00000005486 HEK293_OSMI2_2hA HEK293_TMG_2hB RHBDD2 protein_coding protein_coding 65.53197 109.1294 45.34513 2.002244 0.6831373 -1.266835 7.235806 22.538222 0.000000 5.443336 0.00000000 -11.1387980 0.0750250 0.20850000 0.0000000 -0.20850000 6.192206e-20 6.192206e-20 FALSE TRUE
ENST00000454791 ENSG00000005486 HEK293_OSMI2_2hA HEK293_TMG_2hB RHBDD2 protein_coding nonsense_mediated_decay 65.53197 109.1294 45.34513 2.002244 0.6831373 -1.266835 4.200618 5.391414 3.271529 2.752786 1.64830404 -0.7189691 0.0670000 0.04983333 0.0712000 0.02136667 9.769478e-01 6.192206e-20 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000005486 E001 0.0000000       7 75842602 75842749 148 +      
ENSG00000005486 E002 0.0000000       7 75878091 75878186 96 +      
ENSG00000005486 E003 0.5483223 2.112947e-02 8.702640e-01 9.205462e-01 7 75878999 75879003 5 + 0.173 0.157 -0.170
ENSG00000005486 E004 16.9939671 6.210945e-03 7.873062e-01 8.637776e-01 7 75879004 75879033 30 + 1.152 1.211 0.209
ENSG00000005486 E005 232.6709275 2.952613e-03 7.544828e-03 2.263054e-02 7 75879034 75879139 106 + 2.355 2.296 -0.198
ENSG00000005486 E006 196.4572340 2.729216e-03 4.984002e-04 2.154045e-03 7 75879140 75879171 32 + 2.305 2.214 -0.305
ENSG00000005486 E007 372.7155608 2.175130e-03 8.793514e-05 4.653105e-04 7 75879172 75879260 89 + 2.572 2.496 -0.254
ENSG00000005486 E008 1.3599215 2.311168e-02 2.292183e-01 3.615051e-01 7 75880246 75880305 60 + 0.470 0.272 -1.169
ENSG00000005486 E009 52.4183131 4.315980e-03 5.498564e-01 6.795528e-01 7 75881365 75881486 122 + 1.671 1.668 -0.011
ENSG00000005486 E010 754.9268053 9.037496e-04 9.021385e-07 7.562611e-06 7 75881829 75882043 215 + 2.862 2.808 -0.177
ENSG00000005486 E011 715.6412917 1.773346e-04 1.708676e-03 6.308903e-03 7 75882044 75882236 193 + 2.808 2.797 -0.039
ENSG00000005486 E012 10.3573415 6.363103e-03 2.380669e-01 3.718969e-01 7 75883490 75883697 208 + 0.866 1.041 0.654
ENSG00000005486 E013 756.1664372 9.559944e-05 9.252295e-01 9.569385e-01 7 75883698 75883848 151 + 2.798 2.833 0.118
ENSG00000005486 E014 2152.6177409 2.785297e-03 5.182054e-08 5.570701e-07 7 75887992 75888926 935 + 3.180 3.312 0.441