ENSG00000005469

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000331536 ENSG00000005469 HEK293_OSMI2_2hA HEK293_TMG_2hB CROT protein_coding protein_coding 4.940026 2.183883 6.908986 0.08130641 0.11861 1.657074 3.4520282 1.2790530 5.1516493 0.04520538 0.23463719 2.0015206 0.7004167 0.5870667 0.74493333 0.15786667 3.316475e-02 5.256476e-05 FALSE TRUE
ENST00000412227 ENSG00000005469 HEK293_OSMI2_2hA HEK293_TMG_2hB CROT protein_coding protein_coding 4.940026 2.183883 6.908986 0.08130641 0.11861 1.657074 0.5541255 0.6300168 0.4771160 0.13712154 0.04254612 -0.3938444 0.1383542 0.2846333 0.06930000 -0.21533333 5.256476e-05 5.256476e-05   FALSE
ENST00000479014 ENSG00000005469 HEK293_OSMI2_2hA HEK293_TMG_2hB CROT protein_coding retained_intron 4.940026 2.183883 6.908986 0.08130641 0.11861 1.657074 0.4337384 0.1798536 0.4706691 0.11430387 0.13390402 1.3401568 0.0721750 0.0851000 0.06813333 -0.01696667 1.000000e+00 5.256476e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000005469 E001 1.1115096 0.0482299384 6.109958e-01 7.302185e-01 7 87345664 87345680 17 + 0.310 0.207 -0.767
ENSG00000005469 E002 2.9248340 0.0054991375 4.737491e-01 6.126582e-01 7 87345681 87345692 12 + 0.577 0.452 -0.601
ENSG00000005469 E003 11.5857549 0.0058841190 1.590091e-01 2.737979e-01 7 87345693 87345767 75 + 1.001 1.159 0.572
ENSG00000005469 E004 0.0000000       7 87346335 87346339 5 +      
ENSG00000005469 E005 16.8101029 0.0011629409 1.147132e-01 2.124140e-01 7 87346340 87346430 91 + 1.152 1.299 0.520
ENSG00000005469 E006 26.2615556 0.0047233439 8.956556e-02 1.744594e-01 7 87349048 87349183 136 + 1.335 1.473 0.478
ENSG00000005469 E007 0.6674587 0.0294121480 9.073903e-01 9.451937e-01 7 87357507 87357590 84 + 0.182 0.207 0.231
ENSG00000005469 E008 28.1256364 0.0007531649 1.635554e-01 2.797598e-01 7 87359206 87359330 125 + 1.375 1.481 0.365
ENSG00000005469 E009 12.6358359 0.0262638898 1.670976e-08 1.976162e-07 7 87359331 87360116 786 + 0.793 1.469 2.448
ENSG00000005469 E010 16.1407766 0.0059012429 4.337097e-01 5.761154e-01 7 87361390 87361442 53 + 1.197 1.121 -0.273
ENSG00000005469 E011 21.0037947 0.0009968277 1.550647e-01 2.684922e-01 7 87361443 87361571 129 + 1.314 1.194 -0.421
ENSG00000005469 E012 15.9497889 0.0123485415 4.468580e-03 1.445331e-02 7 87361728 87361852 125 + 1.238 0.888 -1.276
ENSG00000005469 E013 18.2063810 0.0012258341 1.448543e-02 3.932731e-02 7 87369376 87369484 109 + 1.272 1.032 -0.858
ENSG00000005469 E014 0.4460135 0.0390041656 5.113743e-01 6.464243e-01 7 87369485 87369696 212 + 0.182 0.001 -8.008
ENSG00000005469 E015 1.9367272 0.0299157625 1.654663e-01 2.823121e-01 7 87375235 87375631 397 + 0.489 0.207 -1.775
ENSG00000005469 E016 18.6216232 0.0010097885 6.399833e-02 1.333512e-01 7 87375632 87375725 94 + 1.272 1.100 -0.610
ENSG00000005469 E017 11.9752385 0.0026009020 5.950863e-01 7.172315e-01 7 87375828 87375872 45 + 1.037 1.100 0.230
ENSG00000005469 E018 15.7777995 0.0243193051 3.556768e-01 5.000409e-01 7 87375873 87375953 81 + 1.140 1.243 0.365
ENSG00000005469 E019 24.3474653 0.0007764294 8.452388e-01 9.037273e-01 7 87377349 87377450 102 + 1.339 1.361 0.077
ENSG00000005469 E020 27.9671711 0.0007211682 2.229894e-01 3.539414e-01 7 87381910 87381993 84 + 1.423 1.337 -0.298
ENSG00000005469 E021 32.4457352 0.0006004002 6.235232e-01 7.400966e-01 7 87382074 87382181 108 + 1.472 1.445 -0.093
ENSG00000005469 E022 2.1661240 0.0094239119 5.964116e-01 7.183626e-01 7 87382182 87382329 148 + 0.437 0.537 0.492
ENSG00000005469 E023 30.5257565 0.0042601409 2.048962e-02 5.252350e-02 7 87382413 87382543 131 + 1.480 1.299 -0.624
ENSG00000005469 E024 30.1167836 0.0161642751 7.521570e-01 8.384459e-01 7 87391589 87391712 124 + 1.441 1.424 -0.056
ENSG00000005469 E025 24.1369881 0.0009463413 1.018198e-01 1.932754e-01 7 87392566 87392644 79 + 1.372 1.242 -0.454
ENSG00000005469 E026 24.1761372 0.0008654178 5.965033e-02 1.260250e-01 7 87392730 87392823 94 + 1.379 1.227 -0.530
ENSG00000005469 E027 24.5268118 0.0046981064 1.284448e-02 3.555084e-02 7 87392948 87393067 120 + 1.397 1.177 -0.770
ENSG00000005469 E028 1.6189451 0.0089023180 2.175998e-01 3.475004e-01 7 87398317 87398523 207 + 0.310 0.537 1.229
ENSG00000005469 E029 104.3652554 0.0012090666 3.026335e-03 1.034388e-02 7 87398524 87399794 1271 + 1.932 2.049 0.393