ENSG00000005448

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000348227 ENSG00000005448 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR54 protein_coding protein_coding 88.10297 158.8215 54.09613 6.417225 0.1683975 -1.553633 73.90071 139.9412 44.36408 4.818082 0.1872386 -1.657135 0.8187875 0.8816 0.8201333 -0.06146667 0.0001185187 4.925835e-29 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000005448 E001 0.7019140 0.0163965302 2.447170e-01 3.797180e-01 2 74421678 74421717 40 + 0.340 0.133 -1.731
ENSG00000005448 E002 0.5546650 0.0204896324 6.704684e-01 7.768875e-01 2 74421718 74421718 1 + 0.202 0.133 -0.732
ENSG00000005448 E003 0.9922594 0.0121989077 2.469414e-02 6.125391e-02 2 74421719 74421720 2 + 0.528 0.133 -2.733
ENSG00000005448 E004 1.3222570 0.0102902184 1.009628e-01 1.919403e-01 2 74421721 74421721 1 + 0.528 0.234 -1.734
ENSG00000005448 E005 2.4637520 0.0061558674 3.676352e-01 5.119993e-01 2 74421722 74421725 4 + 0.599 0.444 -0.735
ENSG00000005448 E006 4.0127502 0.0041242867 7.329551e-01 8.243051e-01 2 74421726 74421728 3 + 0.659 0.606 -0.231
ENSG00000005448 E007 4.6039338 0.0036069107 9.940523e-01 1.000000e+00 2 74421729 74421729 1 + 0.659 0.660 0.004
ENSG00000005448 E008 4.9736722 0.0035580711 8.426220e-01 9.019381e-01 2 74421730 74421730 1 + 0.659 0.693 0.141
ENSG00000005448 E009 11.0279195 0.0015629442 6.528627e-01 7.634958e-01 2 74421731 74421737 7 + 0.940 0.997 0.210
ENSG00000005448 E010 33.1659203 0.0013025510 1.513862e-01 2.635561e-01 2 74421738 74421743 6 + 1.338 1.452 0.396
ENSG00000005448 E011 91.1410566 0.0073159919 4.895052e-03 1.563859e-02 2 74421744 74421758 15 + 1.990 1.828 -0.545
ENSG00000005448 E012 185.0121189 0.0096254530 1.838710e-01 3.060806e-01 2 74421759 74421763 5 + 2.223 2.155 -0.227
ENSG00000005448 E013 201.2434612 0.0078588379 2.068381e-01 3.347646e-01 2 74421764 74421765 2 + 2.251 2.193 -0.194
ENSG00000005448 E014 379.1507504 0.0050979285 4.528143e-01 5.936260e-01 2 74421766 74421816 51 + 2.498 2.476 -0.075
ENSG00000005448 E015 44.8960098 0.0076382139 5.598693e-01 6.879512e-01 2 74421817 74422119 303 + 1.591 1.551 -0.135
ENSG00000005448 E016 34.4604957 0.0011185894 5.779974e-01 7.031573e-01 2 74422120 74422152 33 + 1.478 1.441 -0.128
ENSG00000005448 E017 385.2674489 0.0035271780 6.450748e-01 7.572700e-01 2 74422153 74422174 22 + 2.494 2.485 -0.031
ENSG00000005448 E018 819.5714081 0.0010449427 1.229420e-01 2.241523e-01 2 74422175 74422375 201 + 2.835 2.808 -0.088
ENSG00000005448 E019 17.8978641 0.0034509032 2.129620e-10 3.549310e-09 2 74422376 74422869 494 + 1.549 1.019 -1.866
ENSG00000005448 E020 569.4133174 0.0001151441 1.749718e-03 6.439231e-03 2 74422870 74422932 63 + 2.698 2.645 -0.175
ENSG00000005448 E021 9.2709873 0.0031178723 1.163563e-05 7.616651e-05 2 74422933 74423111 179 + 1.246 0.765 -1.785
ENSG00000005448 E022 8.7141719 0.0019580600 1.341621e-05 8.651578e-05 2 74423191 74423318 128 + 1.231 0.751 -1.788
ENSG00000005448 E023 624.3227634 0.0005148332 1.615110e-02 4.305966e-02 2 74423319 74423385 67 + 2.731 2.688 -0.143
ENSG00000005448 E024 10.0562952 0.0096704264 2.628764e-02 6.452598e-02 2 74423386 74423477 92 + 1.148 0.891 -0.948
ENSG00000005448 E025 395.5562027 0.0001289049 3.025777e-03 1.034234e-02 2 74423478 74423482 5 + 2.546 2.486 -0.200
ENSG00000005448 E026 571.6645763 0.0001149430 6.398682e-02 1.333302e-01 2 74423483 74423527 45 + 2.682 2.652 -0.100
ENSG00000005448 E027 431.6456560 0.0002435589 2.439488e-02 6.063260e-02 2 74423528 74423531 4 + 2.570 2.525 -0.148
ENSG00000005448 E028 21.1108054 0.0010934755 2.674961e-07 2.500929e-06 2 74423532 74423854 323 + 1.535 1.136 -1.392
ENSG00000005448 E029 999.9292875 0.0002456379 2.145052e-01 3.438711e-01 2 74423855 74423982 128 + 2.913 2.898 -0.049
ENSG00000005448 E030 3.6911717 0.0043139187 5.597108e-01 6.877864e-01 2 74424873 74424874 2 + 0.659 0.566 -0.411
ENSG00000005448 E031 815.5757747 0.0001676243 1.487455e-02 4.020745e-02 2 74424875 74424975 101 + 2.779 2.821 0.139
ENSG00000005448 E032 6.1729092 0.0031835386 4.608667e-02 1.021956e-01 2 74424976 74425074 99 + 0.969 0.708 -1.018
ENSG00000005448 E033 546.1859707 0.0001209278 1.029977e-03 4.062080e-03 2 74425075 74425096 22 + 2.583 2.649 0.219
ENSG00000005448 E034 1038.5347583 0.0005691880 2.890816e-06 2.170742e-05 2 74425097 74425237 141 + 2.850 2.934 0.280
ENSG00000005448 E035 724.0728719 0.0019016507 4.658078e-05 2.644230e-04 2 74425417 74425491 75 + 2.671 2.783 0.370
ENSG00000005448 E036 517.1212002 0.0019637989 5.889708e-03 1.832163e-02 2 74425570 74425639 70 + 2.548 2.632 0.279
ENSG00000005448 E037 426.5299041 0.0014644175 4.512988e-03 1.457977e-02 2 74425640 74425755 116 + 2.464 2.549 0.282