ENSG00000156976

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323963 ENSG00000156976 HEK293_DMSO_6hB HEK293_TMG_6hB EIF4A2 protein_coding protein_coding 99.25201 172.7007 180.6094 7.209073 19.59903 0.06459548 24.766467 58.6929200 30.45042 0.9396052 1.7639018 -0.9464961991 0.25995417 0.341300000 0.17413333 -0.1671666667 0.04662932 0.04662932 FALSE TRUE
ENST00000363450 ENSG00000156976 HEK293_DMSO_6hB HEK293_TMG_6hB EIF4A2 protein_coding snoRNA 99.25201 172.7007 180.6094 7.209073 19.59903 0.06459548 2.946017 3.0647137 17.24859 3.0647137 17.2485854 2.4887902466 0.02050417 0.018866667 0.07863333 0.0597666667 1.00000000 0.04662932   FALSE
ENST00000485101 ENSG00000156976 HEK293_DMSO_6hB HEK293_TMG_6hB EIF4A2 protein_coding retained_intron 99.25201 172.7007 180.6094 7.209073 19.59903 0.06459548 6.777684 11.4995914 12.68792 1.4197858 0.1626560 0.1417556678 0.06282500 0.066166667 0.07186667 0.0057000000 0.96729223 0.04662932   FALSE
ENST00000497177 ENSG00000156976 HEK293_DMSO_6hB HEK293_TMG_6hB EIF4A2 protein_coding retained_intron 99.25201 172.7007 180.6094 7.209073 19.59903 0.06459548 12.382742 20.7510036 20.75940 2.8308284 0.9777344 0.0005834895 0.12561667 0.119200000 0.11823333 -0.0009666667 1.00000000 0.04662932   FALSE
MSTRG.24239.18 ENSG00000156976 HEK293_DMSO_6hB HEK293_TMG_6hB EIF4A2 protein_coding   99.25201 172.7007 180.6094 7.209073 19.59903 0.06459548 7.942358 11.5231322 15.78612 0.9759586 1.4608386 0.4537854201 0.07709167 0.066533333 0.09093333 0.0244000000 0.95053252 0.04662932 TRUE TRUE
MSTRG.24239.4 ENSG00000156976 HEK293_DMSO_6hB HEK293_TMG_6hB EIF4A2 protein_coding   99.25201 172.7007 180.6094 7.209073 19.59903 0.06459548 2.354658 0.8320124 10.31973 0.8320124 2.0464441 3.6168171158 0.02317917 0.004466667 0.05770000 0.0532333333 0.34486642 0.04662932 TRUE TRUE
MSTRG.24239.7 ENSG00000156976 HEK293_DMSO_6hB HEK293_TMG_6hB EIF4A2 protein_coding   99.25201 172.7007 180.6094 7.209073 19.59903 0.06459548 13.411300 23.8525837 18.72783 1.7946930 0.9698200 -0.3487964309 0.13066250 0.139166667 0.10703333 -0.0321333333 0.95053252 0.04662932 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_DMSO_6hB log2fold_HEK293_DMSO_6hB_HEK293_TMG_6hB
ENSG00000156976 E001 0.1538588 0.0452247717 5.642799e-01   3 186783205 186783304 100 + 0.000 0.117 10.212
ENSG00000156976 E002 0.1538588 0.0452247717 5.642799e-01   3 186783305 186783350 46 + 0.000 0.117 10.257
ENSG00000156976 E003 0.6347634 0.0303724172 3.946968e-01   3 186783351 186783447 97 + 0.131 0.285 1.396
ENSG00000156976 E004 1.2724613 0.0148440099 8.290214e-01 0.97900257 3 186783448 186783571 124 + 0.381 0.349 -0.188
ENSG00000156976 E005 0.7915567 0.0165868579 5.376479e-01   3 186783572 186783573 2 + 0.313 0.209 -0.774
ENSG00000156976 E006 0.7915567 0.0165868579 5.376479e-01   3 186783574 186783574 1 + 0.313 0.209 -0.774
ENSG00000156976 E007 0.9416775 0.0279320948 3.252154e-01   3 186783575 186783576 2 + 0.381 0.209 -1.187
ENSG00000156976 E008 0.7915567 0.0165868579 5.376479e-01   3 186783577 186783577 1 + 0.313 0.209 -0.774
ENSG00000156976 E009 0.7915567 0.0165868579 5.376479e-01   3 186783578 186783585 8 + 0.313 0.209 -0.774
ENSG00000156976 E010 131.1919795 1.2530545161 4.590330e-01 0.94041302 3 186783586 186783592 7 + 2.003 2.206 0.680
ENSG00000156976 E011 186.9815107 1.3751122103 4.717432e-01 0.94041302 3 186783593 186783596 4 + 2.152 2.361 0.701
ENSG00000156976 E012 287.7801781 1.5074626518 4.645074e-01 0.94041302 3 186783597 186783639 43 + 2.321 2.557 0.788
ENSG00000156976 E013 21.6728550 0.0013768450 5.229624e-01 0.94041302 3 186784203 186784428 226 + 1.372 1.336 -0.125
ENSG00000156976 E014 81.5011106 0.1853266186 2.121214e-01 0.94041302 3 186784429 186784431 3 + 1.805 1.998 0.648
ENSG00000156976 E015 394.1378853 0.0964900198 1.392508e-01 0.94041302 3 186784432 186784477 46 + 2.491 2.675 0.613
ENSG00000156976 E016 68.3769719 0.0277584463 3.902111e-01 0.94041302 3 186784478 186784563 86 + 1.801 1.876 0.254
ENSG00000156976 E017 658.0582184 0.0102008167 5.318266e-02 0.94041302 3 186784564 186784696 133 + 2.762 2.865 0.342
ENSG00000156976 E018 95.4800987 0.0005812125 9.468679e-04 0.44241375 3 186784697 186784795 99 + 2.038 1.926 -0.376
ENSG00000156976 E019 123.1591307 0.0104587661 3.433645e-02 0.92466706 3 186784796 186784961 166 + 2.144 2.039 -0.351
ENSG00000156976 E020 479.7942155 0.0022053631 5.171517e-03 0.67546036 3 186784962 186785060 99 + 2.633 2.722 0.298
ENSG00000156976 E021 432.7720370 0.0029930277 1.987705e-02 0.85996662 3 186785061 186785101 41 + 2.591 2.675 0.283
ENSG00000156976 E022 239.2016118 0.0102809989 8.219516e-03 0.75617574 3 186785102 186785882 781 + 2.439 2.317 -0.407
ENSG00000156976 E023 699.0035135 0.0001042734 2.870431e-02 0.89965206 3 186785883 186785961 79 + 2.822 2.864 0.142
ENSG00000156976 E024 546.6657666 0.0001118012 1.465312e-01 0.94041302 3 186785962 186785994 33 + 2.719 2.754 0.116
ENSG00000156976 E025 684.3690202 0.0005337398 3.925893e-01 0.94041302 3 186785995 186786051 57 + 2.821 2.848 0.091
ENSG00000156976 E026 9.0431410 0.0405585277 3.977527e-01 0.94041302 3 186786052 186786075 24 + 0.923 1.061 0.511
ENSG00000156976 E027 23.8522081 0.0014358689 1.570507e-01 0.94041302 3 186786076 186786163 88 + 1.438 1.348 -0.311
ENSG00000156976 E028 861.2004840 0.0027639573 8.531358e-01 0.98272447 3 186786164 186786273 110 + 2.930 2.939 0.028
ENSG00000156976 E029 39.3609391 0.0013399078 8.398511e-02 0.94041302 3 186786274 186786322 49 + 1.650 1.562 -0.299
ENSG00000156976 E030 72.7658348 0.0015954635 3.805823e-04 0.31623557 3 186786323 186786490 168 + 1.938 1.789 -0.501
ENSG00000156976 E031 30.1564299 0.0011161116 1.218800e-01 0.94041302 3 186786491 186786501 11 + 1.537 1.449 -0.301
ENSG00000156976 E032 966.5030999 0.0011492740 8.882792e-01 0.98803451 3 186786502 186786639 138 + 2.979 2.990 0.035
ENSG00000156976 E033 432.3581948 0.0006990735 4.646800e-01 0.94041302 3 186786640 186786645 6 + 2.620 2.649 0.094
ENSG00000156976 E034 34.1345626 0.0010015633 3.963558e-01 0.94041302 3 186786646 186786671 26 + 1.563 1.524 -0.135
ENSG00000156976 E035 27.4637676 0.0014386728 2.205027e-01 0.94041302 3 186786672 186786674 3 + 1.486 1.415 -0.244
ENSG00000156976 E036 149.4020449 0.0068532445 1.244136e-01 0.94041302 3 186786675 186787126 452 + 2.207 2.144 -0.211
ENSG00000156976 E037 930.1046152 0.0013549680 5.246528e-01 0.94041302 3 186787127 186787264 138 + 2.955 2.980 0.085
ENSG00000156976 E038 40.6609194 0.0009594005 1.616517e-01 0.94041302 3 186787265 186787299 35 + 1.650 1.584 -0.226
ENSG00000156976 E039 30.1277131 0.0025683605 8.956474e-03 0.76729502 3 186787300 186787313 14 + 1.568 1.404 -0.560
ENSG00000156976 E040 71.3480731 0.0339661798 8.393662e-02 0.94041302 3 186787314 186787406 93 + 1.939 1.767 -0.583
ENSG00000156976 E041 81.0802243 0.0369689966 3.136471e-01 0.94041302 3 186787407 186787478 72 + 1.964 1.859 -0.352
ENSG00000156976 E042 54.9274321 0.0006947146 5.021574e-01 0.94041302 3 186787479 186787494 16 + 1.758 1.738 -0.069
ENSG00000156976 E043 718.6376201 0.0007616728 7.755915e-01 0.97175201 3 186787495 186787584 90 + 2.847 2.865 0.058
ENSG00000156976 E044 40.3256419 0.0009401784 3.015835e-04 0.29069620 3 186787585 186787611 27 + 1.707 1.516 -0.654
ENSG00000156976 E045 104.8894795 0.0004040657 5.374702e-06 0.03091134 3 186787612 186787775 164 + 2.095 1.946 -0.501
ENSG00000156976 E046 55.7703812 0.0009475579 2.540063e-01 0.94041302 3 186787776 186787802 27 + 1.777 1.733 -0.147
ENSG00000156976 E047 751.9307488 0.0003442451 5.648657e-01 0.94134549 3 186787803 186787882 80 + 2.865 2.886 0.071
ENSG00000156976 E048 475.3742258 0.0069884697 3.958897e-02 0.94020585 3 186787883 186788309 427 + 2.712 2.643 -0.232
ENSG00000156976 E049 565.1009428 0.0010875917 4.126110e-06 0.02542538 3 186788310 186788416 107 + 2.794 2.709 -0.283
ENSG00000156976 E050 255.8727495 0.0016746295 6.021409e-03 0.70526129 3 186788417 186788420 4 + 2.442 2.375 -0.225
ENSG00000156976 E051 926.3027353 0.0038942730 4.803478e-03 0.65932453 3 186788421 186789124 704 + 3.002 2.931 -0.236
ENSG00000156976 E052 1910.2539568 0.0017145134 2.660168e-01 0.94041302 3 186789125 186789897 773 + 3.285 3.276 -0.029