ENSG00000117519

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370206 ENSG00000117519 HEK293_DMSO_6hB HEK293_TMG_6hB CNN3 protein_coding protein_coding 42.05079 57.53028 59.42986 0.695492 2.65606 0.04685841 36.739339 50.152476 48.446291 1.415680 1.677113 -0.04992458 0.87907500 0.8717 0.8163333 -0.05536667 0.95053252 0.03974255 FALSE  
ENST00000487539 ENSG00000117519 HEK293_DMSO_6hB HEK293_TMG_6hB CNN3 protein_coding processed_transcript 42.05079 57.53028 59.42986 0.695492 2.65606 0.04685841 2.828587 3.971771 8.965497 0.388085 1.568729 1.17258162 0.05265833 0.0691 0.1491667 0.08006667 0.03974255 0.03974255 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_DMSO_6hB log2fold_HEK293_DMSO_6hB_HEK293_TMG_6hB
ENSG00000117519 E001 0.0000000       1 94896949 94896956 8 -      
ENSG00000117519 E002 973.9242716 0.0001231482 3.384902e-02 0.92245826 1 94896957 94897867 911 - 3.001 2.977 -0.079
ENSG00000117519 E003 456.5475060 0.0001446738 6.391552e-01 0.95088618 1 94897868 94897946 79 - 2.656 2.664 0.026
ENSG00000117519 E004 455.6224256 0.0001577700 9.386873e-01 0.99527682 1 94897947 94898051 105 - 2.660 2.659 -0.004
ENSG00000117519 E005 305.7200363 0.0002917484 9.426393e-01 0.99574383 1 94898052 94898083 32 - 2.486 2.485 -0.005
ENSG00000117519 E006 562.6378515 0.0001651605 5.365456e-01 0.94041302 1 94899371 94899517 147 - 2.756 2.746 -0.031
ENSG00000117519 E007 537.1445736 0.0001472954 1.116800e-01 0.94041302 1 94901669 94901785 117 - 2.742 2.718 -0.081
ENSG00000117519 E008 3.1761513 0.0155694709 5.588669e-01 0.94131051 1 94901786 94901873 88 - 0.566 0.659 0.409
ENSG00000117519 E009 382.2838322 0.0001966520 5.181738e-01 0.94041302 1 94902121 94902177 57 - 2.589 2.577 -0.039
ENSG00000117519 E010 437.6577998 0.0001520324 6.527893e-01 0.95304965 1 94902178 94902258 81 - 2.645 2.638 -0.025
ENSG00000117519 E011 374.0288505 0.0009051496 9.497071e-01 0.99655978 1 94903122 94903188 67 - 2.576 2.574 -0.007
ENSG00000117519 E012 2.9436712 0.0121338619 3.292914e-01 0.94041302 1 94903189 94903321 133 - 0.670 0.514 -0.699
ENSG00000117519 E013 406.3715933 0.0002468681 3.067330e-02 0.91229825 1 94903403 94903524 122 - 2.591 2.630 0.131
ENSG00000117519 E014 0.1866901 0.0434052464 4.720264e-01   1 94924174 94924447 274 - 0.126 0.000 -8.707
ENSG00000117519 E015 0.3521593 0.0319832284 9.797045e-01   1 94925633 94925808 176 - 0.126 0.122 -0.050
ENSG00000117519 E016 378.7593177 0.0003260069 1.253211e-05 0.05148253 1 94926838 94927223 386 - 2.535 2.620 0.282