ENSG00000105298

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000248420 ENSG00000105298 HEK293_DMSO_6hB HEK293_TMG_6hB CACTIN protein_coding protein_coding 28.26476 16.50782 16.76053 0.9117101 0.9441662 0.02190556 0.3068901 0.0000000 1.567242 0.00000000 0.83391858 7.3012606 0.01781250 0.00000000 0.09796667 0.09796667 0.705384480 0.002075282 FALSE TRUE
ENST00000429344 ENSG00000105298 HEK293_DMSO_6hB HEK293_TMG_6hB CACTIN protein_coding protein_coding 28.26476 16.50782 16.76053 0.9117101 0.9441662 0.02190556 20.2053255 11.6174302 9.551882 1.00617441 1.19872134 -0.2821657 0.70487083 0.70360000 0.56590000 -0.13770000 0.740177946 0.002075282 FALSE TRUE
ENST00000587175 ENSG00000105298 HEK293_DMSO_6hB HEK293_TMG_6hB CACTIN protein_coding retained_intron 28.26476 16.50782 16.76053 0.9117101 0.9441662 0.02190556 1.4661822 0.7215556 1.686167 0.06365097 0.07430000 1.2132392 0.05654583 0.04356667 0.10076667 0.05720000 0.002075282 0.002075282 FALSE TRUE
ENST00000591978 ENSG00000105298 HEK293_DMSO_6hB HEK293_TMG_6hB CACTIN protein_coding retained_intron 28.26476 16.50782 16.76053 0.9117101 0.9441662 0.02190556 1.4843164 1.1801350 1.540075 0.08203722 0.04773829 0.3812128 0.06066667 0.07240000 0.09283333 0.02043333 0.950532522 0.002075282 FALSE FALSE
ENST00000592721 ENSG00000105298 HEK293_DMSO_6hB HEK293_TMG_6hB CACTIN protein_coding protein_coding 28.26476 16.50782 16.76053 0.9117101 0.9441662 0.02190556 2.1996548 0.5116253 0.000000 0.51162535 0.00000000 -5.7049421 0.05837083 0.03453333 0.00000000 -0.03453333 0.950532522 0.002075282 FALSE TRUE
MSTRG.16167.8 ENSG00000105298 HEK293_DMSO_6hB HEK293_TMG_6hB CACTIN protein_coding   28.26476 16.50782 16.76053 0.9117101 0.9441662 0.02190556 1.3221204 1.3653977 1.412249 0.57705657 0.84829006 0.0483248 0.06082083 0.07936667 0.08546667 0.00610000 1.000000000 0.002075282 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_DMSO_6hB log2fold_HEK293_DMSO_6hB_HEK293_TMG_6hB
ENSG00000105298 E001 0.1538588 0.0445209651 6.291505e-01   19 3610645 3610646 2 - 0.000 0.117 9.492
ENSG00000105298 E002 0.1538588 0.0445209651 6.291505e-01   19 3610647 3610649 3 - 0.000 0.117 9.494
ENSG00000105298 E003 0.6385012 0.0230136124 6.574280e-02   19 3610650 3610659 10 - 0.000 0.350 11.494
ENSG00000105298 E004 15.7660342 0.0076179427 1.574196e-02 0.82796636 19 3610660 3610704 45 - 1.074 1.337 0.934
ENSG00000105298 E005 96.1574404 0.9024580391 4.054848e-01 0.94041302 19 3610705 3610966 262 - 1.892 2.059 0.562
ENSG00000105298 E006 43.4428173 0.3728616219 2.834792e-01 0.94041302 19 3610967 3610971 5 - 1.549 1.723 0.592
ENSG00000105298 E007 61.6641855 0.4634336272 2.202879e-01 0.94041302 19 3610972 3611061 90 - 1.660 1.893 0.789
ENSG00000105298 E008 81.7818059 0.7890911598 3.639992e-01 0.94041302 19 3611062 3611228 167 - 1.816 1.994 0.599
ENSG00000105298 E009 72.9856954 0.5740498033 3.629659e-01 0.94041302 19 3611229 3611360 132 - 1.790 1.931 0.472
ENSG00000105298 E010 33.7265325 0.2688196663 2.103254e-01 0.94041302 19 3611361 3611364 4 - 1.413 1.633 0.751
ENSG00000105298 E011 94.6293520 0.9080949915 4.345413e-01 0.94041302 19 3611365 3611567 203 - 1.897 2.045 0.495
ENSG00000105298 E012 140.8367814 1.0843618081 4.747803e-01 0.94041302 19 3611568 3611899 332 - 2.078 2.209 0.437
ENSG00000105298 E013 43.1038391 0.3870572968 3.233690e-01 0.94041302 19 3611900 3611903 4 - 1.560 1.710 0.511
ENSG00000105298 E014 243.9965097 1.3034484869 4.934003e-01 0.94041302 19 3611904 3612413 510 - 2.311 2.450 0.465
ENSG00000105298 E015 76.9905041 0.5647265568 3.009663e-01 0.94041302 19 3613058 3613365 308 - 1.783 1.973 0.641
ENSG00000105298 E016 25.6611430 0.1630019999 1.374841e-01 0.94041302 19 3613464 3613506 43 - 1.277 1.531 0.877
ENSG00000105298 E017 37.1643346 0.2771248819 1.708496e-01 0.94041302 19 3613507 3613586 80 - 1.438 1.683 0.837
ENSG00000105298 E018 5.9920895 0.1003122737 2.573057e-01 0.94041302 19 3614386 3614396 11 - 0.736 0.939 0.793
ENSG00000105298 E019 23.9405911 0.0524690216 5.304762e-02 0.94041302 19 3614397 3614409 13 - 1.249 1.506 0.892
ENSG00000105298 E020 65.3664509 0.0005778657 7.279381e-01 0.96526178 19 3614410 3614494 85 - 1.812 1.839 0.091
ENSG00000105298 E021 88.8909481 0.0379383695 2.253371e-01 0.94041302 19 3614495 3614589 95 - 1.976 1.933 -0.144
ENSG00000105298 E022 69.5990393 0.0730206501 8.036003e-02 0.94041302 19 3614590 3616692 2103 - 1.927 1.761 -0.559
ENSG00000105298 E023 109.1138862 0.0480661217 1.784552e-01 0.94041302 19 3618875 3618989 115 - 2.075 2.008 -0.224
ENSG00000105298 E024 123.7803340 0.0477356272 1.560177e-01 0.94041302 19 3619080 3619242 163 - 2.133 2.059 -0.246
ENSG00000105298 E025 132.7507303 0.0592723237 1.359127e-01 0.94041302 19 3620127 3620272 146 - 2.176 2.074 -0.341
ENSG00000105298 E026 123.7290271 0.0444904751 6.723104e-02 0.94041302 19 3620707 3620802 96 - 2.153 2.036 -0.393
ENSG00000105298 E027 13.1853833 0.0059331108 3.427381e-05 0.08328921 19 3620803 3621028 226 - 1.307 0.941 -1.320
ENSG00000105298 E028 183.0865561 0.0640552987 1.498525e-01 0.94041302 19 3623688 3624162 475 - 2.310 2.219 -0.303
ENSG00000105298 E029 65.4976010 0.0810325089 5.371504e-01 0.94041302 19 3626596 3626815 220 - 1.822 1.823 0.005