ENSG00000256683

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000243644 ENSG00000256683 HEK293_DMSO_2hB HEK293_TMG_2hB ZNF350 protein_coding protein_coding 2.394843 3.32666 2.620363 0.02714801 0.1339849 -0.3431428 1.43051394 1.8064702 2.0407710 0.0464842 0.06001287 0.1750288 0.60875000 0.5429000 0.78306667 0.24016667 9.234337e-02 1.352057e-05 FALSE TRUE
ENST00000594929 ENSG00000256683 HEK293_DMSO_2hB HEK293_TMG_2hB ZNF350 protein_coding protein_coding 2.394843 3.32666 2.620363 0.02714801 0.1339849 -0.3431428 0.09379375 0.0549591 0.1166264 0.0549591 0.06396173 0.9629746 0.05637083 0.0163000 0.04243333 0.02613333 9.311750e-01 1.352057e-05   FALSE
ENST00000597788 ENSG00000256683 HEK293_DMSO_2hB HEK293_TMG_2hB ZNF350 protein_coding protein_coding 2.394843 3.32666 2.620363 0.02714801 0.1339849 -0.3431428 0.18433416 0.3875447 0.2981241 0.2199446 0.08352965 -0.3676057 0.07742917 0.1174333 0.11263333 -0.00480000 1.000000e+00 1.352057e-05   FALSE
ENST00000599258 ENSG00000256683 HEK293_DMSO_2hB HEK293_TMG_2hB ZNF350 protein_coding processed_transcript 2.394843 3.32666 2.620363 0.02714801 0.1339849 -0.3431428 0.34573847 0.0000000 0.0000000 0.0000000 0.00000000 0.0000000 0.14130833 0.0000000 0.00000000 0.00000000   1.352057e-05   FALSE
ENST00000600703 ENSG00000256683 HEK293_DMSO_2hB HEK293_TMG_2hB ZNF350 protein_coding processed_transcript 2.394843 3.32666 2.620363 0.02714801 0.1339849 -0.3431428 0.22675513 0.9560768 0.0000000 0.2710697 0.00000000 -6.5940660 0.07427083 0.2867000 0.00000000 -0.28670000 1.352057e-05 1.352057e-05 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000256683 E001 68.4876260 0.0003761321 0.0002855949 0.07074992 19 51964340 51965855 1516 - 1.901 1.782 -0.402
ENSG00000256683 E002 27.1310268 0.0007855364 0.5093013269 0.96934669 19 51965856 51966214 359 - 1.464 1.429 -0.124
ENSG00000256683 E003 0.3193150 0.0282845720 0.2537372730   19 51967288 51967400 113 - 0.000 0.206 10.596
ENSG00000256683 E004 0.0000000       19 51968479 51968577 99 -      
ENSG00000256683 E005 7.7284175 0.0063975587 0.0659268866   19 51968578 51968613 36 - 0.807 1.030 0.845
ENSG00000256683 E006 7.4330698 0.0026023063 0.2987441518   19 51968614 51968629 16 - 0.853 0.978 0.472
ENSG00000256683 E007 9.7759322 0.0019121173 0.3137652082   19 51968630 51968658 29 - 0.968 1.077 0.399
ENSG00000256683 E008 11.2086984 0.0018105318 0.0027505897 0.23436490 19 51968659 51968673 15 - 0.895 1.201 1.120
ENSG00000256683 E009 16.7990425 0.0236229703 0.2492763640 0.91805531 19 51969005 51969131 127 - 1.177 1.300 0.433
ENSG00000256683 E010 0.4936172 0.0249708937 0.1159223302   19 51969132 51969145 14 - 0.000 0.281 11.169
ENSG00000256683 E011 2.0468222 0.0111902352 0.0404649021   19 51972967 51974345 1379 - 0.628 0.281 -1.832
ENSG00000256683 E012 22.8229931 0.0009057194 0.6299705828 0.98026574 19 51974346 51974531 186 - 1.355 1.393 0.131
ENSG00000256683 E013 3.2635345 0.0129879970 0.6424901416   19 51974678 51974853 176 - 0.589 0.667 0.336
ENSG00000256683 E014 0.5216514 0.1666903923 0.7136676592   19 51981925 51982031 107 - 0.134 0.207 0.761
ENSG00000256683 E015 1.1187979 0.0129091073 0.0816603719   19 51984048 51984169 122 - 0.134 0.450 2.337
ENSG00000256683 E016 0.5067967 0.0234033226 0.4654516410   19 51986510 51986769 260 - 0.236 0.115 -1.247
ENSG00000256683 E017 12.0838002 0.0247202819 0.5670581745 0.97520819 19 51986770 51986856 87 - 1.146 1.088 -0.210