ENSG00000197461

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000400761 ENSG00000197461 HEK293_DMSO_2hB HEK293_TMG_2hB PDGFA protein_coding protein_coding 3.193151 3.436272 2.815507 0.1400885 0.1619584 -0.286527 0.1406908 0.4973008 0.000000 0.27301489 0.000000 -5.66476950 0.03557917 0.1389333 0.0000000 -0.1389333 0.616655918 0.001026228 FALSE FALSE
ENST00000402802 ENSG00000197461 HEK293_DMSO_2hB HEK293_TMG_2hB PDGFA protein_coding protein_coding 3.193151 3.436272 2.815507 0.1400885 0.1619584 -0.286527 2.8787669 2.6440653 2.783853 0.08679434 0.147274 0.07405216 0.91034583 0.7736333 0.9893667 0.2157333 0.001026228 0.001026228 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000197461 E001 68.2821378 0.0003439616 0.0357151996 0.62960089 7 497258 498308 1051 - 1.880 1.803 -0.260
ENSG00000197461 E002 50.3387275 0.0003830386 0.0002228444 0.05988074 7 498309 498492 184 - 1.789 1.623 -0.564
ENSG00000197461 E003 32.6486402 0.0005580825 0.0102003665 0.41597958 7 498493 498574 82 - 1.597 1.451 -0.501
ENSG00000197461 E004 1.0868477 0.0168014624 0.3310562516   7 500431 500499 69 - 0.231 0.408 1.157
ENSG00000197461 E005 0.8632642 0.5843798926 0.7899914558   7 500778 501115 338 - 0.314 0.214 -0.737
ENSG00000197461 E006 34.1710773 0.0005778083 0.7570202615 0.98848087 7 501116 501242 127 - 1.552 1.538 -0.050
ENSG00000197461 E007 35.7273986 0.0005573600 0.0739195908 0.75641339 7 510809 510996 188 - 1.507 1.610 0.351
ENSG00000197461 E008 14.3617440 0.0013559365 0.2018121499 0.89564147 7 512351 512356 6 - 1.122 1.237 0.409
ENSG00000197461 E009 24.9241527 0.0007820906 0.2427383692 0.91482002 7 512357 512444 88 - 1.369 1.451 0.281
ENSG00000197461 E010 14.2064670 0.0495354444 0.3533709279 0.95019149 7 512445 512455 11 - 1.123 1.234 0.395
ENSG00000197461 E011 0.3170746 0.0283277858 0.2342107939   7 512456 513467 1012 - 0.000 0.211 9.180
ENSG00000197461 E012 20.8427290 0.0009588695 0.0052869338 0.31743632 7 517394 517490 97 - 1.215 1.426 0.736
ENSG00000197461 E013 0.0000000       7 518419 518508 90 -      
ENSG00000197461 E014 19.0156364 0.0010576812 0.0353932138 0.62760615 7 518939 519068 130 - 1.206 1.372 0.582
ENSG00000197461 E015 13.8634946 0.0013561797 0.1524494923 0.85819218 7 519069 519409 341 - 1.099 1.229 0.466
ENSG00000197461 E016 1.5215006 0.0268112099 0.0239880212   7 519960 520296 337 - 0.130 0.545 2.843