ENSG00000186866

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000331343 ENSG00000186866 HEK293_DMSO_2hB HEK293_TMG_2hB POFUT2 protein_coding protein_coding 23.69575 25.05869 22.08307 1.362702 0.6386953 -0.1822931 1.5509533 1.1271150 1.427029 0.1034323 0.02740593 0.3377106 0.06623750 0.04556667 0.06476667 0.0192 0.6532615225 0.0003349452 FALSE TRUE
ENST00000349485 ENSG00000186866 HEK293_DMSO_2hB HEK293_TMG_2hB POFUT2 protein_coding protein_coding 23.69575 25.05869 22.08307 1.362702 0.6386953 -0.1822931 15.1101425 14.5242278 12.899586 0.8644167 0.66290120 -0.1710116 0.63739167 0.57963333 0.58533333 0.0057 1.0000000000 0.0003349452 FALSE TRUE
ENST00000476653 ENSG00000186866 HEK293_DMSO_2hB HEK293_TMG_2hB POFUT2 protein_coding processed_transcript 23.69575 25.05869 22.08307 1.362702 0.6386953 -0.1822931 1.4509080 0.9155005 2.697416 0.1440481 0.24377542 1.5486107 0.06182500 0.03726667 0.12176667 0.0845 0.0003349452 0.0003349452 FALSE FALSE
ENST00000493811 ENSG00000186866 HEK293_DMSO_2hB HEK293_TMG_2hB POFUT2 protein_coding processed_transcript 23.69575 25.05869 22.08307 1.362702 0.6386953 -0.1822931 0.5680575 2.8841484 0.748629 1.4510358 0.74862905 -1.9316724 0.02284167 0.11426667 0.03216667 -0.0821 0.9311750285 0.0003349452 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000186866 E001 1.620843 1.022712e-02 0.8402859275   21 45263928 45263928 1 - 0.441 0.407 -0.182
ENSG00000186866 E002 1.799213 9.719426e-03 0.9310644032   21 45263929 45263934 6 - 0.441 0.457 0.081
ENSG00000186866 E003 22.981218 8.368440e-04 0.7516360965 0.98824255 21 45263935 45263978 44 - 1.391 1.369 -0.078
ENSG00000186866 E004 547.712858 8.405393e-05 0.5731816760 0.97590783 21 45263979 45265373 1395 - 2.743 2.736 -0.025
ENSG00000186866 E005 74.283446 9.525310e-03 0.2585634050 0.92038226 21 45265374 45265399 26 - 1.837 1.906 0.231
ENSG00000186866 E006 220.206662 1.053933e-04 0.4181214481 0.95869370 21 45265400 45265635 236 - 2.334 2.354 0.064
ENSG00000186866 E007 18.421081 3.743000e-02 0.6019893891 0.97765468 21 45265636 45266021 386 - 1.317 1.254 -0.222
ENSG00000186866 E008 19.492230 1.769964e-02 0.5726692912 0.97584025 21 45266022 45266227 206 - 1.338 1.281 -0.199
ENSG00000186866 E009 50.972209 4.522012e-04 0.0001151133 0.03920758 21 45266228 45267525 1298 - 1.804 1.614 -0.646
ENSG00000186866 E010 8.821928 2.126448e-03 0.2601693250   21 45267526 45267589 64 - 1.050 0.927 -0.454
ENSG00000186866 E011 132.251392 1.526551e-04 0.4841278347 0.96627805 21 45267590 45267697 108 - 2.136 2.115 -0.070
ENSG00000186866 E012 69.087803 2.622010e-04 0.1989174045 0.89366309 21 45267698 45267713 16 - 1.873 1.819 -0.181
ENSG00000186866 E013 64.541750 2.782343e-04 0.5556720266 0.97398445 21 45269839 45269840 2 - 1.830 1.805 -0.085
ENSG00000186866 E014 71.972349 2.519813e-04 0.4906282528 0.96666067 21 45269841 45269850 10 - 1.877 1.849 -0.095
ENSG00000186866 E015 149.240822 1.382076e-04 0.6369514127 0.98094177 21 45269851 45270019 169 - 2.169 2.183 0.046
ENSG00000186866 E016 4.135767 4.812529e-03 0.4990517343   21 45270020 45270094 75 - 0.656 0.753 0.403
ENSG00000186866 E017 159.308207 1.539247e-04 0.3432466587 0.94854959 21 45277017 45277142 126 - 2.190 2.217 0.089
ENSG00000186866 E018 5.084767 3.971204e-03 0.2179403428   21 45277233 45277773 541 - 0.866 0.702 -0.651
ENSG00000186866 E019 142.256953 1.793754e-04 0.6487188926 0.98229974 21 45278103 45278169 67 - 2.150 2.164 0.046
ENSG00000186866 E020 104.978204 3.614949e-04 0.7690228191 0.98990885 21 45282349 45282368 20 - 2.021 2.032 0.036
ENSG00000186866 E021 102.845756 1.888931e-04 0.9476662001 0.99853292 21 45282369 45282398 30 - 2.015 2.018 0.009
ENSG00000186866 E022 122.935709 1.865407e-04 0.7777708082 0.99048610 21 45282399 45282459 61 - 2.090 2.099 0.031
ENSG00000186866 E023 7.331249 2.524159e-03 0.6802397354   21 45282804 45282938 135 - 0.942 0.894 -0.181
ENSG00000186866 E024 10.409955 1.802443e-03 0.8974710406 0.99635313 21 45283352 45283382 31 - 1.050 1.063 0.048
ENSG00000186866 E025 185.612546 1.248224e-04 0.4679472327 0.96327971 21 45283383 45283527 145 - 2.261 2.280 0.063
ENSG00000186866 E026 4.661479 4.065470e-03 0.9622016765   21 45285269 45285328 60 - 0.747 0.753 0.026
ENSG00000186866 E027 10.073902 1.917641e-03 0.0009737854 0.14254521 21 45285483 45285677 195 - 1.189 0.839 -1.290
ENSG00000186866 E028 233.858239 1.221782e-04 0.3255763558 0.94289833 21 45285678 45285928 251 - 2.360 2.382 0.075
ENSG00000186866 E029 103.247685 2.372120e-04 0.1708875096 0.87449556 21 45287741 45287898 158 - 1.994 2.042 0.160