ENSG00000162849

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366518 ENSG00000162849 HEK293_DMSO_2hB HEK293_TMG_2hB KIF26B protein_coding protein_coding 0.6323529 1.051707 0.8951131 0.1009438 0.01770679 -0.2302154 0.07772491 0.0000000 0.0000000 0.000000000 0.00000000 0.0000000 0.10751667 0.0000000 0 0.0000000   0.04393882 FALSE TRUE
ENST00000407071 ENSG00000162849 HEK293_DMSO_2hB HEK293_TMG_2hB KIF26B protein_coding protein_coding 0.6323529 1.051707 0.8951131 0.1009438 0.01770679 -0.2302154 0.50779169 0.8099180 0.8951131 0.007666685 0.01770679 0.1426183 0.83031667 0.7848333 1 0.2151667 0.04393882 0.04393882 FALSE TRUE
ENST00000483253 ENSG00000162849 HEK293_DMSO_2hB HEK293_TMG_2hB KIF26B protein_coding retained_intron 0.6323529 1.051707 0.8951131 0.1009438 0.01770679 -0.2302154 0.02989443 0.2082088 0.0000000 0.107798430 0.00000000 -4.4476375 0.03290833 0.1802667 0 -0.1802667 0.61425578 0.04393882 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000162849 E001 17.6507383 0.0017867252 0.2325805967 0.91118911 1 245154985 245155487 503 + 1.313 1.216 -0.340
ENSG00000162849 E002 10.5531423 0.0019407064 0.5380600359 0.97255154 1 245156282 245156683 402 + 1.033 1.094 0.224
ENSG00000162849 E003 0.0000000       1 245353678 245353916 239 +      
ENSG00000162849 E004 20.8366488 0.0009437137 0.3620593070 0.95123488 1 245366834 245367367 534 + 1.371 1.304 -0.233
ENSG00000162849 E005 0.0000000       1 245370576 245370694 119 +      
ENSG00000162849 E006 0.4620873 0.0258130906 0.0798930221   1 245371421 245371717 297 + 0.000 0.302 10.544
ENSG00000162849 E007 9.1597102 0.0032847404 0.4920263905   1 245419579 245419745 167 + 1.046 0.973 -0.268
ENSG00000162849 E008 0.1730468 0.0404413065 0.5026085046   1 245511002 245511128 127 + 0.123 0.000 -9.765
ENSG00000162849 E009 7.0441132 0.0033783494 0.7193352028   1 245540767 245540950 184 + 0.929 0.887 -0.159
ENSG00000162849 E010 10.0446103 0.0018829720 0.0268380631 0.58372899 1 245602577 245602783 207 + 1.137 0.906 -0.850
ENSG00000162849 E011 8.9088478 0.0791685586 0.8465338505   1 245607651 245607744 94 + 1.022 0.959 -0.231
ENSG00000162849 E012 11.0863092 0.0043314281 0.4718850990 0.96344252 1 245609266 245609528 263 + 1.046 1.117 0.259
ENSG00000162849 E013 10.2457528 0.0021337072 0.5219792765 0.97014308 1 245611793 245611976 184 + 1.082 1.017 -0.237
ENSG00000162849 E014 0.0000000       1 245646092 245646120 29 +      
ENSG00000162849 E015 8.1733170 0.0265085186 0.3586518841   1 245646121 245646280 160 + 1.020 0.903 -0.434
ENSG00000162849 E016 9.6676105 0.0377750610 0.9449224815   1 245684233 245684395 163 + 1.019 1.042 0.084
ENSG00000162849 E017 89.6853001 0.0002936506 0.0004823746 0.09469201 1 245685405 245688807 3403 + 1.897 2.012 0.386
ENSG00000162849 E018 11.1402501 0.0018583006 0.3295621617 0.94432471 1 245698106 245698308 203 + 1.033 1.128 0.346
ENSG00000162849 E019 10.0767714 0.0018488335 0.9907159239 0.99943420 1 245698887 245699037 151 + 1.046 1.045 -0.004
ENSG00000162849 E020 137.9776912 0.0001928602 0.1781496437 0.87981038 1 245702458 245709432 6975 + 2.158 2.125 -0.109