ENSG00000134278

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000410092 ENSG00000134278 HEK293_DMSO_2hB HEK293_TMG_2hB SPIRE1 protein_coding protein_coding 8.059124 14.75508 10.55155 1.23106 0.3946022 -0.4833672 5.2786781 6.8653571 7.8987110 0.2226158 0.01812868 0.2020079 0.71815417 0.46950000 0.75066667 0.28116667 4.645876e-05 4.645876e-05 FALSE TRUE
ENST00000449797 ENSG00000134278 HEK293_DMSO_2hB HEK293_TMG_2hB SPIRE1 protein_coding protein_coding 8.059124 14.75508 10.55155 1.23106 0.3946022 -0.4833672 0.1704922 0.9382486 0.0000000 0.9382486 0.00000000 -6.5671934 0.01088750 0.05526667 0.00000000 -0.05526667 9.472275e-01 4.645876e-05 FALSE FALSE
ENST00000498803 ENSG00000134278 HEK293_DMSO_2hB HEK293_TMG_2hB SPIRE1 protein_coding retained_intron 8.059124 14.75508 10.55155 1.23106 0.3946022 -0.4833672 0.3579115 1.5671161 0.3527375 0.2999795 0.18949303 -2.1202909 0.03855000 0.10576667 0.03313333 -0.07263333 7.836184e-01 4.645876e-05 FALSE TRUE
ENST00000588236 ENSG00000134278 HEK293_DMSO_2hB HEK293_TMG_2hB SPIRE1 protein_coding retained_intron 8.059124 14.75508 10.55155 1.23106 0.3946022 -0.4833672 0.5704791 2.4206480 0.2056265 0.4704292 0.20562648 -3.4947347 0.04994583 0.16103333 0.01813333 -0.14290000 3.513586e-01 4.645876e-05 FALSE FALSE
MSTRG.15521.6 ENSG00000134278 HEK293_DMSO_2hB HEK293_TMG_2hB SPIRE1 protein_coding   8.059124 14.75508 10.55155 1.23106 0.3946022 -0.4833672 0.8381788 1.7432122 1.0881628 0.9662786 0.55227735 -0.6749086 0.07253750 0.12600000 0.10053333 -0.02546667 1.000000e+00 4.645876e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000134278 E001 0.0000000       18 12446512 12446512 1 -      
ENSG00000134278 E002 616.6708842 0.0002646345 0.0002179934 0.05918363 18 12446513 12449503 2991 - 2.816 2.763 -0.175
ENSG00000134278 E003 27.2622746 0.0008514313 0.9988083948 0.99989764 18 12449504 12449505 2 - 1.453 1.450 -0.010
ENSG00000134278 E004 27.5743044 0.0007031263 0.6486454868 0.98229067 18 12449506 12449508 3 - 1.443 1.470 0.093
ENSG00000134278 E005 50.5996554 0.0003613427 0.9156881109 0.99692361 18 12449509 12449572 64 - 1.717 1.709 -0.027
ENSG00000134278 E006 68.4050840 0.0042797067 0.3990847574 0.95610456 18 12449573 12449665 93 - 1.818 1.860 0.139
ENSG00000134278 E007 40.8223720 0.0008822444 0.5521890907 0.97375634 18 12449666 12449667 2 - 1.603 1.633 0.103
ENSG00000134278 E008 94.5426005 0.0002452055 0.2167859777 0.90373757 18 12449668 12449809 142 - 1.959 2.000 0.140
ENSG00000134278 E009 74.6871946 0.0002518955 0.1543911561 0.85934133 18 12449810 12449896 87 - 1.850 1.905 0.184
ENSG00000134278 E010 99.7718328 0.0002352185 0.0139229550 0.46782564 18 12452255 12452391 137 - 1.960 2.043 0.280
ENSG00000134278 E011 0.8004679 0.0171410268 0.6686485569   18 12452392 12452443 52 - 0.222 0.297 0.556
ENSG00000134278 E012 58.7567621 0.0003184514 0.0205762103 0.53285337 18 12452485 12452512 28 - 1.723 1.825 0.348
ENSG00000134278 E013 5.6289646 0.0138103476 0.2103033398   18 12452513 12452610 98 - 0.728 0.897 0.664
ENSG00000134278 E014 72.5221200 0.0003980493 0.6256988612 0.97959912 18 12453068 12453138 71 - 1.858 1.876 0.058
ENSG00000134278 E015 80.0669417 0.0002283261 0.5214387285 0.97003046 18 12454346 12454483 138 - 1.897 1.919 0.074
ENSG00000134278 E016 0.6314884 0.0223562025 0.3208106495   18 12454484 12454506 23 - 0.125 0.297 1.556
ENSG00000134278 E017 5.9798087 0.0030693254 0.1062491276   18 12459755 12459928 174 - 0.728 0.932 0.796
ENSG00000134278 E018 80.1172996 0.0002273038 0.7180492156 0.98679464 18 12463351 12463493 143 - 1.917 1.900 -0.057
ENSG00000134278 E019 57.2965109 0.0003489935 0.6338749784 0.98052624 18 12464868 12464958 91 - 1.754 1.774 0.065
ENSG00000134278 E020 75.7775371 0.0002710918 0.8062320249 0.99111030 18 12479699 12479871 173 - 1.880 1.887 0.024
ENSG00000134278 E021 2.4297083 0.0348919812 0.2385462925   18 12485959 12486000 42 - 0.427 0.631 0.970
ENSG00000134278 E022 48.1203889 0.0010129433 0.1386572870 0.84698143 18 12493072 12493138 67 - 1.654 1.728 0.250
ENSG00000134278 E023 56.4354671 0.0022415878 0.7371088520 0.98787069 18 12493139 12493201 63 - 1.752 1.766 0.049
ENSG00000134278 E024 61.3200734 0.0071380080 0.7732199630 0.99033428 18 12496016 12496102 87 - 1.803 1.786 -0.058
ENSG00000134278 E025 38.5339760 0.0085024169 0.2084444602 0.89952494 18 12506477 12506495 19 - 1.641 1.552 -0.305
ENSG00000134278 E026 91.0894982 0.0027719567 0.8153967019 0.99158961 18 12506496 12506641 146 - 1.959 1.967 0.026
ENSG00000134278 E027 67.1537883 0.0004386103 0.8988084924 0.99638518 18 12512454 12512512 59 - 1.829 1.832 0.009
ENSG00000134278 E028 40.5391724 0.0004788531 0.7743215851 0.99033428 18 12512513 12512531 19 - 1.610 1.623 0.044
ENSG00000134278 E029 0.1450127 0.0420237927 0.4932610275   18 12516071 12516225 155 - 0.001 0.123 8.063
ENSG00000134278 E030 32.7958381 0.0005502903 0.9551947071 0.99881842 18 12535476 12535477 2 - 1.528 1.529 0.002
ENSG00000134278 E031 92.6467924 0.0002154457 0.7148347636 0.98673520 18 12535478 12535601 124 - 1.965 1.975 0.035
ENSG00000134278 E032 103.3461579 0.0003198248 0.4800679759 0.96565878 18 12546674 12546904 231 - 2.030 2.003 -0.092
ENSG00000134278 E033 0.0000000       18 12588376 12588419 44 -      
ENSG00000134278 E034 25.5800997 0.0013877736 0.4842685027 0.96627805 18 12635062 12635096 35 - 1.448 1.397 -0.177
ENSG00000134278 E035 0.0000000       18 12636324 12636340 17 -      
ENSG00000134278 E036 0.0000000       18 12647795 12647865 71 -      
ENSG00000134278 E037 0.0000000       18 12653001 12653085 85 -      
ENSG00000134278 E038 0.0000000       18 12653384 12653440 57 -      
ENSG00000134278 E039 0.0000000       18 12656575 12656650 76 -      
ENSG00000134278 E040 0.0000000       18 12656651 12656736 86 -      
ENSG00000134278 E041 63.8008210 0.0109838609 0.1625713108 0.86698523 18 12657530 12658107 578 - 1.853 1.760 -0.313