ENSG00000103326

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000219611 ENSG00000103326 HEK293_DMSO_2hB HEK293_TMG_2hB CAPN15 protein_coding protein_coding 42.40197 24.9924 25.07245 0.7228359 0.6741258 0.00461175 7.222670 5.9298470 4.600008 0.20863198 0.1058795 -0.36565651 0.17060417 0.23776667 0.18383333 -0.053933333 6.142558e-01 7.041887e-16 FALSE  
ENST00000565010 ENSG00000103326 HEK293_DMSO_2hB HEK293_TMG_2hB CAPN15 protein_coding retained_intron 42.40197 24.9924 25.07245 0.7228359 0.6741258 0.00461175 11.734318 5.3051941 5.806285 0.35921463 0.5769787 0.12997558 0.26151667 0.21180000 0.23066667 0.018866667 9.311750e-01 7.041887e-16 FALSE  
ENST00000568402 ENSG00000103326 HEK293_DMSO_2hB HEK293_TMG_2hB CAPN15 protein_coding processed_transcript 42.40197 24.9924 25.07245 0.7228359 0.6741258 0.00461175 5.719156 1.1352307 2.671480 0.08212896 0.1283468 1.22739133 0.13850417 0.04563333 0.10646667 0.060833333 7.312497e-05 7.041887e-16 FALSE  
MSTRG.11722.10 ENSG00000103326 HEK293_DMSO_2hB HEK293_TMG_2hB CAPN15 protein_coding   42.40197 24.9924 25.07245 0.7228359 0.6741258 0.00461175 2.284252 0.6510896 1.262311 0.46167862 0.5646478 0.94453375 0.05238333 0.02673333 0.05100000 0.024266667 9.311750e-01 7.041887e-16 FALSE  
MSTRG.11722.2 ENSG00000103326 HEK293_DMSO_2hB HEK293_TMG_2hB CAPN15 protein_coding   42.40197 24.9924 25.07245 0.7228359 0.6741258 0.00461175 6.754861 6.4241903 5.894498 0.63211926 0.8107548 -0.12394420 0.16745000 0.25800000 0.23573333 -0.022266667 9.455989e-01 7.041887e-16 FALSE  
MSTRG.11722.5 ENSG00000103326 HEK293_DMSO_2hB HEK293_TMG_2hB CAPN15 protein_coding   42.40197 24.9924 25.07245 0.7228359 0.6741258 0.00461175 2.131247 1.1102835 1.049027 0.32268141 0.2224594 -0.08112406 0.05156667 0.04413333 0.04200000 -0.002133333 1.000000e+00 7.041887e-16 FALSE  
MSTRG.11722.8 ENSG00000103326 HEK293_DMSO_2hB HEK293_TMG_2hB CAPN15 protein_coding   42.40197 24.9924 25.07245 0.7228359 0.6741258 0.00461175 1.964410 2.5457851 2.053430 0.67423403 0.4365680 -0.30872231 0.05202500 0.10066667 0.08126667 -0.019400000 9.312298e-01 7.041887e-16 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000103326 E001 1.156366 1.282996e-02 0.6486357138   16 527712 527716 5 + 0.374 0.293 -0.507
ENSG00000103326 E002 1.980237 9.515889e-03 0.9144439029   16 527717 527725 9 + 0.484 0.466 -0.091
ENSG00000103326 E003 2.980381 8.493587e-02 0.4200153584   16 527726 527728 3 + 0.676 0.512 -0.735
ENSG00000103326 E004 7.518875 1.193910e-02 0.0876287786   16 527729 527747 19 + 1.024 0.810 -0.809
ENSG00000103326 E005 16.046710 2.452336e-02 0.1127251773 0.82101635 16 527748 527777 30 + 1.311 1.133 -0.629
ENSG00000103326 E006 19.096615 2.923590e-02 0.1267061260 0.83718059 16 527778 527815 38 + 1.378 1.210 -0.589
ENSG00000103326 E007 18.772247 2.145293e-02 0.1326582104 0.84191287 16 527816 527822 7 + 1.365 1.209 -0.548
ENSG00000103326 E008 35.581546 3.124105e-02 0.6469177721 0.98214575 16 527823 527958 136 + 1.582 1.541 -0.140
ENSG00000103326 E009 43.659588 3.794665e-03 0.3928590301 0.95573686 16 527959 528029 71 + 1.623 1.674 0.173
ENSG00000103326 E010 53.190923 9.912724e-03 0.7415025971 0.98807258 16 533946 533998 53 + 1.723 1.743 0.066
ENSG00000103326 E011 39.207376 5.532019e-04 0.0199227648 0.52850323 16 536029 536142 114 + 1.533 1.665 0.448
ENSG00000103326 E012 6.726954 2.778817e-03 0.0789119656   16 536862 536895 34 + 0.980 0.764 -0.828
ENSG00000103326 E013 28.401826 6.381276e-04 0.6779032246 0.98442711 16 537134 537298 165 + 1.483 1.456 -0.092
ENSG00000103326 E014 13.109009 1.445677e-03 0.6967367256 0.98579096 16 537299 537386 88 + 1.166 1.131 -0.128
ENSG00000103326 E015 10.443613 2.545667e-03 0.9000930098 0.99638518 16 537387 537435 49 + 1.064 1.051 -0.047
ENSG00000103326 E016 158.722017 1.426667e-04 0.0005073242 0.09754536 16 537436 539858 2423 + 2.250 2.153 -0.324
ENSG00000103326 E017 10.326305 1.819365e-03 0.9064397527 0.99664597 16 540093 540104 12 + 1.051 1.064 0.045
ENSG00000103326 E018 22.304043 1.029073e-02 0.4891875735 0.96648244 16 540105 540371 267 + 1.397 1.339 -0.202
ENSG00000103326 E019 360.889802 4.916773e-04 0.3436879465 0.94854959 16 546817 547558 742 + 2.549 2.568 0.064
ENSG00000103326 E020 248.378693 9.418618e-04 0.0478756704 0.67968690 16 547559 548031 473 + 2.369 2.420 0.169
ENSG00000103326 E021 199.499093 1.661146e-04 0.8429433175 0.99347532 16 548032 548287 256 + 2.301 2.306 0.017
ENSG00000103326 E022 174.291866 1.180169e-03 0.8168034886 0.99164253 16 548993 549201 209 + 2.241 2.249 0.026
ENSG00000103326 E023 149.071867 1.687556e-04 0.3542275361 0.95019149 16 549288 549471 184 + 2.163 2.190 0.090
ENSG00000103326 E024 51.325642 1.333183e-02 0.7142070427 0.98658832 16 549615 549620 6 + 1.733 1.706 -0.093
ENSG00000103326 E025 96.264129 6.595866e-03 0.9598582944 0.99897591 16 549621 549692 72 + 1.991 1.985 -0.019
ENSG00000103326 E026 166.282874 1.426107e-04 0.5258986520 0.97069266 16 549693 549838 146 + 2.232 2.215 -0.058
ENSG00000103326 E027 183.174605 4.549853e-04 0.9905736098 0.99943420 16 551302 551427 126 + 2.265 2.266 0.002
ENSG00000103326 E028 198.834011 1.362246e-03 0.3113536362 0.93917153 16 551512 551664 153 + 2.286 2.317 0.104
ENSG00000103326 E029 150.490718 1.509081e-04 0.6542702929 0.98283167 16 552051 552212 162 + 2.175 2.188 0.044
ENSG00000103326 E030 1.722583 2.274034e-01 0.6786080865   16 552297 552300 4 + 0.375 0.472 0.520
ENSG00000103326 E031 182.494919 6.108698e-04 0.4472140428 0.96104157 16 552301 552530 230 + 2.252 2.273 0.070
ENSG00000103326 E032 22.470505 8.908087e-04 0.8412363533 0.99347532 16 552531 552604 74 + 1.378 1.364 -0.050
ENSG00000103326 E033 204.549959 1.153093e-04 0.7645795672 0.98961948 16 552605 552754 150 + 2.309 2.316 0.025
ENSG00000103326 E034 124.890889 1.555583e-04 0.3578030747 0.95118612 16 552755 552771 17 + 2.116 2.087 -0.096
ENSG00000103326 E035 262.027548 8.669046e-05 0.5409061478 0.97285167 16 552863 553041 179 + 2.414 2.427 0.044
ENSG00000103326 E036 73.330835 1.651702e-03 0.5565221722 0.97415733 16 553339 553342 4 + 1.886 1.860 -0.087
ENSG00000103326 E037 725.981319 5.567646e-05 0.7748080769 0.99033428 16 553343 554553 1211 + 2.863 2.860 -0.011
ENSG00000103326 E038 57.633931 3.801273e-04 0.0629546517 0.73017398 16 554554 554636 83 + 1.810 1.724 -0.291