ENSG00000100650

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000553369 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding protein_coding 160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 14.587919 19.548667 25.059841 1.0679085 4.0304368 0.35814480 0.08243750 0.09033333 0.12390000 0.033566667 8.774091e-01 1.933326e-19 FALSE FALSE
ENST00000553548 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding protein_coding 160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 2.012449 11.682973 0.000000 3.7926469 0.0000000 -10.19142609 0.01026250 0.05336667 0.00000000 -0.053366667 1.933326e-19 1.933326e-19 FALSE FALSE
ENST00000554465 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding retained_intron 160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 10.697253 11.059428 13.760661 0.4583308 0.1668674 0.31501716 0.06340833 0.05100000 0.06823333 0.017233333 1.280094e-02 1.933326e-19 FALSE FALSE
ENST00000556184 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding retained_intron 160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 16.243255 17.809774 17.058600 1.2852195 0.8219450 -0.06213438 0.10289583 0.08230000 0.08473333 0.002433333 1.000000e+00 1.933326e-19 FALSE TRUE
ENST00000556330 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding retained_intron 160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 8.064813 6.848804 6.010982 0.2466835 0.2937901 -0.18795814 0.05114583 0.03163333 0.02983333 -0.001800000 9.392728e-01 1.933326e-19 FALSE TRUE
ENST00000557154 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding protein_coding 160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 22.560478 26.632797 23.894768 4.0843089 1.6761341 -0.15644717 0.15282083 0.12336667 0.11856667 -0.004800000 1.000000e+00 1.933326e-19 FALSE TRUE
MSTRG.9806.11 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding   160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 11.615273 7.082875 10.166100 0.1707120 0.9062706 0.52074229 0.07587500 0.03273333 0.05030000 0.017566667 7.011442e-02 1.933326e-19 FALSE TRUE
MSTRG.9806.4 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding   160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 25.739262 48.540506 37.734994 2.3512754 2.4552490 -0.36320102 0.15831667 0.22403333 0.18733333 -0.036700000 8.295235e-01 1.933326e-19 TRUE TRUE
MSTRG.9806.5 ENSG00000100650 HEK293_DMSO_2hB HEK293_TMG_2hB SRSF5 protein_coding   160.3875 216.7217 201.6841 6.09595 3.07358 -0.1037413 29.543934 46.793998 44.356258 8.1675592 6.6177822 -0.07716889 0.18350417 0.21623333 0.22036667 0.004133333 1.000000e+00 1.933326e-19 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000100650 E001 0.0000000       14 69726900 69727191 292 +      
ENSG00000100650 E002 0.0000000       14 69766114 69766467 354 +      
ENSG00000100650 E003 0.0000000       14 69766468 69766728 261 +      
ENSG00000100650 E004 0.6644237 1.791180e-02 3.455029e-01   14 69766729 69767092 364 + 0.128 0.292 1.483
ENSG00000100650 E005 2.9711235 5.998617e-03 2.456445e-02   14 69767093 69767111 19 + 0.375 0.738 1.706
ENSG00000100650 E006 5.2629688 3.427595e-03 7.834449e-03   14 69767112 69767115 4 + 0.573 0.938 1.483
ENSG00000100650 E007 51.3619626 1.632958e-02 7.525381e-02 0.75872144 14 69767116 69767141 26 + 1.638 1.788 0.506
ENSG00000100650 E008 167.3962111 1.424593e-03 6.304602e-02 0.73032573 14 69767142 69767156 15 + 2.196 2.257 0.204
ENSG00000100650 E009 592.5745194 6.412095e-05 1.237812e-01 0.83420614 14 69767157 69767171 15 + 2.763 2.785 0.073
ENSG00000100650 E010 643.1147085 1.966847e-04 1.472555e-01 0.85361276 14 69767172 69767174 3 + 2.799 2.820 0.071
ENSG00000100650 E011 666.3126705 2.351725e-04 1.028132e-01 0.80763780 14 69767175 69767181 7 + 2.813 2.837 0.080
ENSG00000100650 E012 726.7197417 4.963944e-04 3.161208e-01 0.94034912 14 69767182 69767185 4 + 2.854 2.870 0.053
ENSG00000100650 E013 1074.6038169 9.545851e-05 8.751704e-02 0.78266368 14 69767186 69767239 54 + 3.022 3.041 0.062
ENSG00000100650 E014 790.2029879 8.415113e-04 4.131804e-01 0.95812893 14 69767240 69767251 12 + 2.891 2.905 0.046
ENSG00000100650 E015 520.3636930 2.479453e-04 7.548387e-01 0.98848087 14 69767252 69767255 4 + 2.715 2.719 0.014
ENSG00000100650 E016 183.8936290 1.927007e-04 1.713550e-01 0.87486422 14 69767256 69767380 125 + 2.249 2.284 0.119
ENSG00000100650 E017 6.8797707 3.024858e-03 8.250768e-01   14 69767731 69767782 52 + 0.914 0.887 -0.103
ENSG00000100650 E018 6.7302690 3.093557e-03 7.117277e-01   14 69767783 69767797 15 + 0.914 0.869 -0.173
ENSG00000100650 E019 16.5850807 1.685085e-03 4.665357e-01 0.96303098 14 69767798 69767902 105 + 1.216 1.276 0.213
ENSG00000100650 E020 12.1999138 1.505220e-03 1.883067e-01 0.88647677 14 69767903 69767939 37 + 1.053 1.178 0.451
ENSG00000100650 E021 12.4129174 1.543481e-03 6.146272e-01 0.97943286 14 69767940 69767987 48 + 1.102 1.149 0.169
ENSG00000100650 E022 10.3441065 2.131687e-02 4.574680e-01 0.96227142 14 69767988 69768100 113 + 1.102 1.011 -0.333
ENSG00000100650 E023 12.3247892 2.843185e-03 2.804014e-02 0.59057687 14 69768101 69768137 37 + 1.225 1.011 -0.768
ENSG00000100650 E024 1940.7181329 7.940572e-05 3.123907e-01 0.93935478 14 69768138 69768282 145 + 3.285 3.292 0.026
ENSG00000100650 E025 12.5019015 1.622948e-02 1.042865e-01 0.80947519 14 69768283 69768603 321 + 1.216 1.036 -0.645
ENSG00000100650 E026 826.4858261 5.486710e-05 4.381093e-01 0.95977715 14 69768604 69768612 9 + 2.914 2.923 0.029
ENSG00000100650 E027 1233.1947319 4.251770e-05 4.308146e-01 0.95920724 14 69768613 69768674 62 + 3.088 3.095 0.023
ENSG00000100650 E028 21.0160958 1.230502e-02 3.487441e-01 0.94935483 14 69768675 69768797 123 + 1.386 1.303 -0.291
ENSG00000100650 E029 1427.0603899 3.709054e-05 3.048208e-01 0.93713007 14 69768798 69768896 99 + 3.150 3.159 0.029
ENSG00000100650 E030 156.8504219 1.503270e-04 3.824861e-04 0.08321675 14 69768897 69769122 226 + 2.249 2.146 -0.343
ENSG00000100650 E031 69.3597189 2.568062e-04 7.277753e-02 0.75315226 14 69769123 69769181 59 + 1.885 1.808 -0.260
ENSG00000100650 E032 1015.1047635 8.307964e-05 1.426203e-01 0.84973455 14 69769182 69769251 70 + 2.998 3.014 0.053
ENSG00000100650 E033 314.3355928 3.448897e-04 1.429301e-04 0.04516280 14 69769252 69769510 259 + 2.539 2.455 -0.280
ENSG00000100650 E034 639.8228287 5.201250e-04 9.074426e-05 0.03323851 14 69769511 69769745 235 + 2.841 2.771 -0.233
ENSG00000100650 E035 814.0712836 5.961964e-04 4.332560e-03 0.29120088 14 69769746 69770042 297 + 2.935 2.887 -0.163
ENSG00000100650 E036 474.4071905 7.506795e-04 5.666257e-04 0.10301354 14 69770043 69770447 405 + 2.712 2.639 -0.245
ENSG00000100650 E037 221.2777381 1.684564e-03 1.131912e-02 0.43336851 14 69770448 69770466 19 + 2.385 2.305 -0.269
ENSG00000100650 E038 1020.1225745 8.525054e-04 6.692275e-01 0.98394114 14 69770467 69770540 74 + 3.006 3.012 0.019
ENSG00000100650 E039 30.6698624 6.034443e-04 2.384545e-01 0.91230843 14 69770881 69770994 114 + 1.539 1.464 -0.257
ENSG00000100650 E040 1111.3366598 9.736318e-04 9.040652e-01 0.99664597 14 69770995 69771105 111 + 3.045 3.046 0.003
ENSG00000100650 E041 3.3050162 2.223587e-01 4.723828e-01   14 69771106 69771108 3 + 0.570 0.688 0.510
ENSG00000100650 E042 435.3534768 1.415986e-03 9.441971e-01 0.99842030 14 69771194 69771196 3 + 2.639 2.640 0.003
ENSG00000100650 E043 1610.8688278 2.843083e-04 6.493693e-01 0.98255844 14 69771197 69771544 348 + 3.205 3.209 0.013
ENSG00000100650 E044 570.8808226 2.327026e-03 4.341625e-01 0.95936914 14 69771545 69771916 372 + 2.769 2.746 -0.077
ENSG00000100650 E045 159.5223221 6.514169e-04 5.096289e-01 0.96934669 14 69771917 69772005 89 + 2.217 2.196 -0.070