ENSG00000089053

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261819 ENSG00000089053 HEK293_DMSO_2hB HEK293_TMG_2hB ANAPC5 protein_coding protein_coding 126.9802 125.4124 128.0611 3.942948 2.721173 0.03015063 60.694214 62.817320 57.681442 3.6618657 2.3743784 -0.1230347 0.48199167 0.50026667 0.45086667 -0.04940000 0.93117503 0.01408827 FALSE TRUE
ENST00000441917 ENSG00000089053 HEK293_DMSO_2hB HEK293_TMG_2hB ANAPC5 protein_coding protein_coding 126.9802 125.4124 128.0611 3.942948 2.721173 0.03015063 20.735972 3.485537 9.523131 2.1741644 1.2435388 1.4474361 0.15873750 0.02870000 0.07456667 0.04586667 0.93117503 0.01408827 FALSE TRUE
ENST00000541652 ENSG00000089053 HEK293_DMSO_2hB HEK293_TMG_2hB ANAPC5 protein_coding retained_intron 126.9802 125.4124 128.0611 3.942948 2.721173 0.03015063 4.090868 2.939283 10.838264 0.9733893 0.6022875 1.8790279 0.03081250 0.02373333 0.08453333 0.06080000 0.01408827 0.01408827 FALSE TRUE
ENST00000541887 ENSG00000089053 HEK293_DMSO_2hB HEK293_TMG_2hB ANAPC5 protein_coding protein_coding 126.9802 125.4124 128.0611 3.942948 2.721173 0.03015063 10.244355 35.910096 24.995725 3.8570538 1.5768644 -0.5225328 0.08313333 0.28536667 0.19530000 -0.09006667 0.39338777 0.01408827 FALSE TRUE
ENST00000544314 ENSG00000089053 HEK293_DMSO_2hB HEK293_TMG_2hB ANAPC5 protein_coding processed_transcript 126.9802 125.4124 128.0611 3.942948 2.721173 0.03015063 6.977114 0.563928 0.000000 0.3550254 0.0000000 -5.8427979 0.05295833 0.00450000 0.00000000 -0.00450000 0.61425578 0.01408827 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000089053 E001 3.3584246 5.777040e-03 0.5879178897   12 121308245 121308331 87 - 0.675 0.592 -0.363
ENSG00000089053 E002 3.8765801 5.745896e-03 0.4692567053   12 121308332 121308346 15 - 0.734 0.626 -0.452
ENSG00000089053 E003 4.1666056 4.480454e-03 0.7538747061   12 121308347 121308356 10 - 0.734 0.688 -0.189
ENSG00000089053 E004 5.5126998 3.432506e-03 0.7585188198   12 121308357 121308357 1 - 0.831 0.790 -0.161
ENSG00000089053 E005 6.1748830 3.795550e-03 0.9876118010   12 121308358 121308360 3 - 0.852 0.853 0.004
ENSG00000089053 E006 6.0028212 3.537202e-03 0.8861756370   12 121308361 121308365 5 - 0.852 0.833 -0.074
ENSG00000089053 E007 7.8687403 2.438753e-03 0.7600138393   12 121308366 121308366 1 - 0.961 0.925 -0.135
ENSG00000089053 E008 1087.3978238 5.261899e-05 0.3062768036 0.93720084 12 121308367 121308691 325 - 3.031 3.041 0.033
ENSG00000089053 E009 1200.3211265 3.117942e-04 0.7440768255 0.98807258 12 121309701 121309863 163 - 3.078 3.081 0.011
ENSG00000089053 E010 9.4544329 4.228861e-02 0.1575272780   12 121309864 121309969 106 - 1.111 0.906 -0.756
ENSG00000089053 E011 5.3609897 6.331935e-02 0.0424436631   12 121309970 121310011 42 - 0.946 0.591 -1.437
ENSG00000089053 E012 6.7201149 4.612683e-02 0.0166425141   12 121310012 121310394 383 - 1.037 0.658 -1.477
ENSG00000089053 E013 5.0115131 3.678539e-03 0.0526531055   12 121317890 121318154 265 - 0.892 0.626 -1.074
ENSG00000089053 E014 720.5780549 1.381189e-04 0.2763396683 0.92888563 12 121318277 121318351 75 - 2.851 2.865 0.047
ENSG00000089053 E015 602.7461754 6.197682e-05 0.1036965309 0.80932857 12 121318352 121318404 53 - 2.769 2.792 0.075
ENSG00000089053 E016 450.8672877 6.822663e-05 0.1343341967 0.84318255 12 121318405 121318424 20 - 2.642 2.666 0.080
ENSG00000089053 E017 37.0842282 2.201104e-03 0.7272999171 0.98757367 12 121318425 121318500 76 - 1.592 1.570 -0.075
ENSG00000089053 E018 583.6049825 6.227163e-05 0.3274180034 0.94347465 12 121318501 121318534 34 - 2.759 2.773 0.044
ENSG00000089053 E019 730.6662334 5.051953e-05 0.2591374443 0.92062005 12 121318535 121318608 74 - 2.856 2.870 0.045
ENSG00000089053 E020 537.0603059 7.264457e-04 0.4693093964 0.96327971 12 121319697 121319733 37 - 2.723 2.736 0.045
ENSG00000089053 E021 673.4461408 1.423900e-04 0.0259326317 0.57680103 12 121319734 121319818 85 - 2.812 2.843 0.104
ENSG00000089053 E022 543.9178683 3.805827e-04 0.1491716217 0.85479125 12 121320385 121320459 75 - 2.724 2.748 0.080
ENSG00000089053 E023 1.5041352 1.080952e-02 0.7968598282   12 121320460 121321111 652 - 0.373 0.417 0.249
ENSG00000089053 E024 0.6735358 2.024201e-02 0.2932549155   12 121326353 121326485 133 - 0.305 0.121 -1.658
ENSG00000089053 E025 1.5037149 1.043434e-02 0.6907247093   12 121326486 121326642 157 - 0.431 0.360 -0.395
ENSG00000089053 E026 862.7825657 1.428230e-04 0.0179948182 0.51064239 12 121327096 121327231 136 - 2.921 2.951 0.099
ENSG00000089053 E027 43.1730350 4.164838e-04 0.0025828823 0.22746537 12 121327232 121328256 1025 - 1.718 1.554 -0.557
ENSG00000089053 E028 7.2632748 2.696824e-03 0.1798934203   12 121328257 121328315 59 - 0.993 0.833 -0.605
ENSG00000089053 E029 1080.6195889 4.883983e-05 0.0232778128 0.55557387 12 121328316 121328458 143 - 3.022 3.045 0.078
ENSG00000089053 E030 519.6374620 5.235616e-05 0.7064167572 0.98603916 12 121328459 121328497 39 - 2.720 2.712 -0.024
ENSG00000089053 E031 11.8677354 7.970740e-03 0.6785363173 0.98443577 12 121328773 121328835 63 - 1.133 1.089 -0.157
ENSG00000089053 E032 10.0643052 7.460177e-03 0.7695015069 0.98990885 12 121328999 121329185 187 - 1.062 1.028 -0.122
ENSG00000089053 E033 850.2263725 7.717690e-05 0.3151724495 0.93973679 12 121330583 121330672 90 - 2.924 2.935 0.038
ENSG00000089053 E034 61.1190772 1.648592e-03 0.0011610433 0.15535588 12 121330673 121331226 554 - 1.867 1.707 -0.541
ENSG00000089053 E035 25.3200932 8.564618e-04 0.4166111894 0.95862334 12 121331227 121331346 120 - 1.394 1.449 0.189
ENSG00000089053 E036 827.3702236 4.197639e-05 0.0064027657 0.34579945 12 121331347 121331428 82 - 2.901 2.934 0.108
ENSG00000089053 E037 153.3372714 8.324981e-03 0.0015512150 0.17827553 12 121331429 121335172 3744 - 2.266 2.096 -0.569
ENSG00000089053 E038 8.3515636 2.610306e-03 0.6594099056   12 121335517 121335532 16 - 0.993 0.942 -0.189
ENSG00000089053 E039 1144.9742452 3.733669e-05 0.9108252396 0.99667547 12 121335533 121335723 191 - 3.060 3.058 -0.008
ENSG00000089053 E040 696.6007054 7.698117e-04 0.0546009236 0.70312027 12 121337291 121337392 102 - 2.861 2.825 -0.122
ENSG00000089053 E041 0.8632642 6.389538e-01 0.8173571292   12 121337393 121337395 3 - 0.307 0.219 -0.648
ENSG00000089053 E042 691.9965034 1.188266e-04 0.0004603667 0.09230633 12 121342003 121342069 67 - 2.866 2.814 -0.170
ENSG00000089053 E043 0.6892323 4.040919e-01 0.5410484983   12 121342070 121342071 2 - 0.127 0.298 1.532
ENSG00000089053 E044 436.0901039 1.985365e-04 0.0080259188 0.37872912 12 121345839 121345839 1 - 2.665 2.615 -0.165
ENSG00000089053 E045 881.5827516 1.002862e-04 0.0366120715 0.63370876 12 121345840 121345923 84 - 2.959 2.932 -0.092
ENSG00000089053 E046 666.1329675 4.959608e-05 0.2396456493 0.91317119 12 121345924 121345951 28 - 2.833 2.815 -0.059
ENSG00000089053 E047 715.4014486 4.860551e-05 0.2044503191 0.89715975 12 121345952 121345998 47 - 2.864 2.846 -0.061
ENSG00000089053 E048 615.8232049 4.929457e-05 0.1992619629 0.89394934 12 121345999 121346031 33 - 2.800 2.780 -0.066
ENSG00000089053 E049 26.6805264 1.026755e-02 0.0100973630 0.41492828 12 121346032 121346035 4 - 1.535 1.323 -0.734
ENSG00000089053 E050 39.5872216 3.286082e-03 0.0017794853 0.19158102 12 121346036 121346126 91 - 1.695 1.500 -0.664
ENSG00000089053 E051 67.1884839 1.371769e-02 0.0194053126 0.52295428 12 121346127 121346565 439 - 1.911 1.745 -0.561
ENSG00000089053 E052 57.4468213 2.277795e-03 0.0664705477 0.73799480 12 121346566 121346794 229 - 1.814 1.718 -0.325
ENSG00000089053 E053 37.4730104 4.841252e-04 0.0053292771 0.31900416 12 121346795 121346895 101 - 1.658 1.496 -0.553
ENSG00000089053 E054 754.0823200 5.197497e-05 0.1285760202 0.83802883 12 121346896 121347005 110 - 2.888 2.867 -0.071
ENSG00000089053 E055 2.7033897 6.699425e-03 0.9255881736   12 121347006 121347801 796 - 0.570 0.554 -0.073
ENSG00000089053 E056 554.2337683 2.488565e-04 0.1126735680 0.82101635 12 121347802 121347881 80 - 2.730 2.756 0.085
ENSG00000089053 E057 1.7111036 1.212936e-02 0.1831901389   12 121347882 121347883 2 - 0.529 0.294 -1.296
ENSG00000089053 E058 0.6749425 1.893165e-02 0.0332754523   12 121351060 121351203 144 - 0.373 0.000 -10.812
ENSG00000089053 E059 819.9434216 4.783094e-05 0.0134907628 0.46249862 12 121352134 121352446 313 - 2.899 2.929 0.099
ENSG00000089053 E060 2.3487486 7.863987e-03 0.6604618353   12 121399611 121399896 286 - 0.483 0.554 0.342