ENSG00000081307

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000356232 ENSG00000081307 HEK293_DMSO_2hB HEK293_TMG_2hB UBA5 protein_coding protein_coding 44.47096 72.21881 70.04642 2.272331 2.061076 -0.04405729 3.789564 6.731254 6.708266 0.4412197 0.2521366 -0.004928014 0.08277083 0.09380000 0.09576667 0.001966667 1.000000e+00 9.605411e-39 FALSE TRUE
ENST00000464101 ENSG00000081307 HEK293_DMSO_2hB HEK293_TMG_2hB UBA5 protein_coding retained_intron 44.47096 72.21881 70.04642 2.272331 2.061076 -0.04405729 10.935577 21.529975 20.595863 1.4715303 0.8362066 -0.063961743 0.23770833 0.29740000 0.29450000 -0.002900000 1.000000e+00 9.605411e-39 FALSE FALSE
ENST00000473651 ENSG00000081307 HEK293_DMSO_2hB HEK293_TMG_2hB UBA5 protein_coding protein_coding 44.47096 72.21881 70.04642 2.272331 2.061076 -0.04405729 2.566510 5.228217 4.559724 0.7826059 0.1317703 -0.196968624 0.05007500 0.07283333 0.06523333 -0.007600000 9.657890e-01 9.605411e-39 FALSE TRUE
MSTRG.23731.15 ENSG00000081307 HEK293_DMSO_2hB HEK293_TMG_2hB UBA5 protein_coding   44.47096 72.21881 70.04642 2.272331 2.061076 -0.04405729 20.185067 26.072937 31.002532 1.1989942 1.8468807 0.249744999 0.46564583 0.36070000 0.44186667 0.081166667 6.914225e-02 9.605411e-39 FALSE TRUE
MSTRG.23731.9 ENSG00000081307 HEK293_DMSO_2hB HEK293_TMG_2hB UBA5 protein_coding   44.47096 72.21881 70.04642 2.272331 2.061076 -0.04405729 1.822494 4.146041 0.000000 0.3938727 0.0000000 -8.699066080 0.05250833 0.05730000 0.00000000 -0.057300000 9.605411e-39 9.605411e-39 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_DMSO_2hB log2fold_HEK293_DMSO_2hB_HEK293_TMG_2hB
ENSG00000081307 E001 0.000000       3 132654446 132654670 225 +      
ENSG00000081307 E002 3.303612 1.063767e-02 4.556847e-01   3 132659885 132659891 7 + 0.572 0.685 0.492
ENSG00000081307 E003 3.454932 9.640908e-03 3.503853e-01   3 132659892 132659917 26 + 0.572 0.713 0.606
ENSG00000081307 E004 5.454091 5.205692e-03 3.035000e-01   3 132659918 132659945 28 + 0.736 0.870 0.528
ENSG00000081307 E005 20.231320 8.864125e-04 6.922354e-01 0.9855581 3 132659946 132660050 105 + 1.312 1.339 0.095
ENSG00000081307 E006 24.207429 1.599281e-03 5.637711e-01 0.9749512 3 132660051 132660184 134 + 1.420 1.376 -0.153
ENSG00000081307 E007 26.699655 3.694109e-03 4.474106e-01 0.9610416 3 132660185 132660304 120 + 1.468 1.410 -0.201
ENSG00000081307 E008 12.612771 3.948006e-02 7.812616e-01 0.9905048 3 132660305 132660309 5 + 1.114 1.144 0.109
ENSG00000081307 E009 15.226053 1.218395e-02 9.677783e-01 0.9989905 3 132660310 132660328 19 + 1.206 1.207 0.004
ENSG00000081307 E010 31.357926 2.492256e-03 1.664953e-01 0.8702791 3 132660329 132660342 14 + 1.463 1.552 0.304
ENSG00000081307 E011 65.830087 2.720166e-04 1.322408e-01 0.8417288 3 132660343 132660359 17 + 1.792 1.854 0.212
ENSG00000081307 E012 65.485271 2.697308e-04 2.249836e-01 0.9082743 3 132660360 132660361 2 + 1.796 1.846 0.169
ENSG00000081307 E013 159.754930 1.322967e-04 2.297942e-01 0.9103717 3 132660362 132660395 34 + 2.191 2.222 0.103
ENSG00000081307 E014 207.139408 1.219318e-04 1.018860e-01 0.8065449 3 132660396 132660426 31 + 2.299 2.336 0.125
ENSG00000081307 E015 234.123974 9.630346e-05 2.086766e-01 0.8997080 3 132660427 132660477 51 + 2.358 2.384 0.087
ENSG00000081307 E016 169.040685 1.242216e-04 7.108975e-01 0.9861786 3 132660478 132660488 11 + 2.228 2.235 0.024
ENSG00000081307 E017 289.393763 9.508396e-05 2.670437e-01 0.9246567 3 132660489 132660600 112 + 2.453 2.473 0.067
ENSG00000081307 E018 246.045448 9.222164e-05 1.564891e-02 0.4880899 3 132660601 132660696 96 + 2.366 2.417 0.172
ENSG00000081307 E019 117.303983 1.660333e-04 3.311278e-02 0.6166020 3 132660697 132660698 2 + 2.038 2.105 0.224
ENSG00000081307 E020 14.611896 1.266671e-03 7.867818e-01 0.9908091 3 132660699 132660898 200 + 1.205 1.179 -0.094
ENSG00000081307 E021 13.329282 1.458563e-03 7.994740e-01 0.9908091 3 132660899 132661048 150 + 1.167 1.140 -0.094
ENSG00000081307 E022 5.881854 8.583103e-02 5.592878e-01   3 132661216 132661257 42 + 0.893 0.785 -0.419
ENSG00000081307 E023 7.963295 2.349702e-03 2.942914e-01   3 132665739 132665822 84 + 1.010 0.888 -0.456
ENSG00000081307 E024 177.283868 1.180304e-04 2.159098e-02 0.5431126 3 132665823 132665868 46 + 2.221 2.279 0.195
ENSG00000081307 E025 249.906080 8.926005e-05 1.932091e-01 0.8892225 3 132665984 132666073 90 + 2.386 2.413 0.087
ENSG00000081307 E026 13.137183 3.191226e-03 4.252009e-03 0.2886811 3 132666074 132666151 78 + 1.274 0.998 -0.989
ENSG00000081307 E027 82.930173 3.149126e-03 7.845981e-05 0.0309895 3 132667258 132668817 1560 + 2.011 1.823 -0.633
ENSG00000081307 E028 343.096459 2.208149e-03 7.969151e-01 0.9908091 3 132668818 132668927 110 + 2.535 2.539 0.012
ENSG00000081307 E029 355.510920 8.810726e-05 4.930946e-01 0.9669437 3 132670198 132670284 87 + 2.560 2.544 -0.052
ENSG00000081307 E030 2.109560 7.784871e-03 8.680461e-01   3 132670682 132670964 283 + 0.485 0.511 0.127
ENSG00000081307 E031 402.410123 5.664013e-04 2.019576e-01 0.8957375 3 132670965 132671049 85 + 2.620 2.591 -0.096
ENSG00000081307 E032 384.535835 8.303491e-05 4.422256e-01 0.9603258 3 132671777 132671881 105 + 2.594 2.578 -0.056
ENSG00000081307 E033 3.375528 2.334422e-02 9.046681e-01   3 132671882 132672049 168 + 0.646 0.624 -0.092
ENSG00000081307 E034 360.155808 7.565599e-05 7.795968e-01 0.9905048 3 132672050 132672177 128 + 2.557 2.560 0.008
ENSG00000081307 E035 372.604695 7.073382e-05 5.644311e-01 0.9750273 3 132675248 132675381 134 + 2.580 2.566 -0.044
ENSG00000081307 E036 180.096185 1.222618e-04 6.510986e-01 0.9827701 3 132675382 132675383 2 + 2.266 2.252 -0.049
ENSG00000081307 E037 4.643126 5.675764e-03 8.254135e-01   3 132675384 132675604 221 + 0.736 0.764 0.113
ENSG00000081307 E038 305.846605 3.923736e-04 5.570834e-01 0.9741573 3 132675605 132675680 76 + 2.495 2.480 -0.051
ENSG00000081307 E039 292.759572 8.023665e-05 8.485305e-01 0.9941165 3 132675817 132675923 107 + 2.472 2.465 -0.023
ENSG00000081307 E040 484.020809 6.359163e-05 2.332421e-01 0.9112925 3 132676443 132676854 412 + 2.697 2.675 -0.072
ENSG00000081307 E041 656.663556 1.890238e-03 1.551890e-01 0.8599868 3 132676855 132679794 2940 + 2.836 2.799 -0.121