ENSG00000258857

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000556713 ENSG00000258857 HEK293_DMSO_6hA HEK293_OSMI2_6hA   lncRNA lncRNA 1.980113 1.262194 1.620209 0.1119296 0.3579947 0.3577379 0.0822502 0.1294353 0.0000000 0.026125 0.00000000 -3.8015242 0.04899167 0.1005667 0.0000000 -0.1005667 0.03426665 0.03426665   FALSE
MSTRG.9519.1 ENSG00000258857 HEK293_DMSO_6hA HEK293_OSMI2_6hA   lncRNA   1.980113 1.262194 1.620209 0.1119296 0.3579947 0.3577379 1.2483258 0.7536646 1.3574527 0.148504 0.32017299 0.8404799 0.67592917 0.6131000 0.8296667 0.2165667 0.61101002 0.03426665   FALSE
MSTRG.9519.2 ENSG00000258857 HEK293_DMSO_6hA HEK293_OSMI2_6hA   lncRNA   1.980113 1.262194 1.620209 0.1119296 0.3579947 0.3577379 0.6495366 0.3790944 0.2627563 0.190966 0.04818946 -0.5125078 0.27508333 0.2863333 0.1703333 -0.1160000 0.92210650 0.03426665   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000258857 E001 2.8288364 0.012671441 0.9398268937 0.98591617 14 50396259 50396375 117 + 0.587 0.580 -0.033
ENSG00000258857 E002 6.9681250 0.004039099 0.6711927910 0.90465400 14 50396376 50396458 83 + 0.927 0.884 -0.163
ENSG00000258857 E003 1.9561505 0.010663229 0.0006210938 0.04134996 14 50397013 50397353 341 + 0.109 0.686 3.748
ENSG00000258857 E004 12.1855321 0.002418442 0.9368282875 0.98511643 14 50397875 50398001 127 + 1.125 1.122 -0.008
ENSG00000258857 E005 0.6825723 0.019569105 0.2348400723   14 50398313 50398345 33 + 0.110 0.312 1.870
ENSG00000258857 E006 11.4011111 0.002758197 0.8033792479 0.94929743 14 50407774 50407895 122 + 1.085 1.111 0.092
ENSG00000258857 E007 6.8460617 0.014008564 0.3241876008 0.72020489 14 50413244 50413309 66 + 0.929 0.821 -0.417
ENSG00000258857 E008 9.5033656 0.015671729 0.4404577292 0.79804578 14 50418283 50418474 192 + 1.046 0.973 -0.268