ENSG00000256294

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262894 ENSG00000256294 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF225 protein_coding protein_coding 1.646841 0.8219146 0.7417988 0.1400237 0.06430628 -0.1460888 0.8638040 0.3571237 0.4122014 0.11775599 0.10247109 0.20166510 0.5807833 0.4163667 0.5415000 0.125133333 0.749577751 0.004343821 FALSE TRUE
ENST00000588926 ENSG00000256294 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF225 protein_coding protein_coding 1.646841 0.8219146 0.7417988 0.1400237 0.06430628 -0.1460888 0.1999773 0.1086351 0.1152308 0.02404434 0.06175362 0.07805871 0.1172458 0.1441333 0.1480000 0.003866667 0.984265534 0.004343821   FALSE
ENST00000589155 ENSG00000256294 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF225 protein_coding protein_coding 1.646841 0.8219146 0.7417988 0.1400237 0.06430628 -0.1460888 0.4011478 0.0000000 0.1650137 0.00000000 0.08551830 4.12939618 0.1741667 0.0000000 0.2429667 0.242966667 0.302424961 0.004343821 FALSE TRUE
ENST00000590612 ENSG00000256294 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF225 protein_coding protein_coding 1.646841 0.8219146 0.7417988 0.1400237 0.06430628 -0.1460888 0.1426522 0.2761000 0.0000000 0.06198455 0.00000000 -4.83844771 0.0907625 0.3438000 0.0000000 -0.343800000 0.004343821 0.004343821 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000256294 E001 0.0000000       19 44112181 44112297 117 +      
ENSG00000256294 E002 0.0000000       19 44113399 44113414 16 +      
ENSG00000256294 E003 0.0000000       19 44113415 44113416 2 +      
ENSG00000256294 E004 0.0000000       19 44113417 44113430 14 +      
ENSG00000256294 E005 2.3681101 0.009276839 0.270305547 0.67462539 19 44113431 44113569 139 + 0.634 0.464 -0.791
ENSG00000256294 E006 1.2287709 0.018222982 0.791582333   19 44114049 44114213 165 + 0.323 0.374 0.307
ENSG00000256294 E007 1.1409519 0.014264795 0.011720761   19 44115545 44115759 215 + 0.000 0.464 10.952
ENSG00000256294 E008 4.4896130 0.004942489 0.075102473 0.40716346 19 44115760 44115842 83 + 0.861 0.631 -0.934
ENSG00000256294 E009 5.6639539 0.004844565 0.102521975 0.46193737 19 44118188 44118314 127 + 0.923 0.729 -0.756
ENSG00000256294 E010 4.4261094 0.005376054 0.779578749 0.94220954 19 44118482 44118574 93 + 0.762 0.729 -0.130
ENSG00000256294 E011 0.9517497 0.149056719 0.276405135   19 44128454 44129041 588 + 0.397 0.188 -1.463
ENSG00000256294 E012 0.5080019 0.206280308 0.438901268   19 44129042 44129098 57 + 0.244 0.104 -1.476
ENSG00000256294 E013 3.0686356 0.173479749 0.999118605 0.99974641 19 44129099 44130700 1602 + 0.611 0.574 -0.165
ENSG00000256294 E014 4.8782248 0.005375993 0.379186656 0.75948326 19 44130850 44130968 119 + 0.838 0.729 -0.430
ENSG00000256294 E015 28.9416466 0.003993151 0.299059740 0.69920552 19 44130969 44132209 1241 + 1.499 1.447 -0.181
ENSG00000256294 E016 41.3393094 0.001323969 0.001014046 0.05453959 19 44132210 44134822 2613 + 1.532 1.680 0.504