ENSG00000224877

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000431388 ENSG00000224877 HEK293_DMSO_6hA HEK293_OSMI2_6hA NDUFAF8 protein_coding protein_coding 278.3759 254.8679 369.3783 31.19416 86.13776 0.5353315 241.97667 190.71584 352.380198 30.83997 83.799418 0.8856735 0.8142042 0.7415333 0.95026667 0.2087333 3.080659e-06 3.080659e-06   FALSE
ENST00000576002 ENSG00000224877 HEK293_DMSO_6hA HEK293_OSMI2_6hA NDUFAF8 protein_coding protein_coding 278.3759 254.8679 369.3783 31.19416 86.13776 0.5353315 26.11696 56.79947 7.238018 12.89517 4.331176 -2.9704730 0.1292917 0.2294000 0.02133333 -0.2080667 4.267715e-01 3.080659e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000224877 E001 2.382135 3.952775e-02 0.2340023815 0.63891352 17 81239191 81239267 77 + 0.394 0.633 1.158
ENSG00000224877 E002 2.881950 7.028151e-03 0.3461089796 0.73618685 17 81239268 81239304 37 + 0.505 0.673 0.754
ENSG00000224877 E003 5.246244 7.120949e-02 0.6554647477 0.89887841 17 81239305 81239310 6 + 0.753 0.830 0.307
ENSG00000224877 E004 7.786688 1.420686e-02 0.6999712483 0.91500682 17 81239311 81239317 7 + 0.954 0.925 -0.110
ENSG00000224877 E005 368.004916 1.962258e-02 0.0412337206 0.31807997 17 81239318 81239403 86 + 2.589 2.509 -0.267
ENSG00000224877 E006 590.850521 9.576116e-03 0.0172200465 0.22189088 17 81239404 81239447 44 + 2.785 2.730 -0.183
ENSG00000224877 E007 622.229413 8.228541e-03 0.0754485258 0.40796078 17 81239568 81239594 27 + 2.793 2.776 -0.059
ENSG00000224877 E008 654.388203 2.415202e-04 0.0006788603 0.04365280 17 81239595 81239610 16 + 2.818 2.800 -0.062
ENSG00000224877 E009 742.198705 8.944711e-05 0.0001172077 0.01473625 17 81239611 81239628 18 + 2.875 2.855 -0.068
ENSG00000224877 E010 1142.845937 6.627237e-05 0.0015064537 0.06844884 17 81239629 81239678 50 + 3.055 3.052 -0.008
ENSG00000224877 E011 18.222753 1.434228e-03 0.8096386087 0.95140672 17 81239679 81239682 4 + 1.263 1.309 0.161
ENSG00000224877 E012 15.996554 6.044164e-03 0.4924017576 0.82572305 17 81239683 81239771 89 + 1.181 1.272 0.322
ENSG00000224877 E013 34.525199 2.250633e-02 0.2003569868 0.60388432 17 81239772 81239913 142 + 1.465 1.628 0.556
ENSG00000224877 E014 21.302367 4.376178e-02 0.2083398202 0.61244313 17 81239914 81239943 30 + 1.240 1.447 0.719
ENSG00000224877 E015 49.475726 6.539372e-02 0.1065042564 0.46916564 17 81239944 81240229 286 + 1.575 1.826 0.848
ENSG00000224877 E016 42.531070 3.130170e-02 0.0702896529 0.39631263 17 81240230 81240439 210 + 1.528 1.750 0.756
ENSG00000224877 E017 2995.214531 5.705410e-03 0.0004790896 0.03512808 17 81240987 81241310 324 + 3.413 3.539 0.416