ENSG00000197380

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000300875 ENSG00000197380 HEK293_DMSO_6hA HEK293_OSMI2_6hA DACT3 protein_coding protein_coding 2.494591 3.320212 3.843227 0.3152145 0.2761884 0.210453 0.6050735 1.1928081 0.00000000 0.13051135 0.00000000 -6.9102627 0.27753750 0.37080000 0.00000000 -0.37080000 9.179482e-11 9.179482e-11 FALSE TRUE
ENST00000391916 ENSG00000197380 HEK293_DMSO_6hA HEK293_OSMI2_6hA DACT3 protein_coding protein_coding 2.494591 3.320212 3.843227 0.3152145 0.2761884 0.210453 1.7660738 1.8885164 3.74322874 0.41767331 0.29081979 0.9832597 0.64428750 0.55396667 0.97323333 0.41926667 1.681649e-09 9.179482e-11 FALSE TRUE
ENST00000410105 ENSG00000197380 HEK293_DMSO_6hA HEK293_OSMI2_6hA DACT3 protein_coding protein_coding 2.494591 3.320212 3.843227 0.3152145 0.2761884 0.210453 0.1234435 0.2388876 0.09999801 0.06096256 0.03265599 -1.1780168 0.07818333 0.07523333 0.02676667 -0.04846667 5.557242e-01 9.179482e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000197380 E001 1.682022 0.0117748696 0.644533274 0.8940887 19 46647551 46647611 61 - 0.398 0.479 0.424
ENSG00000197380 E002 230.579365 0.0002997198 0.798430390 0.9478197 19 46647612 46649872 2261 - 2.355 2.367 0.039
ENSG00000197380 E003 11.199288 0.1277700005 0.017592045 0.2240257 19 46650148 46652659 2512 - 0.817 1.247 1.582
ENSG00000197380 E004 39.922821 0.0007871588 0.656110171 0.8991848 19 46652660 46652812 153 - 1.595 1.626 0.105
ENSG00000197380 E005 34.788287 0.0016000607 0.640328553 0.8923957 19 46652979 46653075 97 - 1.560 1.543 -0.060
ENSG00000197380 E006 0.000000       19 46654643 46654657 15 -      
ENSG00000197380 E007 34.912337 0.0009575859 0.003084917 0.0999020 19 46660816 46661182 367 - 1.624 1.465 -0.544