ENSG00000196155

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000379344 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding protein_coding 17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 2.874611 0.7348656 3.389305 0.28707283 0.30500494 2.1901878 0.15928333 0.04216667 0.14803333 0.10586667 0.03483255 0.03483255 FALSE TRUE
ENST00000393966 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding nonsense_mediated_decay 17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 1.092275 1.9075596 1.718416 0.18223267 0.43552942 -0.1498212 0.07124167 0.10703333 0.07510000 -0.03193333 0.66102008 0.03483255 FALSE TRUE
ENST00000427155 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding protein_coding 17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 1.044356 1.6175921 2.302197 0.84979158 0.46117750 0.5065250 0.06102500 0.09020000 0.09960000 0.00940000 0.80180934 0.03483255 FALSE TRUE
ENST00000450733 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding protein_coding 17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 2.770452 3.0922403 3.955857 0.06781635 0.41066047 0.3543224 0.15944583 0.17446667 0.17356667 -0.00090000 1.00000000 0.03483255 FALSE TRUE
ENST00000562289 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding retained_intron 17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 1.762788 1.8254738 2.089512 0.30854926 0.31637916 0.1939016 0.09370417 0.10336667 0.09083333 -0.01253333 0.87345156 0.03483255 FALSE TRUE
ENST00000567136 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding nonsense_mediated_decay 17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 2.352327 2.8404919 3.174360 0.13829755 0.28977418 0.1597925 0.13396667 0.16010000 0.14020000 -0.01990000 0.75405513 0.03483255 TRUE FALSE
ENST00000569875 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding protein_coding 17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 1.592870 1.5815286 1.813926 0.42237605 0.30047485 0.1966339 0.07640833 0.08793333 0.08063333 -0.00730000 0.95082728 0.03483255 FALSE TRUE
MSTRG.12813.10 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding   17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 1.022112 0.3073010 1.172452 0.06671041 0.09448227 1.8978577 0.05475417 0.01723333 0.05166667 0.03443333 0.11065045 0.03483255 FALSE TRUE
MSTRG.12813.7 ENSG00000196155 HEK293_DMSO_6hA HEK293_OSMI2_6hA PLEKHG4 protein_coding   17.76363 17.75862 22.81776 0.4666205 0.9490913 0.3614571 1.417117 2.3060183 1.752255 0.39297527 0.26489742 -0.3942237 0.08124583 0.12896667 0.07696667 -0.05200000 0.36936245 0.03483255 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000196155 E001 0.0000000       16 67277407 67277483 77 +      
ENSG00000196155 E002 0.0000000       16 67277484 67277509 26 +      
ENSG00000196155 E003 0.0000000       16 67277510 67277819 310 +      
ENSG00000196155 E004 0.0000000       16 67277820 67277945 126 +      
ENSG00000196155 E005 0.0000000       16 67277946 67278156 211 +      
ENSG00000196155 E006 0.0000000       16 67278157 67278276 120 +      
ENSG00000196155 E007 0.1795728 0.0340143430 3.995566e-01   16 67278277 67278740 464 + 0.000 0.132 8.443
ENSG00000196155 E008 0.4387454 0.0244411696 4.433205e-01   16 67278741 67278790 50 + 0.108 0.233 1.335
ENSG00000196155 E009 1.6551457 0.0221085040 2.698532e-01 0.674262677 16 67278791 67278833 43 + 0.513 0.315 -1.081
ENSG00000196155 E010 5.7183183 0.0040093060 4.426210e-01 0.799060088 16 67278834 67278882 49 + 0.875 0.777 -0.383
ENSG00000196155 E011 0.9703733 0.1909469988 5.272960e-01   16 67278883 67279146 264 + 0.372 0.234 -0.926
ENSG00000196155 E012 3.4474838 0.0124273672 7.235649e-01 0.923389856 16 67279147 67279237 91 + 0.641 0.691 0.214
ENSG00000196155 E013 4.9284194 0.0057033797 7.673676e-01 0.938667765 16 67279238 67279276 39 + 0.763 0.802 0.153
ENSG00000196155 E014 2.4152904 0.0084217038 7.388961e-01 0.928687867 16 67279277 67279531 255 + 0.550 0.496 -0.255
ENSG00000196155 E015 9.1115085 0.0028920965 3.424869e-03 0.105285545 16 67279532 67279552 21 + 1.128 0.802 -1.221
ENSG00000196155 E016 15.0384393 0.0018666818 7.473987e-05 0.011468580 16 67279553 67279588 36 + 1.340 0.979 -1.293
ENSG00000196155 E017 16.6653712 0.0015639124 5.032432e-05 0.008995894 16 67279589 67279595 7 + 1.378 1.025 -1.253
ENSG00000196155 E018 20.4271026 0.0014069618 3.077269e-05 0.006454322 16 67279596 67279626 31 + 1.458 1.128 -1.153
ENSG00000196155 E019 15.6510412 0.0017727756 6.447705e-03 0.141740872 16 67279627 67279661 35 + 1.318 1.080 -0.843
ENSG00000196155 E020 20.4633845 0.0017023806 2.458917e-01 0.651526491 16 67279662 67279742 81 + 1.363 1.274 -0.311
ENSG00000196155 E021 22.2502842 0.0013179727 1.894375e-02 0.230659275 16 67279743 67279774 32 + 1.432 1.257 -0.608
ENSG00000196155 E022 21.1422558 0.0018454200 4.923829e-02 0.342266134 16 67279775 67279789 15 + 1.399 1.248 -0.525
ENSG00000196155 E023 36.8867468 0.0043151931 2.508198e-01 0.656636019 16 67279790 67279875 86 + 1.602 1.528 -0.251
ENSG00000196155 E024 49.8537723 0.0052169075 1.352570e-01 0.518256535 16 67279876 67279895 20 + 1.739 1.651 -0.299
ENSG00000196155 E025 162.8869051 0.0012144481 3.451949e-01 0.735741407 16 67279896 67280183 288 + 2.224 2.194 -0.100
ENSG00000196155 E026 123.0302883 0.0034557851 1.858733e-01 0.586626623 16 67280184 67280396 213 + 2.115 2.059 -0.187
ENSG00000196155 E027 82.5343587 0.0009490515 6.840273e-01 0.909666207 16 67280397 67280543 147 + 1.928 1.910 -0.058
ENSG00000196155 E028 46.4742718 0.0164483659 2.828218e-03 0.095510934 16 67280544 67280710 167 + 1.551 1.778 0.771
ENSG00000196155 E029 67.9345509 0.0045513578 3.229440e-01 0.719167908 16 67280711 67280806 96 + 1.864 1.812 -0.177
ENSG00000196155 E030 84.5105050 0.0012041865 8.694812e-01 0.967966545 16 67280882 67281005 124 + 1.929 1.937 0.024
ENSG00000196155 E031 89.5973415 0.0004429313 9.024819e-01 0.976897343 16 67281091 67281184 94 + 1.953 1.957 0.015
ENSG00000196155 E032 75.5488224 0.0046661872 9.226843e-01 0.981523155 16 67281567 67281644 78 + 1.882 1.888 0.020
ENSG00000196155 E033 96.9987180 0.0003565410 8.891931e-01 0.973494451 16 67281724 67281837 114 + 1.995 1.990 -0.018
ENSG00000196155 E034 106.8684269 0.0002982680 6.063201e-01 0.878223188 16 67282009 67282107 99 + 2.041 2.023 -0.060
ENSG00000196155 E035 6.6963207 0.0082813665 1.092258e-01 0.474303767 16 67282181 67282200 20 + 0.784 0.979 0.747
ENSG00000196155 E036 141.3820927 0.0014634640 4.072102e-01 0.778387288 16 67282201 67282349 149 + 2.165 2.136 -0.098
ENSG00000196155 E037 137.6194946 0.0025184515 3.537935e-01 0.742383431 16 67282503 67282641 139 + 2.158 2.120 -0.127
ENSG00000196155 E038 99.4698487 0.0002930556 3.276231e-01 0.722838257 16 67282742 67282858 117 + 2.015 1.980 -0.116
ENSG00000196155 E039 129.6270291 0.0010178902 7.007096e-01 0.915354400 16 67284275 67284457 183 + 2.122 2.110 -0.041
ENSG00000196155 E040 209.4932337 0.0004869543 6.075066e-01 0.878623379 16 67284713 67285215 503 + 2.318 2.331 0.044
ENSG00000196155 E041 153.2692138 0.0002181605 6.128767e-01 0.881284114 16 67285290 67285536 247 + 2.181 2.195 0.047
ENSG00000196155 E042 114.6280813 0.0002843009 2.404485e-01 0.645559744 16 67286274 67286363 90 + 2.042 2.080 0.128
ENSG00000196155 E043 164.0088989 0.0002170147 2.923798e-01 0.693291176 16 67286445 67286666 222 + 2.202 2.230 0.095
ENSG00000196155 E044 129.9568926 0.0002898066 5.275963e-01 0.843747831 16 67286749 67286919 171 + 2.106 2.125 0.064
ENSG00000196155 E045 169.8953330 0.0001846054 3.248449e-01 0.720776087 16 67287000 67287177 178 + 2.219 2.245 0.087
ENSG00000196155 E046 25.8597077 0.0011321295 8.271819e-02 0.423428656 16 67287572 67287897 326 + 1.368 1.485 0.404
ENSG00000196155 E047 78.2100843 0.0014849364 1.958952e-01 0.598955745 16 67287898 67287899 2 + 1.874 1.929 0.185
ENSG00000196155 E048 119.8839473 0.0002472107 1.583177e-01 0.551693225 16 67287900 67287949 50 + 2.060 2.105 0.150
ENSG00000196155 E049 133.9571151 0.0002442939 3.199786e-01 0.716570200 16 67287950 67288014 65 + 2.114 2.144 0.100
ENSG00000196155 E050 211.2706076 0.0004372839 2.964184e-01 0.696849715 16 67288167 67288400 234 + 2.312 2.339 0.090
ENSG00000196155 E051 29.3954448 0.0015075874 4.535483e-03 0.120792402 16 67288476 67288488 13 + 1.379 1.563 0.634
ENSG00000196155 E052 135.2026450 0.0003984471 4.225241e-01 0.787588244 16 67288489 67288604 116 + 2.119 2.144 0.084
ENSG00000196155 E053 394.0953422 0.0003477742 2.443838e-01 0.649775164 16 67288803 67289499 697 + 2.584 2.606 0.073