ENSG00000186184

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302979 ENSG00000186184 HEK293_DMSO_6hA HEK293_OSMI2_6hA POLR1D protein_coding protein_coding 151.4816 115.8138 112.1059 9.781786 18.48111 -0.04693987 97.33337 79.66487 61.33556 9.1894731 11.073375 -0.3771658 0.63350833 0.6847667 0.5436333 -0.141133333 0.0007056126 0.0007056126 FALSE TRUE
ENST00000399697 ENSG00000186184 HEK293_DMSO_6hA HEK293_OSMI2_6hA POLR1D protein_coding protein_coding 151.4816 115.8138 112.1059 9.781786 18.48111 -0.04693987 25.66855 13.64826 28.89481 1.7886783 8.079230 1.0815360 0.17154167 0.1184333 0.2454000 0.126966667 0.0221290583 0.0007056126 FALSE FALSE
MSTRG.8526.6 ENSG00000186184 HEK293_DMSO_6hA HEK293_OSMI2_6hA POLR1D protein_coding   151.4816 115.8138 112.1059 9.781786 18.48111 -0.04693987 14.45682 12.58131 11.62423 0.2489222 1.029211 -0.1140520 0.09784167 0.1099333 0.1070000 -0.002933333 0.9593052589 0.0007056126 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000186184 E001 0.9762989 0.0145267763 9.452541e-01   13 27620742 27620742 1 + 0.288 0.293 0.036
ENSG00000186184 E002 4.1358333 0.0051665003 5.940983e-01 8.731641e-01 13 27620743 27620757 15 + 0.677 0.740 0.261
ENSG00000186184 E003 6.8150604 0.0036013405 6.293490e-01 8.876918e-01 13 27620758 27620764 7 + 0.861 0.907 0.175
ENSG00000186184 E004 14.3537767 0.0019734925 5.527042e-02 3.591097e-01 13 27620765 27620771 7 + 1.089 1.248 0.567
ENSG00000186184 E005 70.2501964 0.0353634712 4.166958e-01 7.845804e-01 13 27620772 27620918 147 + 1.814 1.881 0.227
ENSG00000186184 E006 60.4824537 0.0194866751 2.794818e-01 6.829134e-01 13 27620919 27620946 28 + 1.743 1.822 0.265
ENSG00000186184 E007 56.6858947 0.0273567963 4.086568e-01 7.794763e-01 13 27620947 27620962 16 + 1.724 1.789 0.217
ENSG00000186184 E008 71.9479373 0.0295684880 1.822082e-01 5.821146e-01 13 27620963 27620994 32 + 1.799 1.911 0.375
ENSG00000186184 E009 135.4842317 0.0220032700 5.220959e-01 8.410722e-01 13 27620995 27621104 110 + 2.113 2.152 0.131
ENSG00000186184 E010 78.3647814 0.0137648822 5.448359e-01 8.520128e-01 13 27621105 27621140 36 + 1.878 1.913 0.116
ENSG00000186184 E011 23.4404397 0.1289176155 4.972495e-01 8.279781e-01 13 27621141 27621192 52 + 1.345 1.421 0.266
ENSG00000186184 E012 26.5502460 0.0983002216 3.914858e-01 7.679607e-01 13 27621193 27621263 71 + 1.384 1.481 0.334
ENSG00000186184 E013 27.1433700 0.1268489544 1.851272e-01 5.857117e-01 13 27621264 27621336 73 + 1.332 1.529 0.679
ENSG00000186184 E014 134.7556949 0.0055674752 6.568626e-01 8.994443e-01 13 27621337 27621437 101 + 2.126 2.136 0.036
ENSG00000186184 E015 167.3029824 0.0095687056 6.914885e-01 9.122620e-01 13 27621438 27621778 341 + 2.217 2.231 0.046
ENSG00000186184 E016 53.2047718 0.0173200253 6.413643e-01 8.929878e-01 13 27621779 27621869 91 + 1.753 1.710 -0.146
ENSG00000186184 E017 92.4892119 0.0053694297 4.586873e-01 8.078293e-01 13 27621870 27621872 3 + 1.993 1.946 -0.157
ENSG00000186184 E018 98.7283538 0.0030226911 6.417064e-01 8.931667e-01 13 27621873 27621874 2 + 2.013 1.982 -0.105
ENSG00000186184 E019 238.7030443 0.0001684321 6.023803e-01 8.766661e-01 13 27621875 27621891 17 + 2.382 2.379 -0.007
ENSG00000186184 E020 257.9279260 0.0002249565 8.546666e-01 9.641204e-01 13 27621892 27621892 1 + 2.420 2.410 -0.033
ENSG00000186184 E021 323.3262806 0.0005021727 9.127861e-01 9.791425e-01 13 27621893 27621904 12 + 2.519 2.507 -0.039
ENSG00000186184 E022 515.0262670 0.0001337471 3.552957e-02 2.988863e-01 13 27621905 27621946 42 + 2.703 2.722 0.063
ENSG00000186184 E023 488.3981801 0.0001056761 4.203809e-03 1.162692e-01 13 27621947 27621953 7 + 2.673 2.704 0.105
ENSG00000186184 E024 571.9019161 0.0002069328 5.586772e-03 1.331481e-01 13 27621954 27621983 30 + 2.741 2.770 0.098
ENSG00000186184 E025 556.1117907 0.0011185948 3.186552e-02 2.867446e-01 13 27621984 27622009 26 + 2.725 2.761 0.117
ENSG00000186184 E026 4.6389812 0.0058263059 2.912938e-01 6.926051e-01 13 27622159 27622222 64 + 0.677 0.810 0.539
ENSG00000186184 E027 3.4484944 0.0857170360 4.780057e-01 8.182220e-01 13 27622354 27622390 37 + 0.581 0.711 0.556
ENSG00000186184 E028 6.9364532 0.0036676037 1.673031e-01 5.636128e-01 13 27622841 27622874 34 + 0.801 0.956 0.592
ENSG00000186184 E029 1366.4328212 0.0056345007 2.829217e-08 3.606359e-05 13 27622875 27623452 578 + 3.035 3.210 0.584
ENSG00000186184 E030 0.5780114 0.0194559380 3.450900e-01   13 27646194 27646218 25 + 0.287 0.121 -1.544
ENSG00000186184 E031 1.4980093 0.0744840317 9.232783e-01   13 27648010 27648378 369 + 0.407 0.415 0.044
ENSG00000186184 E032 177.1644982 0.0011540290 2.365479e-09 5.850466e-06 13 27648379 27648386 8 + 2.345 2.146 -0.664
ENSG00000186184 E033 246.6596606 0.0095317343 7.195771e-04 4.510193e-02 13 27648387 27648449 63 + 2.485 2.292 -0.643
ENSG00000186184 E034 114.1823805 0.0294969636 3.595174e-02 3.005058e-01 13 27648450 27648453 4 + 2.158 1.953 -0.688
ENSG00000186184 E035 1.6287567 0.0109135210 1.771261e-01   13 27648454 27649606 1153 + 0.288 0.511 1.258
ENSG00000186184 E036 9.1710382 0.0027409040 6.613040e-01 9.009312e-01 13 27650042 27650241 200 + 1.030 0.971 -0.217
ENSG00000186184 E037 17.4426380 0.0102021895 6.624902e-01 9.013581e-01 13 27650242 27651352 1111 + 1.290 1.239 -0.179
ENSG00000186184 E038 1.9822978 0.0283634605 5.326454e-01 8.464635e-01 13 27651353 27651549 197 + 0.410 0.511 0.514
ENSG00000186184 E039 1.5351238 0.1121230715 7.404807e-01   13 27660779 27660811 33 + 0.359 0.415 0.311
ENSG00000186184 E040 1.7976616 0.0333730533 7.255275e-01 9.241861e-01 13 27660868 27660915 48 + 0.411 0.466 0.288
ENSG00000186184 E041 8.4344984 0.0170087127 1.017376e-01 4.606081e-01 13 27664627 27665112 486 + 0.863 1.051 0.706
ENSG00000186184 E042 2.5581091 0.0081608630 2.700310e-01 6.743666e-01 13 27665113 27665144 32 + 0.459 0.624 0.775
ENSG00000186184 E043 9.4840262 0.0399752381 1.627813e-02 2.163364e-01 13 27665145 27665685 541 + 0.821 1.150 1.220
ENSG00000186184 E044 394.5887547 0.0130864622 5.413737e-04 3.780468e-02 13 27665686 27665949 264 + 2.698 2.480 -0.725
ENSG00000186184 E045 577.0274270 0.0170074986 6.678448e-04 4.326345e-02 13 27665950 27666856 907 + 2.870 2.632 -0.789
ENSG00000186184 E046 374.5985596 0.0160746475 3.578111e-04 2.933821e-02 13 27666857 27667415 559 + 2.686 2.440 -0.821
ENSG00000186184 E047 2.2448383 0.0095635224 2.500770e-01 6.558106e-01 13 27738707 27738767 61 + 0.615 0.416 -0.959
ENSG00000186184 E048 2.5085411 0.0226586461 9.472287e-01 9.876318e-01 13 27739917 27744237 4321 + 0.578 0.553 -0.116