ENSG00000185163

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000329073 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding retained_intron 38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 2.393240 3.23189909 2.704323 0.19950022 0.5874744 -0.25624673 0.06590417 0.08836667 0.05973333 -0.02863333 0.539536851 0.001042456 FALSE FALSE
ENST00000397333 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding protein_coding 38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 11.135564 10.76908710 12.937336 0.19971459 0.9530083 0.26442031 0.29019167 0.29370000 0.28063333 -0.01306667 0.857353223 0.001042456 FALSE TRUE
ENST00000462829 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding retained_intron 38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 1.717806 3.14434561 1.379505 0.44508731 0.8076941 -1.18276998 0.05045000 0.08590000 0.03150000 -0.05440000 0.255229105 0.001042456   FALSE
ENST00000541489 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding retained_intron 38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 10.466539 8.43088677 12.848633 1.46378857 1.2312806 0.60727080 0.25332917 0.22820000 0.27536667 0.04716667 0.675762649 0.001042456 FALSE TRUE
ENST00000545991 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding retained_intron 38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 2.777054 4.34052081 3.031368 0.21676076 0.8756289 -0.51646796 0.07935000 0.11866667 0.06666667 -0.05200000 0.317899719 0.001042456 FALSE FALSE
ENST00000546058 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding retained_intron 38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 3.249531 3.81448284 3.635847 0.48308874 0.5497318 -0.06901055 0.08877500 0.10443333 0.07860000 -0.02583333 0.710476136 0.001042456 TRUE FALSE
MSTRG.8340.4 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding   38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 2.179195 1.90537582 2.582942 0.19464049 1.6762561 0.43696235 0.05478750 0.05196667 0.05076667 -0.00120000 0.798478965 0.001042456 TRUE TRUE
MSTRG.8340.5 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding   38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 2.193869 0.03866133 3.544742 0.03866133 1.4956801 6.19082533 0.04747500 0.00100000 0.07320000 0.07220000 0.001042456 0.001042456 TRUE TRUE
MSTRG.8340.7 ENSG00000185163 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDX51 protein_coding   38.98385 36.69727 46.62959 0.9152756 3.992598 0.345489 2.834370 0.83293939 3.943649 0.41740276 0.9304511 2.22968403 0.06871250 0.02250000 0.08300000 0.06050000 0.384592043 0.001042456 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000185163 E001 0.0000000       12 132135329 132135424 96 -      
ENSG00000185163 E002 0.0000000       12 132135566 132135635 70 -      
ENSG00000185163 E003 0.0000000       12 132135949 132135983 35 -      
ENSG00000185163 E004 0.0000000       12 132136232 132136369 138 -      
ENSG00000185163 E005 0.5029995 0.0219621291 4.346424e-01   12 132136370 132136593 224 - 0.106 0.238 1.410
ENSG00000185163 E006 32.0793901 0.0187825563 3.389648e-02 0.293620052 12 132136594 132136691 98 - 1.407 1.615 0.711
ENSG00000185163 E007 260.1755023 0.0028377620 9.682014e-05 0.013291768 12 132136692 132137320 629 - 2.341 2.489 0.493
ENSG00000185163 E008 121.9459645 0.0014926692 1.391749e-05 0.003863071 12 132137321 132137434 114 - 2.000 2.174 0.581
ENSG00000185163 E009 508.5628884 0.0030723104 2.569583e-04 0.023961904 12 132137435 132138400 966 - 2.641 2.770 0.427
ENSG00000185163 E010 118.2015898 0.0002677737 4.868433e-01 0.822919099 12 132138401 132138579 179 - 2.055 2.093 0.127
ENSG00000185163 E011 98.0259124 0.0011894302 5.949385e-01 0.873558269 12 132138580 132138707 128 - 1.975 2.012 0.124
ENSG00000185163 E012 192.4818377 0.0001782705 3.087935e-02 0.282792381 12 132138708 132138872 165 - 2.253 2.323 0.232
ENSG00000185163 E013 447.6021133 0.0000950857 1.286797e-01 0.508157747 12 132138873 132139112 240 - 2.633 2.673 0.133
ENSG00000185163 E014 460.6596283 0.0006022759 1.271607e-01 0.506000477 12 132139113 132139298 186 - 2.669 2.657 -0.041
ENSG00000185163 E015 309.6003464 0.0025104432 1.493889e-01 0.538610670 12 132139635 132139769 135 - 2.501 2.478 -0.077
ENSG00000185163 E016 9.7003042 0.0517765044 6.062283e-01 0.878214239 12 132139770 132139854 85 - 0.976 1.065 0.326
ENSG00000185163 E017 6.3778806 0.1796933616 9.988231e-01 0.999648261 12 132139855 132139860 6 - 0.858 0.857 -0.006
ENSG00000185163 E018 281.5622560 0.0004731788 1.809171e-02 0.226404012 12 132139861 132139924 64 - 2.468 2.430 -0.126
ENSG00000185163 E019 6.5343468 0.0697674275 4.792218e-01 0.818805367 12 132139925 132139973 49 - 0.803 0.937 0.514
ENSG00000185163 E020 332.8063339 0.0001638781 5.916763e-03 0.136840326 12 132140098 132140199 102 - 2.541 2.502 -0.128
ENSG00000185163 E021 1.7749040 0.0102942471 4.766915e-01 0.817572651 12 132140422 132140422 1 - 0.504 0.391 -0.583
ENSG00000185163 E022 364.2661672 0.0001277511 2.595497e-02 0.262035213 12 132140423 132140539 117 - 2.574 2.548 -0.086
ENSG00000185163 E023 314.5113928 0.0001371523 1.420385e-02 0.204049011 12 132140620 132140735 116 - 2.514 2.481 -0.113
ENSG00000185163 E024 4.5602391 0.0900208365 2.568367e-01 0.662643552 12 132140736 132140821 86 - 0.614 0.838 0.919
ENSG00000185163 E025 348.3929882 0.0031545587 4.555222e-02 0.331663843 12 132140831 132141005 175 - 2.565 2.517 -0.160
ENSG00000185163 E026 145.3675288 0.0038536066 1.838209e-02 0.228084394 12 132141006 132141020 15 - 2.206 2.120 -0.286
ENSG00000185163 E027 36.3734891 0.0446729750 1.557304e-02 0.212481950 12 132141021 132141102 82 - 1.412 1.700 0.985
ENSG00000185163 E028 77.6004258 0.0393876770 6.032475e-02 0.372386929 12 132141103 132141274 172 - 1.792 1.988 0.659
ENSG00000185163 E029 304.1878249 0.0009794696 6.527618e-04 0.042599983 12 132141275 132141420 146 - 2.516 2.448 -0.226
ENSG00000185163 E030 268.5609914 0.0001730843 2.312924e-01 0.636368948 12 132141498 132141606 109 - 2.436 2.425 -0.036
ENSG00000185163 E031 114.2096038 0.0668810686 1.927260e-02 0.231934983 12 132141607 132141849 243 - 1.905 2.191 0.961
ENSG00000185163 E032 265.2489183 0.0001419932 8.052081e-01 0.949727875 12 132141850 132141956 107 - 2.421 2.431 0.032
ENSG00000185163 E033 87.6222688 0.0162591772 3.510294e-02 0.297665439 12 132141957 132142118 162 - 1.856 2.029 0.581
ENSG00000185163 E034 227.5026490 0.0001922536 6.439699e-03 0.141625220 12 132142119 132142190 72 - 2.380 2.331 -0.164
ENSG00000185163 E035 191.3064828 0.0008412003 1.202962e-02 0.189600112 12 132142277 132142317 41 - 2.308 2.253 -0.186
ENSG00000185163 E036 250.2443536 0.0012590207 3.446580e-02 0.295642357 12 132142318 132142422 105 - 2.419 2.377 -0.138
ENSG00000185163 E037 261.4827613 0.0001819481 9.017457e-02 0.438937167 12 132142728 132142878 151 - 2.431 2.408 -0.075
ENSG00000185163 E038 103.9658864 0.1414767833 2.374715e-02 0.253453362 12 132142879 132143639 761 - 1.828 2.171 1.151
ENSG00000185163 E039 136.6175500 0.0122239150 1.057430e-02 0.177911732 12 132143695 132143909 215 - 2.198 2.058 -0.466
ENSG00000185163 E040 68.3573016 0.0015898430 1.333695e-03 0.063800599 12 132143993 132144319 327 - 1.900 1.767 -0.450