ENSG00000182544

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000329548 ENSG00000182544 HEK293_DMSO_6hA HEK293_OSMI2_6hA MFSD5 protein_coding protein_coding 40.58526 41.15921 52.04709 5.758254 3.328337 0.3385289 31.270342 25.48958 46.811395 2.610833 3.6770237 0.8766942 0.7629875 0.6275667 0.89803333 0.2704667 2.655971e-10 2.655971e-10 FALSE TRUE
ENST00000552097 ENSG00000182544 HEK293_DMSO_6hA HEK293_OSMI2_6hA MFSD5 protein_coding processed_transcript 40.58526 41.15921 52.04709 5.758254 3.328337 0.3385289 6.633802 13.43108 1.798328 3.298494 0.9119444 -2.8939208 0.1670000 0.3148000 0.03626667 -0.2785333 3.553713e-01 2.655971e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000182544 E001 1.572307 0.0119445536 1.266887e-01   12 53251065 53251250 186 + 0.272 0.534 1.474
ENSG00000182544 E002 21.643579 0.0191292123 4.424347e-01 0.7989637500 12 53251251 53251585 335 + 1.315 1.384 0.241
ENSG00000182544 E003 11.600946 0.0032881794 4.955563e-01 0.8271655980 12 53251586 53251624 39 + 1.121 1.056 -0.237
ENSG00000182544 E004 1.673557 0.0904172705 6.160277e-01 0.8825681561 12 53251625 53251652 28 + 0.479 0.378 -0.536
ENSG00000182544 E005 2.673240 0.0072932276 3.893006e-01 0.7665309565 12 53251653 53251704 52 + 0.623 0.488 -0.624
ENSG00000182544 E006 23.455298 0.0015359259 9.287028e-01 0.9830180061 12 53251705 53252035 331 + 1.375 1.383 0.029
ENSG00000182544 E007 3.267994 0.0066594871 4.206780e-01 0.7865120408 12 53252095 53252104 10 + 0.558 0.681 0.538
ENSG00000182544 E008 157.681667 0.0028774202 3.738357e-05 0.0072794285 12 53252105 53252268 164 + 2.270 2.116 -0.512
ENSG00000182544 E009 5.965311 0.0040717801 1.934426e-01 0.5956758315 12 53252449 53252483 35 + 0.752 0.917 0.644
ENSG00000182544 E010 163.406430 0.0002333525 1.029769e-04 0.0138059912 12 53252818 53252866 49 + 2.261 2.158 -0.346
ENSG00000182544 E011 2092.647968 0.0002947938 1.102990e-08 0.0000180541 12 53252867 53254406 1540 + 3.306 3.330 0.079