ENSG00000169679

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302759 ENSG00000169679 HEK293_DMSO_6hA HEK293_OSMI2_6hA BUB1 protein_coding protein_coding 42.37914 17.47781 16.42838 5.594248 2.778802 -0.08928126 19.427676 4.516108 7.4480606 1.478282 1.7964886 0.7205294 0.44572917 0.25720000 0.4404667 0.18326667 0.01064242 0.01064242 FALSE TRUE
ENST00000466333 ENSG00000169679 HEK293_DMSO_6hA HEK293_OSMI2_6hA BUB1 protein_coding retained_intron 42.37914 17.47781 16.42838 5.594248 2.778802 -0.08928126 8.407610 2.619973 0.7813465 1.559022 0.7813465 -1.7326666 0.14385417 0.12883333 0.0713000 -0.05753333 0.71927023 0.01064242 FALSE TRUE
ENST00000478175 ENSG00000169679 HEK293_DMSO_6hA HEK293_OSMI2_6hA BUB1 protein_coding processed_transcript 42.37914 17.47781 16.42838 5.594248 2.778802 -0.08928126 2.973629 4.332540 1.9841315 1.494647 0.3270302 -1.1227786 0.11039583 0.24423333 0.1380667 -0.10616667 0.33239545 0.01064242 FALSE TRUE
ENST00000535254 ENSG00000169679 HEK293_DMSO_6hA HEK293_OSMI2_6hA BUB1 protein_coding protein_coding 42.37914 17.47781 16.42838 5.594248 2.778802 -0.08928126 6.359861 1.141150 3.1076219 1.141150 1.5823272 1.4373704 0.13144167 0.03983333 0.1616667 0.12183333 0.70262360 0.01064242 FALSE TRUE
ENST00000671097 ENSG00000169679 HEK293_DMSO_6hA HEK293_OSMI2_6hA BUB1 protein_coding nonsense_mediated_decay 42.37914 17.47781 16.42838 5.594248 2.778802 -0.08928126 2.351487 2.494369 1.5164490 0.671760 0.4107663 -0.7142679 0.09022083 0.16496667 0.0927000 -0.07226667 0.72784846 0.01064242 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000169679 E001 11.4766058 0.0021893816 6.744499e-02 0.390041251 2 110637528 110637697 170 - 1.002 1.185 0.661
ENSG00000169679 E002 14.5359717 0.0019318782 4.945822e-01 0.826590188 2 110637698 110637831 134 - 1.155 1.221 0.235
ENSG00000169679 E003 5.9106188 0.0064305499 4.807019e-01 0.819603801 2 110637832 110637836 5 - 0.788 0.884 0.373
ENSG00000169679 E004 159.7538624 0.0096709147 5.620447e-01 0.858933221 2 110637837 110638159 323 - 2.198 2.215 0.059
ENSG00000169679 E005 156.0149049 0.0104928920 3.821783e-01 0.761621243 2 110639742 110639848 107 - 2.174 2.218 0.146
ENSG00000169679 E006 178.2545402 0.0072343728 5.197042e-01 0.839987633 2 110641034 110641192 159 - 2.239 2.271 0.108
ENSG00000169679 E007 79.2514973 0.0015733838 7.851883e-01 0.943723518 2 110641193 110641205 13 - 1.897 1.916 0.062
ENSG00000169679 E008 160.1863907 0.0002296768 8.803035e-01 0.971251130 2 110641307 110641464 158 - 2.206 2.217 0.034
ENSG00000169679 E009 138.3560608 0.0004279401 4.868457e-01 0.822919099 2 110641642 110641803 162 - 2.159 2.144 -0.049
ENSG00000169679 E010 1.6757032 0.0125442660 1.714407e-01 0.568828511 2 110641804 110641844 41 - 0.280 0.522 1.360
ENSG00000169679 E011 0.3607829 0.0414298321 9.417911e-01   2 110642114 110642118 5 - 0.115 0.125 0.140
ENSG00000169679 E012 96.2853777 0.0003631732 6.123771e-01 0.881078058 2 110642119 110642234 116 - 2.003 1.990 -0.043
ENSG00000169679 E013 20.4257259 0.1263579410 3.359807e-02 0.292791769 2 110642449 110642790 342 - 1.076 1.488 1.447
ENSG00000169679 E014 3.1932229 0.0074037521 1.907808e-01 0.592513172 2 110648817 110649233 417 - 0.718 0.522 -0.856
ENSG00000169679 E015 118.8140914 0.0002727756 6.707528e-01 0.904465095 2 110649234 110649377 144 - 2.075 2.094 0.063
ENSG00000169679 E016 157.1649571 0.0030079555 4.667980e-01 0.812201588 2 110650546 110650784 239 - 2.215 2.191 -0.080
ENSG00000169679 E017 101.3129905 0.0046048212 3.234264e-01 0.719533888 2 110653436 110653523 88 - 2.037 1.992 -0.153
ENSG00000169679 E018 129.6204421 0.0002767761 9.210693e-01 0.981100735 2 110655739 110655916 178 - 2.118 2.126 0.028
ENSG00000169679 E019 87.0546771 0.0032130767 5.850525e-01 0.869079971 2 110657036 110657117 82 - 1.956 1.937 -0.063
ENSG00000169679 E020 93.9776703 0.0016803247 1.868651e-01 0.587921334 2 110657546 110657645 100 - 2.004 1.957 -0.157
ENSG00000169679 E021 101.1485639 0.0008213875 1.972925e-01 0.600539080 2 110658410 110658520 111 - 2.032 1.991 -0.135
ENSG00000169679 E022 98.9802969 0.0003092236 6.443050e-01 0.894074441 2 110658614 110658687 74 - 2.012 2.002 -0.034
ENSG00000169679 E023 84.0903358 0.0004193343 9.813910e-01 0.995572719 2 110658688 110658742 55 - 1.934 1.940 0.020
ENSG00000169679 E024 77.7662500 0.0006186664 1.321575e-01 0.513627402 2 110659978 110660036 59 - 1.869 1.934 0.218
ENSG00000169679 E025 14.3934254 0.0274357270 4.667563e-06 0.001782159 2 110660868 110661355 488 - 0.826 1.385 2.029
ENSG00000169679 E026 8.1787492 0.1197284510 5.311811e-02 0.353008436 2 110661356 110661581 226 - 0.736 1.100 1.383
ENSG00000169679 E027 134.7454269 0.0041599768 7.604345e-01 0.935978913 2 110661582 110661841 260 - 2.129 2.148 0.062
ENSG00000169679 E028 1.9812594 0.0716313111 1.161411e-01 0.486484308 2 110661842 110661989 148 - 0.283 0.599 1.694
ENSG00000169679 E029 1.7635831 0.0115763851 6.623163e-01 0.901358061 2 110665962 110666262 301 - 0.399 0.477 0.405
ENSG00000169679 E030 109.1350865 0.0003315597 4.922340e-01 0.825600561 2 110666263 110666414 152 - 2.032 2.061 0.096
ENSG00000169679 E031 114.7914701 0.0002950275 7.461293e-01 0.931558672 2 110667521 110667705 185 - 2.072 2.067 -0.015
ENSG00000169679 E032 59.5354372 0.0005081554 1.370674e-01 0.520909238 2 110667797 110667849 53 - 1.818 1.758 -0.205
ENSG00000169679 E033 84.8557608 0.0003347100 2.280750e-01 0.633257578 2 110669453 110669553 101 - 1.960 1.920 -0.133
ENSG00000169679 E034 63.1041868 0.0023550472 1.115867e-01 0.478456933 2 110670525 110670561 37 - 1.854 1.779 -0.252
ENSG00000169679 E035 45.6417529 0.0019241998 1.443625e-01 0.531308519 2 110670562 110670568 7 - 1.717 1.642 -0.252
ENSG00000169679 E036 96.3498821 0.0070059240 5.138883e-01 0.837093442 2 110672661 110672857 197 - 2.006 1.986 -0.067
ENSG00000169679 E037 37.9584320 0.0267830139 7.219138e-01 0.922760633 2 110674086 110674115 30 - 1.597 1.598 0.004
ENSG00000169679 E038 60.0412513 0.0072266278 7.912888e-01 0.945465513 2 110674116 110674224 109 - 1.791 1.793 0.005
ENSG00000169679 E039 45.6636024 0.0006983036 4.810986e-01 0.819848956 2 110674306 110674365 60 - 1.692 1.662 -0.102
ENSG00000169679 E040 0.3435364 0.5981574046 3.150506e-01   2 110674927 110675297 371 - 0.000 0.223 10.521
ENSG00000169679 E041 0.0000000       2 110676533 110676576 44 -      
ENSG00000169679 E042 0.0000000       2 110676577 110676623 47 -      
ENSG00000169679 E043 0.1795728 0.0619217292 4.510324e-01   2 110677689 110677969 281 - 0.000 0.125 9.512
ENSG00000169679 E044 40.4514943 0.0007835680 8.390219e-01 0.959893514 2 110677970 110678063 94 - 1.626 1.622 -0.014