ENSG00000166483

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000450114 ENSG00000166483 HEK293_DMSO_6hA HEK293_OSMI2_6hA WEE1 protein_coding protein_coding 25.10768 18.36951 16.37905 3.311633 1.857704 -0.1653661 9.762746 4.633183 6.710452 0.9091150 0.9905696 0.5334442 0.3821542 0.2522000 0.4061000 0.15390000 5.589391e-03 1.918926e-10 FALSE TRUE
ENST00000530175 ENSG00000166483 HEK293_DMSO_6hA HEK293_OSMI2_6hA WEE1 protein_coding nonsense_mediated_decay 25.10768 18.36951 16.37905 3.311633 1.857704 -0.1653661 1.384760 1.803097 0.000000 0.2414936 0.0000000 -7.5023120 0.0662000 0.1076667 0.0000000 -0.10766667 1.918926e-10 1.918926e-10 TRUE TRUE
ENST00000530712 ENSG00000166483 HEK293_DMSO_6hA HEK293_OSMI2_6hA WEE1 protein_coding protein_coding 25.10768 18.36951 16.37905 3.311633 1.857704 -0.1653661 2.761863 4.044045 2.599672 0.9319018 0.6528121 -0.6354939 0.1165042 0.2165000 0.1622667 -0.05423333 7.030808e-01 1.918926e-10 FALSE TRUE
ENST00000532275 ENSG00000166483 HEK293_DMSO_6hA HEK293_OSMI2_6hA WEE1 protein_coding retained_intron 25.10768 18.36951 16.37905 3.311633 1.857704 -0.1653661 4.255510 2.806128 1.724337 0.6463434 0.9012684 -0.6993288 0.1561292 0.1518333 0.1137667 -0.03806667 7.586692e-01 1.918926e-10 FALSE FALSE
ENST00000681684 ENSG00000166483 HEK293_DMSO_6hA HEK293_OSMI2_6hA WEE1 protein_coding protein_coding 25.10768 18.36951 16.37905 3.311633 1.857704 -0.1653661 4.065468 3.368550 4.660844 0.8582887 1.2932653 0.4672790 0.1799667 0.1837000 0.2736667 0.08996667 5.996752e-01 1.918926e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000166483 E001 75.6014089 0.0004556228 0.0013804106 0.06509902 11 9573670 9574509 840 + 1.936 1.834 -0.342
ENSG00000166483 E002 1.9443177 0.0466603014 0.7272540789 0.92481426 11 9574687 9575375 689 + 0.417 0.504 0.443
ENSG00000166483 E003 0.2669773 0.0267761665 0.9540549681   11 9575490 9575495 6 + 0.120 0.119 -0.020
ENSG00000166483 E004 0.6233207 0.0196281025 0.9371487114   11 9575496 9575887 392 + 0.214 0.212 -0.022
ENSG00000166483 E005 121.1453350 0.0002826235 0.2861180770 0.68847240 11 9575888 9576093 206 + 2.088 2.081 -0.024
ENSG00000166483 E006 103.1493617 0.0002994052 0.0510446596 0.34697512 11 9576230 9576293 64 + 2.036 1.996 -0.134
ENSG00000166483 E007 146.2716854 0.0002313295 0.0009403356 0.05209582 11 9576487 9576659 173 + 2.200 2.132 -0.227
ENSG00000166483 E008 99.5411577 0.0004309669 0.1447484429 0.53183122 11 9577142 9577263 122 + 2.014 1.989 -0.083
ENSG00000166483 E009 14.5244324 0.0126718994 0.0086297746 0.16216474 11 9577264 9577784 521 + 1.011 1.310 1.068
ENSG00000166483 E010 4.3774322 0.1089209529 0.4061811132 0.77760935 11 9577785 9577796 12 + 0.620 0.819 0.819
ENSG00000166483 E011 7.1629886 0.1393061232 0.4174885393 0.78495382 11 9577797 9577895 99 + 0.781 1.009 0.869
ENSG00000166483 E012 7.9806012 0.1990503758 0.3015061939 0.70108577 11 9577896 9578027 132 + 0.760 1.078 1.206
ENSG00000166483 E013 90.4934817 0.1285289354 0.0250239141 0.25877982 11 9578028 9581226 3199 + 1.686 2.110 1.427
ENSG00000166483 E014 22.6952868 0.0588588056 0.0127053708 0.19437952 11 9581227 9581531 305 + 1.139 1.507 1.284
ENSG00000166483 E015 69.2359943 0.0004418856 0.9254176823 0.98227119 11 9581532 9581543 12 + 1.833 1.856 0.076
ENSG00000166483 E016 146.2354755 0.0002209341 0.0811505042 0.41998091 11 9581544 9581678 135 + 2.177 2.154 -0.077
ENSG00000166483 E017 3.9088967 0.0637344242 0.0600197317 0.37165268 11 9585183 9585257 75 + 0.466 0.822 1.549
ENSG00000166483 E018 136.9145096 0.0002192158 0.6230282923 0.88534868 11 9585258 9585353 96 + 2.115 2.157 0.138
ENSG00000166483 E019 143.5983106 0.0002286582 0.2148803315 0.61946739 11 9585442 9585527 86 + 2.164 2.156 -0.030
ENSG00000166483 E020 108.6765458 0.0003430001 0.0940415245 0.44614681 11 9586449 9586497 49 + 2.055 2.025 -0.099
ENSG00000166483 E021 153.6941161 0.0002049758 0.5516853198 0.85477670 11 9586498 9586619 122 + 2.182 2.192 0.034
ENSG00000166483 E022 74.1395089 0.0004417514 0.8561648564 0.96450008 11 9586711 9586713 3 + 1.865 1.883 0.063
ENSG00000166483 E023 165.5721674 0.0002143930 0.3780796363 0.75869746 11 9586714 9586856 143 + 2.219 2.222 0.010
ENSG00000166483 E024 145.1408618 0.0018150712 0.3610396865 0.74745175 11 9588449 9588537 89 + 2.169 2.164 -0.018
ENSG00000166483 E025 632.3653976 0.0043476534 0.3516338190 0.74076970 11 9588538 9589985 1448 + 2.776 2.820 0.146
ENSG00000166483 E026 1.9101815 0.1295972043 0.3766735484 0.75773873 11 9593225 9593457 233 + 0.352 0.549 1.024