ENSG00000162599

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000357977 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding protein_coding 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.15142025 0.48899365 0.4260700 0.24783962 0.4260700 -0.1944617 0.072429167 0.25670000 0.15276667 -0.10393333 7.270358e-01 1.161582e-05 FALSE TRUE
ENST00000371185 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding protein_coding 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.02572742 0.00000000 0.1303474 0.00000000 0.1303474 3.8109306 0.009358333 0.00000000 0.05160000 0.05160000 7.464799e-01 1.161582e-05 FALSE TRUE
ENST00000371187 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding protein_coding 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.55463220 0.58964560 1.0763075 0.18962912 0.3732012 0.8572506 0.214612500 0.25253333 0.44156667 0.18903333 5.378407e-01 1.161582e-05 FALSE TRUE
ENST00000403491 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding protein_coding 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.60931024 0.55939239 0.0000000 0.18626353 0.0000000 -5.8313513 0.237500000 0.23926667 0.00000000 -0.23926667 1.161582e-05 1.161582e-05 FALSE TRUE
ENST00000476646 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding protein_coding 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.44000767 0.00000000 0.0000000 0.00000000 0.0000000 0.0000000 0.128291667 0.00000000 0.00000000 0.00000000   1.161582e-05 FALSE FALSE
ENST00000479364 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding processed_transcript 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.18154648 0.04057201 0.0000000 0.04057201 0.0000000 -2.3383393 0.057833333 0.01420000 0.00000000 -0.01420000 7.479615e-01 1.161582e-05 FALSE FALSE
ENST00000493627 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding protein_coding 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.20253745 0.21601966 0.0000000 0.21601966 0.0000000 -4.4983763 0.107370833 0.07563333 0.00000000 -0.07563333 7.585881e-01 1.161582e-05   FALSE
ENST00000496712 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding protein_coding 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.02744352 0.10606012 0.0000000 0.05303688 0.0000000 -3.5368005 0.014183333 0.05553333 0.00000000 -0.05553333 3.627057e-01 1.161582e-05   FALSE
ENST00000664149 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding protein_coding 2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.07939617 0.00000000 0.2103417 0.00000000 0.1362098 4.4616706 0.032433333 0.00000000 0.09986667 0.09986667 2.718261e-01 1.161582e-05 FALSE TRUE
MSTRG.1305.7 ENSG00000162599 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFIA protein_coding   2.593917 2.227609 2.250348 0.3165557 0.4140517 0.01458727 0.04495753 0.00000000 0.2624639 0.00000000 0.2624639 4.7679934 0.026000000 0.00000000 0.18273333 0.18273333 7.695790e-01 1.161582e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000162599 E001 0.0000000       1 60865259 60865331 73 +      
ENSG00000162599 E002 0.0000000       1 60865332 60865466 135 +      
ENSG00000162599 E003 0.0000000       1 60973746 60973951 206 +      
ENSG00000162599 E004 0.0000000       1 61053830 61053886 57 +      
ENSG00000162599 E005 0.6652648 0.019577951 0.295780679   1 61077274 61077628 355 + 0.114 0.302 1.748
ENSG00000162599 E006 0.1544607 0.032099164 0.470570647   1 61081767 61081861 95 + 0.000 0.126 15.655
ENSG00000162599 E007 0.1544607 0.032099164 0.470570647   1 61081862 61081915 54 + 0.000 0.126 15.655
ENSG00000162599 E008 0.1544607 0.032099164 0.470570647   1 61081916 61082047 132 + 0.000 0.126 15.655
ENSG00000162599 E009 0.0000000       1 61082143 61082180 38 +      
ENSG00000162599 E010 0.1125166 0.032515910 0.509821314   1 61082398 61082422 25 + 0.113 0.000 -16.008
ENSG00000162599 E011 1.5661941 0.014719229 0.005600435   1 61082423 61082560 138 + 0.600 0.126 -3.150
ENSG00000162599 E012 2.3138609 0.011543401 0.170319317 0.56736819 1 61082561 61082620 60 + 0.631 0.428 -0.966
ENSG00000162599 E013 5.8052505 0.012846293 0.671720093 0.90482932 1 61082621 61082722 102 + 0.860 0.827 -0.131
ENSG00000162599 E014 4.3755201 0.005780150 0.893055898 0.97442449 1 61082723 61082747 25 + 0.715 0.756 0.167
ENSG00000162599 E015 3.9787188 0.005689226 0.751744138 0.93309341 1 61082748 61082753 6 + 0.662 0.729 0.281
ENSG00000162599 E016 4.0940376 0.005494871 0.607389551 0.87862338 1 61082754 61082760 7 + 0.662 0.756 0.390
ENSG00000162599 E017 7.2564399 0.033465737 0.456631630 0.80698169 1 61082761 61082818 58 + 0.871 0.987 0.437
ENSG00000162599 E018 18.1687095 0.012664660 0.775653507 0.94110878 1 61088149 61088411 263 + 1.257 1.310 0.189
ENSG00000162599 E019 19.4402239 0.001572716 0.861480981 0.96621074 1 61088412 61088680 269 + 1.304 1.318 0.049
ENSG00000162599 E020 5.7003597 0.134899509 0.288918580 0.69053841 1 61088681 61111370 22690 + 0.672 0.935 1.041
ENSG00000162599 E021 0.1795728 0.036015126 0.468655275   1 61111371 61111450 80 + 0.000 0.126 15.638
ENSG00000162599 E022 0.4884942 0.022106545 0.078866676   1 61111451 61112256 806 + 0.000 0.302 17.193
ENSG00000162599 E023 0.0000000       1 61112257 61112343 87 +      
ENSG00000162599 E024 9.3486540 0.062840657 0.010279803 0.17567119 1 61112344 61132694 20351 + 0.749 1.169 1.577
ENSG00000162599 E025 0.8822549 0.219381187 0.032210704   1 61132695 61133028 334 + 0.000 0.432 17.361
ENSG00000162599 E026 0.0000000       1 61203523 61203558 36 +      
ENSG00000162599 E027 0.3485388 0.033432944 0.972952165   1 61234728 61234784 57 + 0.114 0.126 0.163
ENSG00000162599 E028 0.0000000       1 61275142 61275332 191 +      
ENSG00000162599 E029 0.0000000       1 61277151 61277293 143 +      
ENSG00000162599 E030 0.2937266 0.027442404 0.215631501   1 61277306 61277519 214 + 0.204 0.000 -16.983
ENSG00000162599 E031 10.4045473 0.099733323 0.827619184 0.95631593 1 61277520 61277585 66 + 1.016 1.098 0.299
ENSG00000162599 E032 11.5890109 0.051954779 0.260764667 0.66610198 1 61332512 61332586 75 + 1.160 1.040 -0.431
ENSG00000162599 E033 12.1728653 0.055997772 0.194878515 0.59767472 1 61352450 61352567 118 + 1.190 1.052 -0.497
ENSG00000162599 E034 11.7262305 0.002557385 0.007323918 0.15051955 1 61359147 61359274 128 + 1.218 0.988 -0.831
ENSG00000162599 E035 0.0000000       1 61372697 61372744 48 +      
ENSG00000162599 E036 13.2850624 0.003150320 0.000543983 0.03793892 1 61383237 61383365 129 + 1.284 0.988 -1.064
ENSG00000162599 E037 0.9684943 0.015259117 0.952920540   1 61385871 61385924 54 + 0.278 0.303 0.165
ENSG00000162599 E038 1.0229243 0.043508326 0.641015229   1 61399376 61399520 145 + 0.276 0.370 0.599
ENSG00000162599 E039 0.0000000       1 61404076 61404103 28 +      
ENSG00000162599 E040 14.8268850 0.039935285 0.656695279 0.89937120 1 61404104 61404282 179 + 1.224 1.194 -0.106
ENSG00000162599 E041 11.1545449 0.002371696 0.424472615 0.78900210 1 61406562 61406671 110 + 1.036 1.139 0.371
ENSG00000162599 E042 8.5357807 0.004688347 0.851239225 0.96324542 1 61406672 61406727 56 + 0.958 1.003 0.168
ENSG00000162599 E043 7.3970216 0.124369016 0.966566193 0.99240854 1 61426465 61426556 92 + 0.898 0.928 0.115
ENSG00000162599 E044 0.1125166 0.032515910 0.509821314   1 61439451 61439518 68 + 0.113 0.000 -16.008
ENSG00000162599 E045 0.0000000       1 61443523 61443577 55 +      
ENSG00000162599 E046 456.8240179 0.019694733 0.657052235 0.89950540 1 61455303 61462788 7486 + 2.643 2.678 0.119