ENSG00000159917

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000291182 ENSG00000159917 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF235 protein_coding protein_coding 2.163092 1.464978 0.982036 0.3844 0.2951719 -0.5722288 0.41704882 0.04790905 0.238661509 0.04790905 0.131096722 2.1023225 0.180725000 0.02146667 0.235566667 0.21410000 0.47304781 0.04368914 FALSE TRUE
ENST00000587921 ENSG00000159917 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF235 protein_coding retained_intron 2.163092 1.464978 0.982036 0.3844 0.2951719 -0.5722288 0.10693492 0.28570707 0.004836924 0.10505594 0.004836924 -4.3169048 0.051100000 0.18520000 0.005733333 -0.17946667 0.04368914 0.04368914 FALSE TRUE
ENST00000589248 ENSG00000159917 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF235 protein_coding protein_coding 2.163092 1.464978 0.982036 0.3844 0.2951719 -0.5722288 0.01004796 0.08038365 0.000000000 0.08038365 0.000000000 -3.1760618 0.008816667 0.07053333 0.000000000 -0.07053333 0.83022228 0.04368914 FALSE FALSE
ENST00000592844 ENSG00000159917 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF235 protein_coding nonsense_mediated_decay 2.163092 1.464978 0.982036 0.3844 0.2951719 -0.5722288 0.25321553 0.15546728 0.117300980 0.07490708 0.072913807 -0.3783025 0.125500000 0.13496667 0.159866667 0.02490000 0.99288852 0.04368914   FALSE
ENST00000650576 ENSG00000159917 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF235 protein_coding protein_coding 2.163092 1.464978 0.982036 0.3844 0.2951719 -0.5722288 0.54810334 0.40889471 0.161451678 0.08531421 0.103452695 -1.2887857 0.274150000 0.28610000 0.240866667 -0.04523333 0.74833992 0.04368914 FALSE TRUE
MSTRG.17280.2 ENSG00000159917 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF235 protein_coding   2.163092 1.464978 0.982036 0.3844 0.2951719 -0.5722288 0.23074331 0.27010224 0.101612329 0.01200293 0.030742333 -1.3274571 0.138566667 0.20463333 0.103866667 -0.10076667 0.67890792 0.04368914 TRUE TRUE
MSTRG.17280.4 ENSG00000159917 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF235 protein_coding   2.163092 1.464978 0.982036 0.3844 0.2951719 -0.5722288 0.07996211 0.21651370 0.000000000 0.21651370 0.000000000 -4.5015264 0.036283333 0.09706667 0.000000000 -0.09706667 0.78277535 0.04368914 FALSE FALSE
MSTRG.17280.5 ENSG00000159917 HEK293_DMSO_6hA HEK293_OSMI2_6hA ZNF235 protein_coding   2.163092 1.464978 0.982036 0.3844 0.2951719 -0.5722288 0.39297207 0.00000000 0.338522101 0.00000000 0.338522101 5.1231782 0.131854167 0.00000000 0.218633333 0.21863333 0.73171857 0.04368914 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000159917 E001 3.4478705 0.049277142 3.724583e-01 0.755025028 19 44228729 44228834 106 - 0.713 0.580 -0.568
ENSG00000159917 E002 2.5296615 0.008595816 9.361370e-01 0.985054111 19 44228835 44228963 129 - 0.520 0.530 0.050
ENSG00000159917 E003 2.0741451 0.152551452 8.152587e-01 0.953047626 19 44234755 44234869 115 - 0.448 0.474 0.133
ENSG00000159917 E004 8.7548929 0.021541999 2.922491e-01 0.693179752 19 44234870 44236109 1240 - 0.872 0.987 0.431
ENSG00000159917 E005 2.8261003 0.014141720 5.082247e-02 0.346288700 19 44236110 44236349 240 - 0.284 0.630 1.819
ENSG00000159917 E006 4.0292835 0.007898692 7.043340e-02 0.396596847 19 44236350 44236729 380 - 0.455 0.745 1.302
ENSG00000159917 E007 2.5214423 0.009078561 2.344505e-01 0.639457379 19 44274526 44274587 62 - 0.378 0.582 1.027
ENSG00000159917 E008 0.9836890 0.015903708 4.045692e-01   19 44274752 44274802 51 - 0.165 0.319 1.230
ENSG00000159917 E009 1.1922114 0.017512944 1.786638e-01   19 44274803 44274921 119 - 0.456 0.218 -1.512
ENSG00000159917 E010 0.1717682 0.040232136 1.000000e+00   19 44286348 44286363 16 - 0.000 0.085 9.095
ENSG00000159917 E011 42.9345114 0.035322252 5.082532e-01 0.833875879 19 44286364 44288804 2441 - 1.629 1.596 -0.114
ENSG00000159917 E012 8.9417664 0.031698835 7.869180e-01 0.944234443 19 44288805 44289196 392 - 0.989 0.963 -0.099
ENSG00000159917 E013 6.8511895 0.003909411 9.509258e-06 0.002950879 19 44289197 44290566 1370 - 0.284 0.986 3.232
ENSG00000159917 E014 1.0670685 0.014833493 1.808643e-01   19 44298808 44298819 12 - 0.454 0.218 -1.503
ENSG00000159917 E015 4.7904058 0.005667628 2.590944e-01 0.664580207 19 44298820 44298903 84 - 0.845 0.691 -0.619
ENSG00000159917 E016 8.7775749 0.005948444 1.178077e-01 0.489599479 19 44299606 44299732 127 - 1.067 0.888 -0.663
ENSG00000159917 E017 7.3035737 0.050418089 3.059280e-01 0.704608930 19 44303390 44303452 63 - 1.000 0.808 -0.730
ENSG00000159917 E018 0.1812101 0.135570087 2.342005e-01   19 44304701 44304970 270 - 0.166 0.000 -11.266
ENSG00000159917 E019 4.5005119 0.009190556 3.655138e-01 0.750559516 19 44304971 44305046 76 - 0.786 0.651 -0.554