ENSG00000159445

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368814 ENSG00000159445 HEK293_DMSO_6hA HEK293_OSMI2_6hA THEM4 protein_coding protein_coding 21.30184 14.65688 13.46666 2.160446 0.8387399 -0.1220992 3.277307 2.3687461 1.8821039 0.4520368 0.1099463 -0.33020997 0.1561542 0.16056667 0.1409000 -0.019666667 0.76472678 0.02330811 FALSE TRUE
ENST00000471464 ENSG00000159445 HEK293_DMSO_6hA HEK293_OSMI2_6hA THEM4 protein_coding nonsense_mediated_decay 21.30184 14.65688 13.46666 2.160446 0.8387399 -0.1220992 5.214731 2.0359359 2.9551358 0.2987746 0.7025751 0.53533725 0.2434375 0.14436667 0.2266333 0.082266667 0.71295156 0.02330811 FALSE TRUE
ENST00000477437 ENSG00000159445 HEK293_DMSO_6hA HEK293_OSMI2_6hA THEM4 protein_coding processed_transcript 21.30184 14.65688 13.46666 2.160446 0.8387399 -0.1220992 2.377800 4.7762760 2.5463714 0.9122628 0.5950523 -0.90480614 0.1282292 0.32113333 0.1853000 -0.135833333 0.18005249 0.02330811 FALSE FALSE
ENST00000483207 ENSG00000159445 HEK293_DMSO_6hA HEK293_OSMI2_6hA THEM4 protein_coding retained_intron 21.30184 14.65688 13.46666 2.160446 0.8387399 -0.1220992 1.539298 3.0984076 1.7421814 0.5719342 0.5226601 -0.82702360 0.0803000 0.20890000 0.1257000 -0.083200000 0.33435761 0.02330811 FALSE TRUE
ENST00000489410 ENSG00000159445 HEK293_DMSO_6hA HEK293_OSMI2_6hA THEM4 protein_coding protein_coding 21.30184 14.65688 13.46666 2.160446 0.8387399 -0.1220992 1.878605 1.3187360 1.4990810 0.2831174 0.5253506 0.18361582 0.0911125 0.08986667 0.1077000 0.017833333 0.92360271 0.02330811 FALSE FALSE
MSTRG.2239.3 ENSG00000159445 HEK293_DMSO_6hA HEK293_OSMI2_6hA THEM4 protein_coding   21.30184 14.65688 13.46666 2.160446 0.8387399 -0.1220992 3.526858 0.5379099 2.4109236 0.1576547 0.5906079 2.14354689 0.1585458 0.03503333 0.1852333 0.150200000 0.02330811 0.02330811 FALSE TRUE
MSTRG.2239.7 ENSG00000159445 HEK293_DMSO_6hA HEK293_OSMI2_6hA THEM4 protein_coding   21.30184 14.65688 13.46666 2.160446 0.8387399 -0.1220992 3.314485 0.4017274 0.3871543 0.2626715 0.3871543 -0.05198953 0.1344667 0.03326667 0.0256000 -0.007666667 0.75317231 0.02330811 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000159445 E001 182.3529762 0.0033289063 0.0166799442 0.21867222 1 151870866 151874054 3189 - 2.193 2.309 0.389
ENSG00000159445 E002 102.8625053 0.0204443976 0.9491463636 0.98810996 1 151874055 151874359 305 - 1.995 2.032 0.122
ENSG00000159445 E003 50.6291425 0.0142027963 0.7916306170 0.94554879 1 151874360 151874376 17 - 1.704 1.715 0.038
ENSG00000159445 E004 90.4618292 0.0004202458 0.7344280405 0.92728230 1 151874377 151874531 155 - 1.944 1.968 0.081
ENSG00000159445 E005 161.7291603 0.0006205374 0.0159459503 0.21457707 1 151874532 151874928 397 - 2.226 2.196 -0.101
ENSG00000159445 E006 64.1448740 0.0097223367 0.1413976600 0.52721159 1 151877001 151877086 86 - 1.842 1.785 -0.191
ENSG00000159445 E007 49.1858793 0.0005980716 0.1375009161 0.52147280 1 151877087 151877125 39 - 1.715 1.679 -0.123
ENSG00000159445 E008 89.8066345 0.0011585230 0.1819909780 0.58199370 1 151888273 151888383 111 - 1.964 1.949 -0.051
ENSG00000159445 E009 107.1555469 0.0003656608 0.0737093324 0.40359547 1 151889214 151889373 160 - 2.044 2.022 -0.075
ENSG00000159445 E010 49.3899618 0.0562058555 0.0271574449 0.26764797 1 151889374 151889750 377 - 1.535 1.801 0.905
ENSG00000159445 E011 37.6199850 0.0076987013 0.2715128797 0.67552902 1 151889983 151890214 232 - 1.527 1.636 0.373
ENSG00000159445 E012 104.4703339 0.0736568196 0.0361093810 0.30100514 1 151890215 151891440 1226 - 1.872 2.116 0.818
ENSG00000159445 E013 17.6739279 0.0503131741 0.4721601334 0.81503909 1 151894830 151895007 178 - 1.309 1.217 -0.324
ENSG00000159445 E014 69.0273109 0.0005371394 0.0003277962 0.02808623 1 151895008 151895194 187 - 1.899 1.786 -0.380
ENSG00000159445 E015 0.3485388 0.0279648312 0.9233892214   1 151896914 151896963 50 - 0.122 0.117 -0.068
ENSG00000159445 E016 7.9424123 0.0043999705 0.0871603756 0.43235523 1 151907321 151907406 86 - 1.018 0.857 -0.607
ENSG00000159445 E017 0.7946133 0.5226938749 0.5473881443   1 151907407 151907648 242 - 0.125 0.342 1.840
ENSG00000159445 E018 23.7732251 0.0014858187 0.0007153716 0.04493689 1 151909360 151909637 278 - 1.493 1.292 -0.697