ENSG00000159335

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000309083 ENSG00000159335 HEK293_DMSO_6hA HEK293_OSMI2_6hA PTMS protein_coding protein_coding 198.3768 138.0686 286.7098 20.24106 67.80589 1.054152 131.52800 91.20960 214.77598 15.265940 53.21484 1.2354842 0.7034000 0.6557667 0.7445667 0.0888 0.02158594 0.02158594 FALSE FALSE
ENST00000540667 ENSG00000159335 HEK293_DMSO_6hA HEK293_OSMI2_6hA PTMS protein_coding processed_transcript 198.3768 138.0686 286.7098 20.24106 67.80589 1.054152 58.13745 39.37275 59.92145 4.484393 13.27312 0.6057493 0.2496417 0.2898667 0.2106667 -0.0792 0.04119399 0.02158594 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000159335 E001 0.5253094 5.296691e-01 1.0000000000   12 6765516 6765638 123 + 0.173 0.156 -0.180
ENSG00000159335 E002 28.5206187 1.272398e-03 0.0207530057 0.23931523 12 6766363 6766372 10 + 1.525 1.362 -0.561
ENSG00000159335 E003 30.0196049 1.848820e-03 0.0013979540 0.06553330 12 6766373 6766374 2 + 1.564 1.337 -0.783
ENSG00000159335 E004 58.6797891 1.119482e-02 0.0005627259 0.03872816 12 6766375 6766378 4 + 1.850 1.598 -0.854
ENSG00000159335 E005 78.8507222 9.290825e-03 0.0003813748 0.03047119 12 6766379 6766397 19 + 1.970 1.738 -0.780
ENSG00000159335 E006 96.6357237 6.494837e-03 0.0001884725 0.01970447 12 6766398 6766430 33 + 2.049 1.845 -0.684
ENSG00000159335 E007 1183.9875041 5.915050e-04 0.0034171944 0.10517730 12 6766431 6766750 320 + 3.076 3.039 -0.123
ENSG00000159335 E008 13.2618093 1.839314e-01 0.0032658826 0.10289977 12 6769053 6769177 125 + 0.793 1.420 2.281
ENSG00000159335 E009 1610.4117417 6.230102e-05 0.2250339950 0.63012821 12 6769603 6769674 72 + 3.202 3.191 -0.035
ENSG00000159335 E010 67.2550156 1.060782e-02 0.3463033782 0.73630736 12 6769844 6769920 77 + 1.787 1.864 0.259
ENSG00000159335 E011 1084.9114373 1.401234e-03 0.9601154977 0.99081651 12 6769921 6769923 3 + 3.016 3.032 0.054
ENSG00000159335 E012 2304.2459329 1.556974e-04 0.9722247827 0.99342022 12 6769924 6769999 76 + 3.350 3.355 0.016
ENSG00000159335 E013 1459.1524518 9.290008e-04 0.1219571846 0.49712011 12 6770157 6770168 12 + 3.137 3.173 0.120
ENSG00000159335 E014 1295.7066873 1.142589e-03 0.0530496609 0.35280582 12 6770169 6770171 3 + 3.082 3.127 0.149
ENSG00000159335 E015 2003.2509241 4.927992e-05 0.4089767177 0.77967745 12 6770172 6770218 47 + 3.294 3.288 -0.021
ENSG00000159335 E016 3840.6678776 7.293138e-04 0.2429027596 0.64792540 12 6770392 6770952 561 + 3.562 3.588 0.086