ENSG00000154945

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000285243 ENSG00000154945 HEK293_DMSO_6hA HEK293_OSMI2_6hA ANKRD40 protein_coding protein_coding 24.28458 13.05074 16.97176 2.039466 3.135849 0.3787493 23.5797583 11.898613 16.4389494 1.91471736 3.16981977 0.465990 0.96680417 0.91046667 0.96483333 0.05436667 0.005727376 0.005727376 FALSE  
ENST00000513072 ENSG00000154945 HEK293_DMSO_6hA HEK293_OSMI2_6hA ANKRD40 protein_coding protein_coding 24.28458 13.05074 16.97176 2.039466 3.135849 0.3787493 0.5912427 1.087277 0.4178111 0.09887826 0.02398874 -1.358882 0.02877917 0.08513333 0.02736667 -0.05776667 0.049848610 0.005727376 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000154945 E001 1249.5934608 0.0034149861 6.916683e-01 0.912332347 17 50693198 50696143 2946 - 3.094 3.100 0.018
ENSG00000154945 E002 157.6328668 0.0007550642 3.785188e-01 0.759065406 17 50696940 50697121 182 - 2.209 2.187 -0.073
ENSG00000154945 E003 162.1792473 0.0002530215 2.336198e-01 0.638555772 17 50699399 50699576 178 - 2.226 2.197 -0.098
ENSG00000154945 E004 197.0592081 0.0029768385 6.504324e-01 0.896649537 17 50699577 50699893 317 - 2.304 2.291 -0.045
ENSG00000154945 E005 0.9480634 0.3258484891 8.790903e-01   17 50700285 50700567 283 - 0.313 0.312 -0.006
ENSG00000154945 E006 135.9863177 0.0006758250 1.986267e-01 0.601773552 17 50700568 50700716 149 - 2.116 2.159 0.145
ENSG00000154945 E007 12.4764366 0.0023632014 8.202424e-06 0.002659913 17 50700717 50701098 382 - 0.863 1.300 1.591
ENSG00000154945 E008 90.6059960 0.0004220830 3.885732e-01 0.766135360 17 50707521 50707914 394 - 1.972 1.943 -0.096