ENSG00000154548

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000452027 ENSG00000154548 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF12 protein_coding protein_coding 3.430789 1.594532 1.819534 0.3265432 0.2101163 0.1893239 2.2676434 0.8675693 1.3019391 0.24765234 0.2296320 0.5801159 0.65283333 0.53166667 0.7051667 0.17350000 0.463280230 0.004430878 FALSE TRUE
ENST00000475068 ENSG00000154548 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF12 protein_coding nonsense_mediated_decay 3.430789 1.594532 1.819534 0.3265432 0.2101163 0.1893239 0.6828168 0.3344048 0.3055336 0.05001993 0.1606702 -0.1263113 0.18856667 0.21510000 0.1500333 -0.06506667 0.753172310 0.004430878   FALSE
ENST00000517387 ENSG00000154548 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF12 protein_coding nonsense_mediated_decay 3.430789 1.594532 1.819534 0.3265432 0.2101163 0.1893239 0.2542107 0.1104809 0.1619734 0.05561665 0.1619734 0.5133812 0.08319583 0.06376667 0.1149333 0.05116667 0.794376053 0.004430878   FALSE
ENST00000524221 ENSG00000154548 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF12 protein_coding retained_intron 3.430789 1.594532 1.819534 0.3265432 0.2101163 0.1893239 0.1077164 0.2121151 0.0000000 0.03174730 0.0000000 -4.4732356 0.04009167 0.13863333 0.0000000 -0.13863333 0.004430878 0.004430878   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000154548 E001 67.102069 0.001421951 0.0273616113 0.26851784 6 89095959 89098852 2894 - 1.795 1.883 0.295
ENSG00000154548 E002 26.288962 0.001302757 0.9280499271 0.98289165 6 89098853 89098936 84 - 1.445 1.442 -0.011
ENSG00000154548 E003 17.187439 0.001580510 0.7012829055 0.91556898 6 89098937 89098947 11 - 1.277 1.249 -0.097
ENSG00000154548 E004 19.625018 0.001774636 0.9871516976 0.99683679 6 89105119 89105144 26 - 1.317 1.318 0.003
ENSG00000154548 E005 31.118036 0.001196274 0.3583109792 0.74559570 6 89105145 89105260 116 - 1.488 1.543 0.188
ENSG00000154548 E006 1.166064 0.013524254 0.0008890897   6 89105261 89105426 166 - 0.000 0.564 11.814
ENSG00000154548 E007 21.427466 0.010035080 0.8156855507 0.95316510 6 89105427 89105480 54 - 1.343 1.363 0.070
ENSG00000154548 E008 16.198319 0.002324573 0.1964415069 0.59960813 6 89105481 89105530 50 - 1.283 1.179 -0.367
ENSG00000154548 E009 1.066506 0.014017351 0.3968928830   6 89105531 89105538 8 - 0.253 0.402 0.947
ENSG00000154548 E010 1.238274 0.013129858 0.2270135871   6 89105539 89105550 12 - 0.253 0.463 1.269
ENSG00000154548 E011 1.360142 0.013841438 0.0069980690   6 89105551 89105966 416 - 0.102 0.564 3.337
ENSG00000154548 E012 6.084230 0.003762777 0.5241383342 0.84224120 6 89106681 89107024 344 - 0.815 0.895 0.308
ENSG00000154548 E013 1.300981 0.013860045 0.6441873229   6 89107025 89107027 3 - 0.411 0.331 -0.466
ENSG00000154548 E014 17.461643 0.017477570 0.0078977936 0.15537641 6 89107154 89107258 105 - 1.354 1.094 -0.921
ENSG00000154548 E015 16.554791 0.002818944 0.0016957675 0.07304495 6 89117823 89118071 249 - 1.334 1.067 -0.950