ENSG00000153933

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000284061 ENSG00000153933 HEK293_DMSO_6hA HEK293_OSMI2_6hA DGKE protein_coding protein_coding 3.814644 1.546446 1.102639 0.4324613 0.09646998 -0.4842702 2.48747563 0.66048676 0.80328382 0.197747729 0.051107178 0.27855006 0.62000833 0.43080000 0.74706667 0.316266667 0.299066820 0.001040301 FALSE TRUE
ENST00000570738 ENSG00000153933 HEK293_DMSO_6hA HEK293_OSMI2_6hA DGKE protein_coding protein_coding 3.814644 1.546446 1.102639 0.4324613 0.09646998 -0.4842702 0.13248788 0.43805552 0.00000000 0.092361713 0.000000000 -5.48560562 0.10784583 0.30870000 0.00000000 -0.308700000 0.001040301 0.001040301 FALSE FALSE
ENST00000571084 ENSG00000153933 HEK293_DMSO_6hA HEK293_OSMI2_6hA DGKE protein_coding retained_intron 3.814644 1.546446 1.102639 0.4324613 0.09646998 -0.4842702 0.13374897 0.06052505 0.12832818 0.001037955 0.128328178 0.97188737 0.03582500 0.04486667 0.09963333 0.054766667 0.988975097 0.001040301 FALSE FALSE
ENST00000572810 ENSG00000153933 HEK293_DMSO_6hA HEK293_OSMI2_6hA DGKE protein_coding protein_coding 3.814644 1.546446 1.102639 0.4324613 0.09646998 -0.4842702 0.23209822 0.07738843 0.07279278 0.032569196 0.007397681 -0.07793734 0.07584583 0.04626667 0.06760000 0.021333333 0.891524472 0.001040301 FALSE TRUE
ENST00000572944 ENSG00000153933 HEK293_DMSO_6hA HEK293_OSMI2_6hA DGKE protein_coding protein_coding 3.814644 1.546446 1.102639 0.4324613 0.09646998 -0.4842702 0.27460634 0.10773680 0.03247505 0.028488209 0.032475047 -1.47087789 0.05994167 0.07446667 0.03073333 -0.043733333 0.738983504 0.001040301 FALSE TRUE
MSTRG.14717.1 ENSG00000153933 HEK293_DMSO_6hA HEK293_OSMI2_6hA DGKE protein_coding   3.814644 1.546446 1.102639 0.4324613 0.09646998 -0.4842702 0.36724771 0.05944050 0.04570829 0.036242840 0.022854302 -0.31788523 0.06469167 0.03533333 0.03936667 0.004033333 0.911293092 0.001040301 TRUE TRUE
MSTRG.14717.5 ENSG00000153933 HEK293_DMSO_6hA HEK293_OSMI2_6hA DGKE protein_coding   3.814644 1.546446 1.102639 0.4324613 0.09646998 -0.4842702 0.04028571 0.12372873 0.00000000 0.123728732 0.000000000 -3.74123756 0.01034583 0.05160000 0.00000000 -0.051600000 0.757013971 0.001040301 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000153933 E001 0.0000000       17 56834107 56834130 24 +      
ENSG00000153933 E002 0.1544607 0.032649190 0.672853341   17 56834131 56834150 20 + 0.000 0.106 8.344
ENSG00000153933 E003 2.1849601 0.009186155 0.491724295 0.82533796 17 56834151 56834260 110 + 0.549 0.468 -0.391
ENSG00000153933 E004 4.3603518 0.005559083 0.037903039 0.30675823 17 56834778 56834965 188 + 0.855 0.607 -1.019
ENSG00000153933 E005 6.7658182 0.003588151 0.017269760 0.22218593 17 56834966 56835259 294 + 1.018 0.777 -0.919
ENSG00000153933 E006 3.2205106 0.006595337 0.709013628 0.91837452 17 56835260 56836466 1207 + 0.630 0.607 -0.102
ENSG00000153933 E007 8.8549083 0.009687150 0.307243297 0.70557279 17 56844019 56844178 160 + 1.021 0.942 -0.293
ENSG00000153933 E008 9.5240576 0.002709419 0.528452505 0.84422081 17 56845690 56845809 120 + 1.033 1.006 -0.100
ENSG00000153933 E009 2.3364019 0.273586177 0.635401916 0.89041406 17 56845810 56846302 493 + 0.610 0.423 -0.900
ENSG00000153933 E010 0.7749791 0.024041932 0.393641280   17 56847360 56847429 70 + 0.320 0.191 -0.978
ENSG00000153933 E011 0.6574601 0.018827425 0.518094815   17 56847516 56847658 143 + 0.135 0.262 1.193
ENSG00000153933 E012 9.8963508 0.003812392 0.774785928 0.94078528 17 56847922 56848065 144 + 1.002 1.071 0.253
ENSG00000153933 E013 10.5631091 0.061412993 0.625975285 0.88647460 17 56848696 56848853 158 + 1.075 1.065 -0.038
ENSG00000153933 E014 6.7165610 0.065677727 0.401855523 0.77470220 17 56849181 56849232 52 + 0.942 0.862 -0.303
ENSG00000153933 E015 8.1317159 0.004419341 0.094565191 0.44731220 17 56856512 56856625 114 + 1.046 0.899 -0.549
ENSG00000153933 E016 0.9668570 0.015378759 0.699083745   17 56856626 56856665 40 + 0.237 0.324 0.612
ENSG00000153933 E017 7.6602848 0.003349315 0.337001498 0.72964593 17 56858594 56858665 72 + 0.971 0.899 -0.270
ENSG00000153933 E018 5.4428224 0.007176059 0.178728665 0.57762048 17 56861791 56861794 4 + 0.877 0.735 -0.560
ENSG00000153933 E019 10.1006378 0.006636229 0.485974141 0.82249154 17 56861795 56861918 124 + 1.062 1.028 -0.122
ENSG00000153933 E020 6.0951696 0.042604755 0.876928644 0.97040486 17 56862140 56862185 46 + 0.833 0.863 0.119
ENSG00000153933 E021 6.3726272 0.004564819 0.312848258 0.71043668 17 56862186 56862251 66 + 0.917 0.833 -0.320
ENSG00000153933 E022 1.3541555 0.012211667 0.841579394   17 56862252 56862611 360 + 0.389 0.377 -0.064
ENSG00000153933 E023 5.2153520 0.007747454 0.385416119 0.76399492 17 56862612 56862644 33 + 0.833 0.756 -0.306
ENSG00000153933 E024 234.3856037 0.007355990 0.001186328 0.05971237 17 56862645 56869567 6923 + 2.302 2.403 0.336