ENSG00000148459

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376215 ENSG00000148459 HEK293_DMSO_6hA HEK293_OSMI2_6hA PDSS1 protein_coding protein_coding 15.53824 8.460453 7.54046 1.783821 0.8105471 -0.1658746 10.8626664 3.7623406 4.5763510 0.7902377 1.24437794 0.2818868 0.66649167 0.44936667 0.5869667 0.13760000 6.803433e-01 9.695625e-05 FALSE  
ENST00000470978 ENSG00000148459 HEK293_DMSO_6hA HEK293_OSMI2_6hA PDSS1 protein_coding processed_transcript 15.53824 8.460453 7.54046 1.783821 0.8105471 -0.1658746 0.1539185 0.7306699 0.0000000 0.2051849 0.00000000 -6.2107588 0.01613750 0.09590000 0.0000000 -0.09590000 9.695625e-05 9.695625e-05 FALSE  
ENST00000473224 ENSG00000148459 HEK293_DMSO_6hA HEK293_OSMI2_6hA PDSS1 protein_coding processed_transcript 15.53824 8.460453 7.54046 1.783821 0.8105471 -0.1658746 1.0779641 0.8659473 0.6298153 0.3020281 0.05461505 -0.4531885 0.07444583 0.09696667 0.0854000 -0.01156667 9.329383e-01 9.695625e-05 FALSE  
ENST00000491711 ENSG00000148459 HEK293_DMSO_6hA HEK293_OSMI2_6hA PDSS1 protein_coding nonsense_mediated_decay 15.53824 8.460453 7.54046 1.783821 0.8105471 -0.1658746 1.2804527 1.7049808 0.8097586 0.3299586 0.11750450 -1.0649214 0.09520417 0.20300000 0.1126333 -0.09036667 3.031760e-01 9.695625e-05 TRUE  
MSTRG.3682.1 ENSG00000148459 HEK293_DMSO_6hA HEK293_OSMI2_6hA PDSS1 protein_coding   15.53824 8.460453 7.54046 1.783821 0.8105471 -0.1658746 2.1632345 1.3965148 1.5245351 0.5228234 0.44174897 0.1256769 0.14770833 0.15476667 0.2149667 0.06020000 7.879344e-01 9.695625e-05 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000148459 E001 1.554873 0.0112416726 2.031100e-01   10 26697678 26697700 23 + 0.508 0.290 -1.226
ENSG00000148459 E002 12.580023 0.0058670556 8.466823e-02 0.427161349 10 26697701 26697840 140 + 1.211 1.046 -0.591
ENSG00000148459 E003 13.966290 0.0022109681 1.941074e-01 0.596582524 10 26702162 26702194 33 + 1.227 1.115 -0.401
ENSG00000148459 E004 27.871529 0.0014157580 1.325751e-01 0.514110601 10 26704677 26704741 65 + 1.511 1.415 -0.329
ENSG00000148459 E005 22.856936 0.0014012760 3.231172e-02 0.288476556 10 26705286 26705307 22 + 1.456 1.308 -0.515
ENSG00000148459 E006 43.086646 0.0007172154 5.294493e-02 0.352503185 10 26705308 26705394 87 + 1.698 1.601 -0.328
ENSG00000148459 E007 58.270817 0.0102134249 1.188800e-01 0.491496954 10 26709638 26709768 131 + 1.817 1.728 -0.299
ENSG00000148459 E008 6.574332 0.0040961220 2.974470e-01 0.697703571 10 26720135 26720217 83 + 0.806 0.934 0.493
ENSG00000148459 E009 56.958163 0.0017502848 4.180964e-01 0.785244809 10 26720218 26720342 125 + 1.783 1.750 -0.111
ENSG00000148459 E010 32.275298 0.0008713400 4.421267e-01 0.798800407 10 26720343 26720359 17 + 1.547 1.505 -0.145
ENSG00000148459 E011 46.511460 0.0026382768 3.492443e-01 0.738739088 10 26723806 26723917 112 + 1.710 1.664 -0.154
ENSG00000148459 E012 58.864758 0.0052162614 9.601700e-01 0.990833116 10 26724014 26724123 110 + 1.783 1.789 0.020
ENSG00000148459 E013 7.401261 0.0244602939 6.614117e-01 0.900997751 10 26724124 26724127 4 + 0.887 0.949 0.233
ENSG00000148459 E014 1.624317 0.0109648762 2.135715e-01   10 26734651 26734723 73 + 0.291 0.507 1.217
ENSG00000148459 E015 54.524427 0.0021345005 2.101063e-01 0.614340226 10 26735240 26735320 81 + 1.783 1.723 -0.204
ENSG00000148459 E016 69.922257 0.0005247636 9.200690e-01 0.980825109 10 26735466 26735579 114 + 1.854 1.860 0.022
ENSG00000148459 E017 22.432616 0.0285161137 2.544812e-02 0.260326589 10 26740645 26740817 173 + 1.226 1.462 0.825
ENSG00000148459 E018 70.834354 0.0004010504 1.134422e-01 0.481639444 10 26742497 26742577 81 + 1.824 1.890 0.221
ENSG00000148459 E019 122.204997 0.0014784722 5.440641e-06 0.001972786 10 26746333 26746910 578 + 2.006 2.157 0.505